抗体的生物素化标记实验要点：

1. 长链脂肪酸转运蛋白4抗体规格 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及苯酚基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

产品订购信息：
英文名称  Anti-SLC27A4/FATP4 

中文名称   长链脂肪酸转运蛋白4抗体规格 

别    名  ACSVL 4; ACSVL-4; ACSVL4; EC 6.2.1; FATP 4; FATP4; Fatty acid transport protein 4; Fatty acid transport protein4; IPS; Long chain fatty acid transport protein 4; Long chain fatty acid transport protein4; OTTHUMP00000022264; S27A4; SLC27 A4; SLC27A 4; Solute carrier family 27 (fatty acid transporter) member 4; Solute carrier family 27 member 4; Solute carrier family 27 member4; S27A4_HUMAN.

浓    度   1mg/1ml

规 格  0.1ml/100μg 0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Chicken, Pig, Cow, Horse 

产品类型   一抗  

研究领域     心 神经生物学 信号转导

蛋白分子量  predicted molecular weight: 72kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human SLC27A4 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

长链脂肪酸转运蛋白4抗体规格 产品应用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 SLC27A4 / FATP4 plays a role in the transport of long chain fatty acids across the plasma membrane. It has acyl-coA ligase activity for long chain and very long chain fatty acids. Deletion of the SLC27A4 gene results in embryonic lethality, which is attributed to the need for fat absorption across the visceral endoderm early in embryonic development. Expression of FAT4P in the intestinal lining is thought to be altered in response to dietary fat.

Function : nvolved in translocation of long-chain fatty acids (LFCA) across the plasma membrane. Appears to be the principal fatty acid transporter in small intestinal enterocytes. Plays a role in the formation of the epidermal barrier. Required for fat absorption in early embryogenesis. Has acyl-CoA ligase activity for long-chain and very-long-chain fatty acids

Subcellular Location : Membrane; Multi-pass membrane protein

Tissue Specificity : Expressed at highest levels in brain, testis, colon and kidney. Expressed at medium levels in heart and liver, small intestine and stomach. Expressed at low levels in peripheral leukocytes, bone marrow, skeletal muscle and aorta. Expressed in adipose tissue.

DISEASE : Defects in SLC27A4 are the cause of ichthyosis prematurity syndrome (IPS) [MIM:608649]. A keratinization disorder characterized by complications in the second trimester of pregnancy resulting from polyhydramnion, with premature birth of a child with thick caseous desquamating epidermis, respiratory complications and transient eosinophilia. After recovery during the first months of life, the symptoms are relatively benign and the patients suffer from a lifelong non-scaly ichthyosis with atopic manifestations.

Similarity : Belongs to the ATP-dependent AMP-binding enzyme family.

抗体的鉴定：

1）长链脂肪酸转运蛋白4抗体规格 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。

TNF受体关联因子7 多克隆抗体  TRAF7 Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

驱动蛋白结合蛋白1 多克隆抗体  TRAK1 Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

髓细胞触发受体2 多克隆抗体  TREM2 Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

黑素转铁蛋白 多克隆抗体  TRFM Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

含三联基元蛋白23 多克隆抗体  TRI23 Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

抗激素/升压素/加压素/加压素抗体     整合素αE抗体Integrin αE

ATP结合盒蛋白家族GCN20F家族1抗体     整合素α7抗体

激素受体相关蛋白16抗体     整合素α6抗体

β淀粉样肽1-40 C端/Aβ1-40 C端抗体     整合素α5抗体Integrin α5

含锚蛋白重复序列-细胞因子信号抑制物盒蛋白家族13抗体     整合素α4抗体

血小板衍生生长因子受体β (phospho Tyr1021) 多克隆抗体  PDGFR-β (phospho Tyr1021) Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

真核翻译启动因子 2-alpha激酶(phospho Thr981) 多克隆抗体  PERK (phospho Thr981) Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

fms样酪氨酸激酶1(phospho Tyr1048) 多克隆抗体  Flt-1 (phospho Tyr1048) Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

突触核蛋白α (phospho Tyr125) 多克隆抗体  Synuclein-α (phospho Tyr125) Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

ERK 1/2 (phospho Thr202/Y204) 多克隆抗体  ERK 1/2 (phospho Thr202/Y204) Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

石榴Punica granatum L. Punicalin 石榴皮鞣素 65995-64-4 C34H22O22 ≥98%

花椒毒速XcnthotoxinHPLC≥98%，20mg/支

豆甾醇;豆固醇 Stigmasterol 201-482-7 20mg HPLC≥98% 用于含量测定

中药对照药材胡椒(黑胡椒)121440-200401TLC法鉴别

cedrol,cedar camphor,cypress camphor雪松醇纯度：≥97%

长链脂肪酸转运蛋白4抗体规格轻臭槟榔碱 Arecoline xy7nobromide 分 子 量：236.1100 CAS编号：300-08-3 分子式：C8H14BrNO2 别　　名：槟榔碱轻臭盐;轻臭槟榔碱;轻臭丙胺 基本用途：含量测定。

云南含笑Michelia yunnanensis Reynosin 喘诺木烯内酯 28254-53-7 C15H20O3 模拟天然水铜溶液成分分析标准物质-铜080 97

中药对照药材鱼腥草121046-200403TLC法鉴别

Eleheroside B刺五加苷B纯度：95%

余甘子Phyllanthus emblica Lup-20(29)-ene-3α,23-diol 20(29)-扇豆烯-3,23-二醇 32451-85-7 C30H50O2 ≥98.5%