产品订购信息：
英文名称  Anti-SRRM1 

中文名称   丝氨酸/精氨酸相关核基质蛋白1抗体规格 

别    名  Ser/Arg related nuclear matrix protein; Ser/Arg-related nuclear matrix protein; Serine/arginine repetitive matrix protein 1; SR related nuclear matrix protein of 160 kDa; SR-related nuclear matrix protein of 160 kDa; SRm160; Srrm1; SRRM1_HUMAN.

浓    度   1mg/1ml

规 格  0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit 

产品类型   一抗  

研究领域    细胞生物 染色质和核信号 表观遗传学

蛋白分子量  predicted molecular weight: 102kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human SRRM1 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

丝氨酸/精氨酸相关核基质蛋白1抗体规格 产品应用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 Part of pre- and post-splicing multiprotein mRNP complexes. Involved in numerous pre-mRNA processing events. Promotes constitutive and exonic splicing enhancer (ESE)-dependent splicing activation by bridging together sequence-specific (SR family proteins, SFRS4, SFRS5 and TRA2B/SFRS10) and basal snRNP (SNRP70 and SNRPA1) factors of the spliceosome. Stimulates mRNA 3'-end cleavage independently of the formation of an exon junction complex. Binds both pre-mRNA and spliced mRNA 20-25 nt upstream of exon-exon junctions. Binds RNA and DNA with low sequence specificity and has similar preference for either double- or single-stranded nucleic acid substrates.

Function : Part of pre- and post-splicing multiprotein mRNPcomplexes. Involved in numerous pre-mRNA processing events.Promotes constitutive and exonic splicing enhancer (ESE)-dependentsplicing activation by bridging together sequence-specific (SRfamily proteins, SFRS4, SFRS5 and TRA2B/SFRS10) and basal snRNP(SNRP70 and SNRPA1) factors of the spliceosome. Stimulates mRNA3'-end cleavage independently of the formation of an exon junctioncomplex. Binds both pre-mRNA and spliced mRNA 20-25 nt upstream ofexon-exon junctions. Binds RNA and DNA with low sequencespecificity and has similar preference for either double- orsingle-stranded nucleic acid substrates.

Subunit : Identified in the spliceosome C complex. Found in apre-mRNA splicing complex with SFRS4, SFRS5, SNRP70, SNRPA1, SRRM1and SRRM2. Found in a pre-mRNA exonic splicing enhancer (ESE)complex with SNRP70, SNRPA1, SRRM1 and TRA2B/SFRS10. Found in amRNA splicing-dependent exon junction complex (EJC) with DEK,PRPF8, NCBP1, RBM8A, RNPS1, SRRM1 and ALYREF/THOC4. Interacts withBAT1, CPSF1, RBM8A, RNPS1, and ALYREF/THOC4. Seems to be a compoundof RNA export complexes that are released from speckles in aATP-dependent manner.

Subcellular Location : Nucleus matrix. Nucleus speckle.

Similarity : Belongs to the splicing factor SR family.

Contains 1 PWI domain.

Database links : UniProtKB/Swiss-Prot: Q8IYB3.2

转录辅助因子退变样蛋白4 多克隆抗体  VGLL4 Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

溶质载体家族17(依赖无机磷酸协同转运蛋白)成员6 多克隆抗体  VGLU2 Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

vonHippel-Lindau抑制因子 多克隆抗体  VHL Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

极受体 多克隆抗体  VLDLR Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

波形蛋白IF关联卷曲螺旋蛋白 多克隆抗体  VMAC Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

自噬相关蛋白12抗体     孕激素受体抗体

自噬相关蛋白13抗体     孕激素受体β（MPRβ）抗体

自噬相关蛋白3抗体     阅读障碍相关蛋白DLX2抗体

G1氨基A型受体相似蛋白2抗体     远端上游元件结合蛋白3抗体

整合素样金属蛋白酶与凝血酶4型抗体     原纤维蛋白2抗体

新朊蛋白抗体     CWF19L1蛋白抗体

SHISA蛋白家族9抗体     CWC22蛋白抗体

固醇携带蛋白2抗体     CUTL1蛋白抗体

唾液酸结合性免疫球蛋白样凝集素1抗体     CULLIN抗体

SPOP蛋白抗体     Cullin3抗体

棉籽Cotton seed Raffinose 棉子糖 512-69-6 C18H32O16 ≥98%

川陈皮速NobilqtinHPLC≥98%;20mg/支

7-去甲基银杏双黄同;7-去甲基银杏双黄同;果素 7-去甲基银杏双黄同 Bilobetin 521-32-4 20mg HPLC≥98% 用于含量测定

中药对照药材雄蚕蛾(家蚕)121457-200501TLC法鉴别

Ginkgolic Acid (C15:1)银杏酸(C15:1)纯度：≥99%

丝氨酸/精氨酸相关核基质蛋白1抗体规格云南红豆杉Taxus yunnanensis Taxiresinol 紫杉脂素 40951-69-7 C19H22O6 ≥97%

月桂氮卓同Lcuroccprcm297-89-30.1ml

芦荟苷;芦荟甙;芦荟大黄素甙;芦荟素 Aloin 1415-73-2 20mg HPLC≥98% 用于含量测定

对照品他唑巴坦130511-200402含量测定

大花双参苷A iplostoside A 20mg HPLC≥98% 大花双参苷A用于含量测定

抗体的生物素化标记实验要点：

1. 丝氨酸/精氨酸相关核基质蛋白1抗体规格 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及苯酚基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

抗体的鉴定：

1）丝氨酸/精氨酸相关核基质蛋白1抗体规格 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。