产品订购信息：
英文名称  Anti-STIL 

中文名称   中断位点蛋白STIL抗体说明书 

别    名  MCPH7; SCL interrupting locus protein; SCL-interrupting locus protein; SCL/TAL1 interrupting locus; SIL; STIL; STIL_HUMAN; TAL 1 interrupting locus protein; TAL-1-interrupting locus protein.

浓    度   1mg/1ml

规 格  0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Cow, Rabbit 

产品类型   一抗  

研究领域     细胞生物 免疫学 神经生物学 信号转导 干细胞 细胞周期蛋白 细胞分化

蛋白分子量  predicted molecular weight: 143kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human STIL 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

中断位点蛋白STIL抗体说明书 产品应用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:20-100 IF=1:50-200

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 TAL1 disruption at 1p32, a common rearrangement in the T-cell acute lymphoblastic leukemia, usually results in the formation of a SCL interrupting locus (SIL)-TAL1 fusion product. SIL is an immediate early gene whose expression is associated with cell proliferation. The Sil protein exhibits ubiquitous expression in hematopoietic cell lines and tissues. However, Sil protein levels remain tightly regulated during the cell cycle, achieving peak levels in mitosis and diminishing on transition to G1 phase. Overexpression of Sil in primary adenocarcinomas predicts metastatic spread, especially in lung tumors with increased mitotic activity.

Function : Immediate-early gene. Plays an important role in embryonic development as well as in cellular growth and proliferation; its long-term silencing affects cell survival and cell cycle distribution as well as decreases CDK1 activity correlated with reduced phosphorylation of CDK1. Plays a role as a positive regulator of the sonic hedgehog pathway, acting downstream of PTCH1.

Subunit : Interacts with PIN1 via its WW domain. This interaction is dependent on STIL mitotic phosphorylation (By similarity).

Subcellular Location : Cytoplasm, cytosol.

Tissue Specificity : Expressed in all hematopoietic tissues and cell lines. Highly expressed in a variety of tumors characterized by increased mitotic activity with highest expression in lung cancer.

Post-translational modifications : Phosphorylated following the activation of the mitotic checkpoint.

DISEASE : Note=A chromosomal aberration involving STIL may be a cause of some T-cell acute lymphoblastic leukemias (T-ALL). A deletion at 1p32 between STIL and TAL1 genes leads to STIL/TAL1 fusion mRNA with STIL exon 1 slicing to TAL1 exon 3. As both STIL exon 1 and TAL1 exon 3 are 5'-untranslated exons, STIL/TAL1 fusion mRNA predicts a full length TAL1 protein under the control of the STIL promoter, leading to inappropriate TAL1 expression. In childhood T-cell malignancies (T-ALL), a type of defect such as STIL/TAL1 fusion is associated with a good prognosis. In cultured lymphocytes from healthy adults, STIL/TAL1 fusion mRNA may be detected after 7 days of culture.

Defects in STIL are the cause of microcephaly primary type 7 (MCPH7) [MIM:612703]. Microcephaly is defined as a head circumference more than 3 standard deviations below the age-related mean. Brain weight is markedly reduced and the cerebral cortex is disproportionately small. Despite this marked reduction in size, the gyral pattern is relatively well preserved, with no major abnormality in cortical architecture. Primary microcephaly is further defined by the absence of other syndromic features or significant neurological deficits.

Database links : UniProtKB/Swiss-Prot: Q15468.2

改良克氏双糖铁培养基250g生化培养基，用于小肠耶尔森氏菌的生化反应筛选

改良MSRV培养基 MSRV Medium，Modified 用于沙门氏的分离培养(MERCK方法)

小牛浸膏粉 Beef extract powder 250克 BR

含糖牛肉汤培养基250g/瓶用于乳酸菌培养和鉴别incubationmedia含糖牛肉汤培养基250g/瓶用于乳酸菌培养和鉴别

Tergitol-7琼脂 250g 用于大肠菌群的鉴定(NMKL)

改良磷酸盐缓冲液PSB250g用于小肠耶尔森氏菌增菌培养

盐增菌液(SF) Selenite Enrichment Medium 用于增殖标本中的沙门氏菌

脱氢胆酸 Sodium dehydrocholate 25克 BR，98%

改良番茄汁琼脂培养基250g/瓶用于乳酸菌总数测定incubationmedia改良番茄汁琼脂培养基250g/瓶用于乳酸菌总数测定

AcetateAgar

头孢菌素纸片(30ug/片)用于空肠弯曲菌药敏试验。

mTSB肉汤 mTSB Broth With Novobiocin 用于0157选择性增菌(ISO方法)

UBA　培养基 UBA　Medium 250 用于啤酒中厌氧菌检测

碱性蛋白胨水(APW)250g/瓶用于弧菌选择性增菌incubationmedia碱性蛋白胨水(APW)250g/瓶用于弧菌选择性增菌

RVBroth

CL-0146LTEP-a-2(人)5×106cells/瓶×2

IL27 Others Human 人 IL27 / Ierleukin-27 CHO细胞裂解液 (阳性对照)

人尿道上皮细胞裂解物HUCL

长白山猪胚胎皮肤成纤维样细胞;201184 *癌细胞，CFPAC-1细胞 Ranca细胞，小鼠细胞

SV40T转化的人胚肾细胞;293T

HA Others H1N1 甲型 H1N1 (A/Puerto Rico/8/1934) 血凝素HA1 (Hemagglutinin) 人细胞裂解液 (阳性对照)

中断位点蛋白STIL抗体说明书CL-0144LoVo(人细胞)5×106cells/瓶×2

GPT2 Others Rat 大鼠 GPT2 杆状病毒-昆虫细胞裂解液 (阳性对照)

人肾系膜细胞裂解物HRMCL

SK-OV-3[SKOV-3]细胞，人细胞 人上皮细胞,HBE细胞 脑膜细胞Many types of cells包装：5 × 105次方(1ml)

兔角膜前基质层成纤维细胞;RCFBF

TEK Others Human 人 Tie2 / CD202b 人细胞裂解液 (阳性对照)

抗体的生物素化标记实验要点：

1. 中断位点蛋白STIL抗体说明书 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及苯酚基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

抗体的鉴定：

1）中断位点蛋白STIL抗体说明书 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。