产品订购信息：
英文名称  Anti-Scavenger Receptor BII 

中文名称   清道夫受体B2抗体费用 

别    名  SCRB2_HUMAN; Lysosome membrane protein 2; 85 kDa lysosomal membrane sialoglycoprotein; LGP85; CD36 antigen-like 2; Lysosome membrane protein II; LIMP II; Scavenger receptor class B member 2; CD_antigen=CD36; SCARB2; CD36L2; LIMPII; LGP85; LIMP II.

浓    度   1mg/1ml

规 格  0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit 

产品类型   一抗  

研究领域     心 细胞生物 信号转导 转录调节因子 细胞膜受体

蛋白分子量  predicted molecular weight: 52kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human Scavenger Receptor BII 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

清道夫受体B2抗体费用 产品应用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 High density lipoproteins (HDLs) play a critical role in cholesterol metabolism and their plasma concentrations are inversely correlated with risk for atherosclerosis. SR-BI and SR-BII (previously known as SR-BI.2) are the alternatively spliced products of a single gene. SR-BII and SR-BI are identical except for the encoded c-terminal cytoplasmic domain. Both SR-BI and SR-BII bind HDL and mediates selective uptake of HDL cholesteryl ester, but with SR-BII having an approximately 4-fold lower efficiency than SR-BI. SR-BI and SR-BII are expressed primarily in liver and non-placental steroidgenic tissues. Although the role of these scavenger receptors is not completely clear, SR-BII mRNA results from the alternative splicing of SR-BI precursor transcripts with both isoforms mediating selective transfer of lipid between HDL and cells. Therefore, the relative expression and functional activities of these two isoforms create a potential means of regulating selective lipid transfer between HDL and cells.

Function : Acts as a lysosomal receptor for glucosylceramidase (GBA) targeting.

Subunit : Interacts with GBA (via the coiled-coil domain).

Subcellular Location : Lysosome membrane; Multi-pass membrane protein.

DISEASE : Defects in SCARB2 are the cause of progressive myoclonic epilepsy type 4 with or without renal failure (EPM4) [MIM:254900]. An autosomal recessive progressive myoclonic epilepsy associated with renal failure in some cases. Cognitive function is preserved. Myoclonus is a brief, involuntary twitching of a muscle or a group of muscles. Cognitive function is preserved.

Note=Genetic variants in SCARB2 can act as modifier of the phenotypic expression and severity of Gaucher disease.

Similarity : Belongs to the CD36 family.

改良番茄汁琼脂培养基基础2730g用于乳酸菌饮料中乳酸菌的菌落计数

YT Agar 培养基 250g incubation media YT Agar 培养基 250g

赭绿青霉 支/瓶

肠球菌、溶血性链球菌和粪链球菌检验培养基

BCYEAgarBase

改良缓冲蛋白胨水(MBP)250g用于单增李氏菌前增菌培养(SN标准)

甘氨酸培养基 Glycine Medium 用于弯曲杆菌甘氨酸耐受性试验(GB标准)

YPD肉汤 Yeast Extract Peptone Dextrose Broth 250克 BR

奈瑟菌增菌培养基250g/瓶用于奈瑟菌的增菌和转送，每培养基中添加2ncubationmedia奈瑟菌增菌培养基250g/瓶用于奈瑟菌的增菌和转送，每培养基中添加2110

阪崎肠杆菌显色培养基(DFI琼脂) 1000ml 用于阪崎杆菌的显色培养，阪崎杆菌显蓝色，其它肠杆菌显无色。

UVMMedium

Mueller-Hinton琼脂(MHA) Mueller-Hinton Agar 用于弯曲杆菌的药物敏感性试验

山梨醇麦康克琼脂基础 SMAC 250克 大肠杆菌O157：H7的选择性分离培养

MH肉汤250g/瓶用于稀释法进行药敏试验incubationmediaMH肉汤250g/瓶用于稀释法进行药敏试验

西蒙氏枸橼酸盐培养基 250g 用于肠道菌的柠檬酸盐利用试验(GB标准)

CL-0136L6(大鼠成肌细胞)5×106cells/瓶×2

GPT Others Rat 大鼠 GPT1 / GPT 杆状病毒-昆虫细胞裂解液 (阳性对照)

人气管平滑肌细胞裂解物HTSMCL

HPAC细胞，人*癌细胞 人上皮细胞,16HBE细胞 皮肤肥大细胞Many types of cells包装：5 × 105次方(1ml)

兔眼Tenon's囊成纤维细胞;RYTF

TGFBR2 Others Human 人 TGFβR2 / TGFR2 人细胞裂解液 (阳性对照)

清道夫受体B2抗体费用CL-0135L1210(小鼠细胞)5×106cells/瓶×2

CD14 Others Human 人 CD14 人细胞裂解液 (阳性对照)

小鼠小肠内皮细胞完全培养基 100mL

Pig MSC猪骨髓间质干细胞 Pig MSC of porcine bone marrow mesenchymal stem cells DMEM低糖+10% FBS+glutamin

IL6 Protein Rat 重组大鼠 IL6 / Ierleukin-6 蛋白

RM1(大鼠肌肉成纤维样细胞) 5×106cells/瓶×2 PC-12未分化(大鼠肾上腺嗜铬细胞瘤)

抗体的生物素化标记实验要点：

1. 清道夫受体B2抗体费用 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及苯酚基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

抗体的鉴定：

1）清道夫受体B2抗体费用 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。