全、新、优、品、好四大特点：

泛素连接酶Siah1抗体费用 全：公司提供上万种产品，涵盖了生物试剂，elisa试剂盒，标准品，培养基，原装耗材，抗体、培养基、ATCC细胞等，基本上各种科研所需产品在我司都能找到。

新：产品更新速度较快，基本上每周都有新产品出现。

优：产品质量好，投诉比较少。

好：我公司具有优质的技术团队，产品一旦售出，实验过程中遇到困难可提供在线技术咨询。使您使用产品时没有任何的后顾之忧。

泛素连接酶Siah1抗体费用 英文名称  Anti-SIAH1

中文名称  泛素连接酶Siah1抗体费用 

别    名 hSIAH1; HUMSIAH; Seven in absentia homolog 1 (Drosophila); Seven in absentia homolog 1; Siah 1; Siah 1a; Ubiquitin ligase SIAH1; E3 ubiquitin-protein ligase SIAH1; Seven in absentia homolog 1; Siah-1; Siah-1a.

产品属性：

浓    度  1mg/1ml

规 格  0.1ml/100μg 0.2ml/200μg

抗体来源  Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit

产品类型   一抗  

研究领域     免疫学 信号转导 转录调节因子 激酶和磷酸酶 泛素

蛋白分子量  predicted molecular weight: 34kDa 

性    状   Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from human SIAH1

亚    型  IgG

纯化方法   affinity purified by Protein A

储 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

泛素连接酶Siah1抗体费用 产品应用   WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 This gene encodes a protein that is a member of the seven in absentia homolog (SIAH) family. The protein is an E3 ligase and is involved in ubiquitination and proteasome-mediated degradation of specific proteins. The activity of this ubiquitin ligase has been implicated in the development of certain forms of Parkinson's disease, the regulation of the cellular response to hypoxia and induction of apoptosis. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized.

Function : E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates E3 ubiquitin ligase activity either through direct binding to substrates or by functioning as the essential RING domain subunit of larger E3 complexes. Triggers the ubiquitin-mediated degradation of many substrates, including proteins involved in transcription regulation (MYB, POU2AF1, PML and RBBP8), a cell surface receptor (DCC), the cell-surface receptor-type tyrosine kinase FLT3, the cytoplasmic signal transduction molecules (KLF10/TIEG1 and NUMB), an antiapoptotic protein (BAG1), a microtubule motor protein (KIF22), a protein involved in synaptic vesicle function in neurons (SYP), a structural protein (CTNNB1) and SNCAIP. Confers constitutive instability to HIPK2 through proteasomal degradation. It is thereby involved in many cellular processes such as apoptosis, tumor suppression, cell cycle, axon guidance, transcription regulation, spermatogenesis and TNF-alpha signaling. Has some overlapping function with SIAH2. Induces apoptosis in cooperation with PEG3. Upon nitric oxid (NO) generation that follows apoptotic stimulation, interacts with S-nitrosylated GAPDH, mediating the translocation of GAPDH to the nucleus. GAPDH acts as a stabilizer of SIAH1, facilitating the degradation of nuclear proteins.

Subcellular Location : Cytoplasm. Nucleus. Predominantly cytoplasmic. Partially nuclear.

Tissue Specificity : Widely expressed at a low level. Down-regulated in advanced hepatocellular carcinomas.

Post-translational modifications : Phosphorylated on Ser-19 by ATM and ATR. This phosphorylation disrupts SIAH1 interaction with HIPK2, and subsequent proteasomal degradation of HIPK2.

Similarity : Belongs to the SINA (Seven in absentia) family.

Contains 1 RING-type zinc finger.

Contains 1 SIAH-type zinc finger.

实验流程：

正在热销的相关产品：

改良月桂基盐胰蛋白月示肉汤(MLST)2220g用于奶粉中阪崎肠杆菌的检验的增菌和分子生物学检验方法(PCR)选择性增菌

弯曲菌无血选择琼脂基础 (CM0739) Oxoid incubation media 弯曲菌无血选择琼脂基础 (CM0739) Oxoid

威尔酵母 药用菌 支/瓶

MEE肉汤250g用于肠道菌的选择性增菌培养(SN标准)

十二烷基琼脂 250g 用于杆菌抗噬菌体培养

甘露醇发酵培养基24030250g用于鉴别金黄色葡萄球菌发酵甘露醇试验(化妆品卫生规范微生物检验方法2007版)。

Lambda Broth 培养基 100g incubation media Lambda Broth 培养基 100g

芽孢杆菌 支/瓶

葡萄糖肉汤250g用于营养苛求菌培养和肠道菌产气实验

无淀粉改良罗氏培养基 250g 用于结核杆菌分离培养

BCYEAgarBase

MRS agar Lactobacillus agar acc.to DEMAN,ROGOSA and SHARPE MRS 1.10660.0500 MERCK默克 incubation media MRS agar Lactobacillus agar acc.to DEMAN,ROGOSA and SHARPE MRS 1.10660.0500 MERCK默克

叠氮化物葡萄糖液态培养基 250g 用于水和污水中粪性链球菌发酵法的推测试验(CJ/T148-2001和SN/T2206.5-2009)。

SD大鼠骨髓间质干细胞完全培养基SDratbonemarrowmesenchymalstemcellsincompletemedium

MUGNutrientAgar

CL-0184PANC-1(人*癌细胞)5×106cells/瓶×2

TNK2 Others Human 人 ACK1 / TNK2 杆状病毒-昆虫细胞裂解液 (阳性对照)

人上皮细胞裂解物HMEpiCL

HCC1428细胞，人癌细胞 人小细胞细胞,LTEP-P细胞 晶状体上皮细胞Many types of cells包装：5 × 105次方(1ml)

狗肺成纤维样细胞;DogL1

TNFRSF4 Others Human 人 TNFRSF4 / CD134 人细胞裂解液 (阳性对照)

泛素连接酶Siah1抗体费用CL-0183PA317(小鼠成纤维细胞)5×106cells/瓶×2

SELP Others Human 人 SELP / selectin P / P-selectin 人细胞裂解液 (阳性对照)

小鼠肠粘膜上皮细胞完全培养基 100mL

3D4/21猪肺泡巨噬细胞 Porcine alveolar macrophage 3D4/21 1640+10% FBS+0.1mM NEAA+1mM 酸

CF Protein Mouse 重组小鼠 CF / Ciliary Neuroophic Factor 蛋白 (His 标签)

RBL-1(大鼠嗜碱性粒细胞性细胞) 5×106cells/瓶×2 PC-3(人细胞)

技术外包服务:

★泛素连接酶Siah1抗体费用 分子生物学：质粒抽提、PCR、Q-PCR、RT-PCR、分子生物学：基因合成、引物合成、基因测序、载体构建等

★蛋白工程：原核、哺乳动物蛋白表达系统等

★病毒包装：腺病毒、慢病毒等

★抗体工程：磁珠分选、病理染色、WB、ELISA、IP、IF、IHC、FACS、Confocal等等

★细胞工程：细胞表型分析(凋亡、增殖、周期、迁移、侵袭、修复、克隆形成)、细胞培养、细胞膜制备、稳定细胞株构建、细胞RNAi技术等等。