产品订购信息：
英文名称  Anti-SORCS1 

中文名称   液泡蛋白分选受体SORCS抗体规格 

别    名  SorCS 1; SORCS; SORCS-1; SORCS receptor 1; Sortilin related VPS10 domain containing receptor 1; VPS10 domain containing receptor SorCS1; VPS10 domain receptor protein SORCS 1; VPS10 domain receptor protein SORCS; VPS10 domain receptor protein SORCS1; VPS10 domain receptor SorCS; FLJ41758; FLJ43475; FLJ44957; hSorCS; OTTHUMP00000058855; SORC1_HUMAN.

浓    度   1mg/1ml

规 格  0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep 

产品类型   一抗  

研究领域    细胞生物 神经生物学 细胞类型标志物 Alzheimer's

蛋白分子量  predicted molecular weight: 127kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human SORCS1 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

液泡蛋白分选受体SORCS抗体规格 产品应用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 There are three sorCS genes that have diverse, partially overlapping functions in the central nervous system. In the developing and mature central nervous system, the homologous SorCS1 and SorCS2 genes and the SorCS3 gene are expressed in a combinatorial, non-overlapping pattern. SorCS proteins show homology to the mosaic receptor SorLA and the neurotensin receptor sortilin, based on a common VPS10 domain, which is the hallmark of the SorCS receptor family. SorCS1 is a type 1 receptor containing a VPS10P domain and a leucine-rich domain. Alternative splicing of human SorCS1 results in four isoforms with different cytoplasmic tails and differential expression in tissues. Human SorCS1 is detected in fetal and infant brain and in fetal retina. Alternative splicing of murine SorCS1 also results in four isoforms. Murine isoform 1 is highly expressed in brain and at lower levels in heart, liver and kidney. It is detected in newborn mouse brain and in adult olfactory bulb and cerebral cortex. Murine isoform 2 is highly expressed in liver and at lower levels in heart, brain, kidney and testis.

Function : SorCS1 belongs to the family of VPS10 domain-containing receptors (the name is derived from the yeast vacuolar protein sorting protein-10, which is involved in sorting of carboxy-peptidase Y from the Golgi apparatus to the vacuole). SorCS1 immunoreactivity is widespread in a population of neurons throughout the brain. Two different types of cellular localization were observed. Most SorCS1 immunoreactive neurons exhibit a punctate cytoplasmic staining which extends into the dendrites, whilst occassionally SorCS1 neuronal immunoreactivity is associated with the plasma membrane.

Subcellular Location : Membrane; Single-pass type I membrane protein.

Tissue Specificity : Detected in fetal and infant brain and in fetal retina.

Post-translational modifications : O-glycosylated.

Similarity : Belongs to the VPS10-related sortilin family. SORCS subfamily.

Contains 5 BNR repeats.

Contains 1 PKD domain.

Database links : UniProtKB/Swiss-Prot: Q8WY21.3

肝肉琼脂培养基250g用于亚盐还原厌氧孢子的计数

L型细菌高渗盐增菌培养基 250g 用于L型细菌增菌培养

LBS 琼脂 LBS　agar

含0.6%酵母浸膏的胰酪胨大豆肉汤(TSB-YE)250g/瓶用于李斯特氏菌的培养incubationmedia含0.6%酵母浸膏的胰酪胨大豆肉汤(TSB-YE)250g/瓶用于李斯特氏菌的培养

瓷珠菌种保存管(20支) 20支/盒 用于菌种保存(瓷珠法)

高渗真菌学琼脂250g用于高渗食品中酵母菌和霉菌检测

L型细菌高渗盐增菌培养基 250(g) incubation media L型细菌高渗盐增菌培养基 250(g)

SOB肉汤 SOB Broth 250克 BR

改良Y培养基250用于分离小肠耶尔森氏菌(GB标准)incubationmedia改良Y培养基250用于分离小肠耶尔森氏菌(GB标准)

PlateCountAgar

溴化十六烷基胺琼脂250g用于绿脓假单胞菌的分离培养(新药典)

MH琼脂(MHA) 250(g) incubation media MH琼脂(MHA) 250(g)

四酸煌绿增菌液 2X5支 含碘液2ml/支和煌绿1ml/支，2支添加于100mL(023030)中配成TTB

三糖铁琼脂培养基TripleSugarIronAgarMedium用于肠杆菌科细菌的菌的生化反应筛选。

PhosphateSalineBuffer,Modified

CL-0215SK-OV-3(人细胞)5×106cells/瓶×2

APOM Others Human 人 APOM 人细胞裂解液 (阳性对照)

小鼠骨髓基质细胞完全培养基 100mL

PK(15)猪肾上皮细胞 PK (15) of porcine kidney epithelial cells MEM+10% FBS

IL18BP Protein Human 重组人 IL18BPa 蛋白 (His 标签)

QGY-7703(人细胞) 5×106cells/瓶×2 7721(7721细胞)

液泡蛋白分选受体SORCS抗体规格CL-0212SK-Hep-1(人细胞)5×106cells/瓶×2

CREG1 Others Mouse 小鼠 CREG / CREG1 人细胞裂解液 (阳性对照)

人表皮角质细胞-cDNAHEK-acDNA

MCF/Adr细胞，人癌阿霉素耐药细胞株 团头鲂尾鳍细胞,WCF细胞 骨细胞Many types of cells包装：5 × 105次方(1ml)

2V6.11(人胚肾细胞) 5×106cells/瓶×2

HCF Pellet 人类成纤维细胞团块 > 1 mio.cells 人海马趾星形胶质细胞裂解物HA-h L

抗体的生物素化标记实验要点：

1. 液泡蛋白分选受体SORCS抗体规格 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及苯酚基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

抗体的鉴定：

1）液泡蛋白分选受体SORCS抗体规格 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。