产品订购信息：
英文名称  Anti-SPP/Signal Peptide Peptidase 

中文名称   信号肽肽酶SPP抗体规格 

别    名  Histocompatibility (minor) 13; HM 13; HM13; HM13_HUMAN; IMP 1; IMP-1; IMP1; IMPAS; IMPAS-1; Intramembrane Protease 1; Intramembrane protease; Minor Histocompatibility 13; Minor histocompatibility antigen 13; Minor histocompatibility antigen H13; MSTP086; Presenilin like protein 3; Presenilin-like protein 3; PSENL 3; PSENL3; PSL 3; PSL3; Signal peptide peptidase; Signal peptide peptidase beta; SPP; dJ324O17.1; H13; hIMP1; hIMP1 protein.

浓    度   1mg/1ml

规 格  0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit 

产品类型   一抗  

研究领域     免疫学 神经生物学 信号转导

蛋白分子量  predicted molecular weight: 42kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human Signal Peptide Peptidase 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

信号肽肽酶SPP抗体规格 产品应用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 The endoplasmic reticulum exerts a quality control over newly synthesized proteins and a variety of components have been implicated in the specific recognition of aberrant or misfolded polypeptides. Signal peptide peptidase (SPP) catalyzes intramembrane proteolysis of some signal peptides after they have been cleaved from a preprotein, resulting in the release of the fragment from the ER membrane into the cytoplasm. SPP is required to generate lymphocyte cell surface (HLA-E) epitopes derived from MHC class I signal peptides, and may play a role in graft rejection. It also may be necessary for the removal of the signal peptide that remains attached to the hepatitis C virus core protein after the initial proteolytic processing of the polyprotein.

Function : Catalyzes intramembrane proteolysis of some signal peptides after they have been cleaved from a preprotein, resulting in the release of the fragment from the ER membrane into the cytoplasm. Required to generate lymphocyte cell surface (HLA-E) epitopes derived from MHC class I signal peptides. May play a role in graft rejection. May be necessary for the removal of the signal peptide that remains attached to the hepatitis C virus core protein after the initial proteolytic processing of the polyprotein. Involved in the intramembrane cleavage of the integral membrane protein PSEN1.

Subcellular Location : Endoplasmic reticulum membrane; Multi-pass membrane protein. Isoform 4: Cell membrane; Multi-pass membrane protein.

Tissue Specificity : Widely expressed with highest levels in kidney, liver, placenta, lung, leukocytes and small intestine and reduced expression in heart and skeletal muscle. Expressed abundantly in the CNS with highest levels in thalamus and medulla.

Post-translational modifications : N-glycosylated.

Similarity : Belongs to the peptidase A22B family.

哥伦比亚琼脂培养基250g用于梭菌分离培养

麦芽浸膏汤 200(g) incubation media 麦芽浸膏汤 200(g)

念珠菌显色培养基 1000ml/瓶 用于念珠菌特别是白色念珠菌的选择性分离和初步鉴别

泛酸测定培养基250用于*食品和乳粉中泛酸测定incubationmedia泛酸测定培养基250用于*食品和乳粉中泛酸测定

PolyPeptoneYeastExtractMedium

哥伦比亚血琼脂基础250g营养非常丰富，用于各种细菌的基础培养基

CZAPEK-DOX agar 查贝克-多克斯琼脂 1.05460.0500 MERCK默克 incubation media CZAPEK-DOX agar 查贝克-多克斯琼脂 1.05460.0500 MERCK默克

万古霉素(改良月桂基盐胰蛋白胨肉汤冻干配套试剂) 10支/盒 每支添加于100ml(022212)中配成MLST

胎儿骨髓间质干细胞成脂诱导分化培养基Fetalbonemarrowmesenchymalstemcellsintotheculturemediuminduceddifferentiationoffat

FraserSupplement

尿素酶琼脂基础250g生化培养基，用于细菌脲酶检测(GB、SN标准)

Mediaforisolationofmagnetotacticbacteria

乳糖蛋白胨培养液 Lactose Peptone Broth 250 用于饮用水，水源水中总大肠菌群的测定(GB标准)

HB培养基250g/瓶用于植物组织培养incubationmediaHB培养基250g/瓶用于植物组织培养

FB1(Half-Fraser)添加剂(A、B) 2ml/支*40 每支添加到225ml HBJ006中

CL-0247ZR-75-1(人癌细胞)5×106cells/瓶×2

NRG1 Others Human 人 NRG1-alpha (ECD) 人细胞裂解液 (阳性对照)

小鼠甲状腺上皮细胞完全培养基 100mL

Sf9昆虫卵巢细胞 Sf9 insect ovarian cells GPM-115无血清无蛋白昆虫细胞悬浮培养基(金普诺安 Cat#GPM001L01)

IL1A Protein Human 重组人 IL-1 alpha / IL1A / IL1F1 蛋白

PC-3M-IE8(人高转移细胞株) 5×106cells/瓶×2 Lec1(卵巢细胞)

信号肽肽酶SPP抗体规格CL-0244WISH(人羊膜细胞)5×106cells/瓶×2

SRGN Others Human 人 Serglycin / SRGN 人细胞裂解液 (阳性对照)

人骨骼肌细胞cDNAHSkMC cDNA

4T1细胞，人癌细胞 昆虫细胞系,Sf-21细胞 肺微内皮细胞Many types of cells包装：5 × 105次方(1ml)

Y3-Ag 1.2.3(大鼠细胞) 5×106cells/瓶×2

NHDF adult Pellet 正常人皮肤成纤维细胞团块(捐献者) > 1 mio.cells CCCSCK

抗体的生物素化标记实验要点：

1. 信号肽肽酶SPP抗体规格 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及苯酚基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

抗体的鉴定：

1）信号肽肽酶SPP抗体规格 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。