产品订购信息：
英文名称  Anti-SHANK2 

中文名称   富含脯氨酸突触相关蛋白SHANK2抗体规格 

别    名  Cortactin binding protein 1; Cortactin SH3 domain-binding protein; Cortactin-binding protein 1; CortBP1; CTTNBP1; GKAP/SAPAP interacting protein; GKAP/SAPAP-interacting protein; KIAA1022; Proline rich synapse associated protein 1; Proline-rich synapse-associated protein 1; PROSAP1; SH3 and multiple ankyrin repeat domains protein 2; SHANK; Shank2; Shank2; SPANK3; SPANK-3; SHAN2_HUMAN.

浓    度   1mg/1ml

规 格  0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Dog, Cow, Rabbit, Sheep 

产品类型   一抗  

研究领域    神经生物学 信号转导

蛋白分子量  predicted molecular weight: 159kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human SHANK2 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

富含脯氨酸突触相关蛋白SHANK2抗体规格 产品应用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 SH3 and multiple ankyrin repeat domains 1-3 (Shank1-3) of the Shank/ProSAP family are molecular scaffolds in the postsynaptic density (PSD). The PSD is an electron-dense structure underneath the postsynaptic plasma membrane of excitatory synapses that anchors and clusters glutamate receptors opposite to the presynaptic neurotransmitter release site. Shank proteins contain PDZ modular domains that coordinate the synaptic localization of ion channels, receptors, signaling enzymes, and cell adhesion molecules. The PDZ domain mediates protein-protein interactions via the recognition of a conserved sequence motif at the C-terminus of their target protein(s). Shank recruits betaPIX and PAK to spines to regulate postsynaptic structure and interacts with NMDA receptor and metabotropic glutamate receptor complexes. Transcript splice variation in the Shank family influences the spectrum of Shank-interacting proteins in the PSDs of adult and developing brain to ensure normal development.

Function : Seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors, and the actin-based cytoskeleton. May play a role in the structural and functional organization of the dendritic spine and synaptic junction.

Subunit : Interacts with CTTN/cortactin SH3 domain, LGAP1/GKAP and alpha-latrotoxin receptor 1. Is part of a complex with DLG4/PSD-95 and DLGAP1/GKAP. Interacts with GRID2, SLC9A3, CFTR and PLCB3. Interacts with DBNL (By similarity). Interacts with DNM2. Interacts with BAIAP2.

Subcellular Location : Cytoplasm. Cell junction > synapse. Cell junction > synapse > postsynaptic cell membrane; postsynaptic density. Cell projection; growth cone. Cytoplasm, postsynaptic density of neuronal cells. Colocalizes with cortactin in growth cones in differentiating hippocampal neurons.

Tissue Specificity : Isoform 3 is present in epithelial colonic cells (at protein level).

DISEASE : All isoforms except isoform 7 are expressed predominantly in brain, with highest levels in olfactory bulb, cerebral cortex, cerebellum, central gray matter and hippocampus. Moderate levels of expression are seen in the caudate putamen, thalamic nuclei and brain stem. In cerebellum primarily expressed in Purkinje cells. Isoform 7 is not expressed in brain but expressed in liver, cholangiocytes and thymus. Isoform 7 is present in pancreas, colonic mucosa and thymocytes (at protein level).

Similarity : Belongs to the SHANK family.

Contains 1 PDZ (DHR) domain.

Contains 1 SAM (sterile alpha motif) domain.

Contains 1 SH3 domain.

Database links : UniProtKB/Swiss-Prot: Q9UPX8.3

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肝浸粉 Liver Infusion 100 培养基原材料，为营养要求高的细菌提供生长营养成份

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哥伦比亚琼脂培养基2406g灭菌后冷至45℃左右时加入5%脱纤维羊血，用于空肠弯曲菌的培养。(CP、GB、EP、USP)

1601-prf OPCM-prf 少突胶质前体细胞培养基 500 ml incubation media 1601-prf OPCM-prf 少突胶质前体细胞培养基 500 ml

NutrientBroth

THBMedium

AeromonasMediumBase(Ryan)

炭疽杆菌噬菌体疽杆菌检测用配套试剂

M-ENDO agar LES 膜过滤ENDO琼脂 1.11277.0505 MERCK默克 incubation media M-ENDO agar LES 膜过滤ENDO琼脂 1.11277.0505 MERCK默克

胰蛋白胨大豆琼脂培养基 250g 用于普通的或营养要求较高的细菌的培养，还用于医药工业洁净室无菌程度的监测及消毒剂消毒效果的...

狗骨髓间质干细胞成软骨诱导分化完全培养基Thedogbonemarrowmesenchymalstemcellsintochondrocytesinduceddifferentiationofcompletemedium

3%过氧化氢酶试剂 2ml*20支 用于细菌的过氧化氢酶试验

CL-0231TE-1(人细胞)5×106cells/瓶×2

NRG1 Others Human 人 NRG1-alpha (EGF Domain) 人细胞裂解液 (阳性对照)

小鼠淋巴成纤维细胞完全培养基 100mL

MADB-106大鼠癌细胞 Breast cancer cells of MADB-106 rats 1640+10% FBS

IL1RN Protein Human 重组人 IL-1RA / IL1RN 蛋白 (Fc 标签)

Psi2 DAP(小鼠胚胎成纤维细胞) 5×106cells/瓶×2 AAV-293( 胚肾细胞)

富含脯氨酸突触相关蛋白SHANK2抗体规格CL-0228T-47D(人管癌细胞)5×106cells/瓶×2

HAVCR2 Others Mouse 小鼠 TIM-3 / HAVCR2 人细胞裂解液 (阳性对照)

人食道上皮细胞cDNAHEEpiC cDNA

LCC2细胞，人癌Tamoxifen耐药株 草鱼*细胞,L8824细胞 肝内胆管上皮细胞Many types of cells包装：5 × 105次方(1ml)

293ET(人胚肾细胞(SV40T和EBNA1基因修饰细胞)) 5×106cells/瓶×2

HVMF Pellet 人类绒毛间质成纤维细胞团块 > 1 mio.cells 人平滑肌细胞裂解物HBVSMCL

抗体的生物素化标记实验要点：

1. 富含脯氨酸突触相关蛋白SHANK2抗体规格 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及苯酚基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

抗体的鉴定：

1）富含脯氨酸突触相关蛋白SHANK2抗体规格 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。