抗体的生物素化标记实验要点：

1. S期激酶相关蛋白1抗体说明书 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及苯酚基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

产品订购信息：
英文名称  Anti-SKP1 

中文名称   S期激酶相关蛋白1抗体说明书 

别    名  Cyclin A/CDK2 associated p19; Cyclin A/CDK2 associated protein p19; Cyclin-A/CDK2-associated protein p19; EMC 19; EMC19; OCP 2; OCP II; OCP II protein; OCP-2; OCP-II; OCP2; OCPII; organ of Corti protein 2; Organ of Corti protein II; p19A; p19skp1; RNA polymerase II elongation factor like protein; RNA polymerase II elongation factor like protein OCP2; RNA polymerase II elongation factor-like protein; S phase kinase associated protein 1; S phase kinase associated protein 1A; S phase kinase associated protein 1A p19A; S-phase kinase-associated protein 1; SIII; Skp 1; SKP 1A; skp1; SKP1_HUMAN; SKP1A; TCEB1L; Transcription elongation factor B (SIII) polypeptide 1 like; Transcription Elongation Factor B 1 like; Transcription Elongation Factor B 1-like; Transcription elongation factor B; Transcription elongation factor B SIII polypeptide 1 like.

浓    度   1mg/1ml

规 格  0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Sheep 

产品类型   一抗  

研究领域    细胞生物 免疫学

蛋白分子量  predicted molecular weight: 19kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human SKP1 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

S期激酶相关蛋白1抗体说明书 产品应用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 Essential component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, serves as an adapter that links the F-box protein to CUL1. SCF(BTRC) mediates the ubiquitination of NFKBIA at 'Lys-21' and 'Lys-22'; the degradation frees the associated NFKB1-RELA dimer to translocate into the nucleus and to activate transcription. SCF(Cyclin F) directs ubiquitination of CP110.

Function : Essential component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, serves as an adapter that links the F-box protein to CUL1. SCF(BTRC) mediates the ubiquitination of NFKBIA at 'Lys-21' and 'Lys-22'; the degradation frees the associated NFKB1-RELA dimer to translocate into the nucleus and to activate transcription. SCF(Cyclin F) directs ubiquitination of CP110.

Subunit : Component of an E3 ubiquitin ligase complex containing UBE2D1, SIAH1, CACYBP/SIP, SKP1, APC and TBL1X. Component of the SCF(BTRC) complex, composed of SKP1, CUL1 and BTRC. Part of a SCF(BTRC)-like complex lacking CUL1, which is associated with phosphorylated NFKBIA and RELA; RELA interacts directly with NFKBIA. Component of the SCF(FBXO44) complex, composed of SKP1, CUL1 and FBXO44. Identified in a complex with SKP2, CKS1B and CDKN1B. Interacts with the cyclin A/CDK2 complex. Part of a SCF-like complex consisting of CUL7, RBX1, SKP1 and FBXW8. Part of several SCF complexes containing SKP1 and one of the F-box proteins. Component of a SCF(SKP2)-like complex containing CUL1, SKP1, TRIM21 and SKP2. Interacts with FBXO2, FBXO4, FBXW7 and TRIM21. Interacts with FBXO45 (By similarity). Component of the SCF(FBXO17) complex, composed of SKP1, CUL1 and FBXO17. Component of the SCF(FBXO27) complex, composed of SKP1, CUL1 and FBXO27. Component of the SCF(Cyclin F) complex consisting of CUL1, RBX1, SKP1 and CCNF.

Similarity : Belongs to the SKP1 family.

Database links : UniProtKB/Swiss-Prot: P63208.2

抗体的鉴定：

1）S期激酶相关蛋白1抗体说明书 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。

微管关联蛋白2 单克隆抗体  MAP2 Monoclonal Antibody  700/30ul#1540/100ul#2520/200ul

CD1 单克隆抗体  CD1 Monoclonal Antibody  700/30ul#1540/100ul#2520/200ul

CD2 单克隆抗体  CD2 Monoclonal Antibody  700/30ul#1540/100ul#2520/200ul

CD4 单克隆抗体  CD4 Monoclonal Antibody  700/30ul#1540/100ul#2520/200ul

CD5 单克隆抗体  CD5 Monoclonal Antibody  700/30ul#1540/100ul#2520/200ul

易感基因环状结构域蛋白抗体     抑制素结合蛋白抗体

相关抗原BRCAA1抗体     抑制素βC抗体

骨髓基质干细胞抗原1抗体     抑制素βB/Inhibin β B抗体

蛋白酪氨酸激酶BAP135抗体     抑制素α/Inhibin α抗体

磷酸化促凋亡Bik蛋白抗体     抑制素A/Inhibin βA抗体

小泡相关膜蛋白7 多克隆抗体  VAMP7 Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

神经管畸形相关蛋白 多克隆抗体  VANG2 Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

缝隙样蛋白 多克隆抗体  VASN Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

小泡胺运输蛋白1同源物 多克隆抗体  VAT1 Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

ATP酶H+转运溶酶体70kDa,V1亚基A 多克隆抗体  VATA Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

地黄 Rehmannia glinosa Libosch. RhMannioside D 地黄苷D 81720-08-3 C27H42O20 ≥98%

苦玄参苷IAPicfqltcrrcqninIcHPLC≥98%，20mg/支

β-桉叶醇;Β-桉叶醇 β-Eudesmol 51317-08-9 20mg HPLC≥98% 用于含量测定

中药对照药材桃金娘果121471-200401TLC法鉴别

DECURSIN紫花前胡素纯度：≥97%

S期激酶相关蛋白1抗体说明书大豆Glycinemax(L.)merr Soyasaponin Ba 大豆皂苷Ba 114590-20-4 C48H78O19 ≥98%

原薯蓣皂苷ProtodioscinHPLC≥98%，20mg/支

远志皂苷A Onjisaponin A 20mg HPLC≥98% 用于含量测定

对照品新霉胺130411-200307检查

Cyanidin chloride录化花青素纯度：>98%