产品订购信息：
英文名称  Anti-Synaptojanin/INPP5G 

中文名称   磷酸肌醇磷酸酶蛋白INPP5G抗体品牌 

别    名  5-trisphosphate 5-phosphatase 1; Inositol 5' phosphatase; INPP 5G; INPP5G; KIAA0910; Polyphosphoinositide phosphatase; Synaptic inositol 1 4 5 trisphosphate 5 phosphatase 1; Synaptic inositol 145 trisphosphate 5 phosphatase 1; Synaptic inositol-1 antibody Synaptic inositol-145-trisphosphate 5-phosphatase 1; Synaptojanin 1; Synaptojanin 1 polyphosphoinositide phosphatase; Synaptojanin-1; Synaptojanin1; Synj 1; Synj1; SYNJ1_HUMAN.

浓    度   1mg/1ml

规 格  0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep 

产品类型   一抗  

研究领域    细胞生物 神经生物学 信号转导

蛋白分子量  predicted molecular weight: 173kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human Synaptojanin/INPP5G 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

磷酸肌醇磷酸酶蛋白INPP5G抗体品牌 产品应用    ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 The inositol polyphosphate 5-phosphatases selectively remove the phosphate from the 5-position of various phosphatidylinositols, which generate second messengers in response to extracellular signals. Synaptojanins are characterized by an N-terminal SAC1-like sequence, a central 5-phosphate domain, and a unique C-terminal sequence and have been shown to use phosphatidylinositol 4,5-bisphosphate as a substrate. Synaptojanins exist as two isoforms, synaptojanin 1 and 2, which differ in the C-terminal domain, and each isoform has multiple variants produced by alternative splicing. Synaptojanin 1 is expressed as two major forms: the shorter is found in brain while the longer is expressed in peripheral tissues. Eight splice variants of synaptojanin 2 have been detected, including a brain specific isoform. Synaptojanins are thought to participate in the endocytosis of synaptic vesicles and the regulation of the actin cytoskeleton.

Function : Inositol 5-phosphatase which has a role in clathrin-mediated endocytosis.

Subunit : Interacts with ASH/GRB2. Interacts with PACSIN1, PACSIN2 and PACSIN3 (By similarity). Binds AMPH, SH3GL1, SH3GL2 and SH3GL3. Interacts with MYO1E (via SH3 domain).

Subcellular Location : Cytoplasm.

Tissue Specificity : Concentrated at clathrin-coated endocytic intermediates in nerve terminals. Isoform 1 is more enriched than isoform 2 in developing brain as well as non-neuronal cells. Isoform 2 is very abundant in nerve terminals.

Similarity : Belongs to the synaptojanin family.

In the central section; belongs to the inositol-1,4,5-trisphosphate 5-phosphatase family.

Contains 1 RRM (RNA recognition motif) domain.

Contains 1 SAC domain.

Database links : UniProtKB/Swiss-Prot: O43426.2

增生型蛋白1 多克隆抗体  TEFF2 Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

肌联蛋白帽 多克隆抗体  TELT Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

肌腱蛋白R 多克隆抗体  Tenascin-R Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

腱生蛋白N 多克隆抗体  TENN Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

端粒重复结合因子2 多克隆抗体  TERF2 Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

酸性磷酸酶样蛋白2抗体     致癌基因n-Myc抗体

AHNAK核蛋白2抗体     致癌基因C-Myc抗体

二磷酸腺苷核糖基化因子6相互作用蛋白抗体     质子梯度调节蛋白1抗体

腺苷酸激酶1抗体     酯酶抑制蛋白C1IN抗体

顶体前体蛋白抗体     指状蛋白RET抗体

荧光素酶 小鼠单克隆抗体(9D6)  Luciferase Mouse Monoclonal Antibody(9D6)  700/30ul#1540/100ul#2520/200ul

磷酸酯酶与张力蛋白同源物 小鼠单克隆抗体(2C10)  PTEN Mouse Monoclonal Antibody(2C10)  700/30ul#1540/100ul#2520/200ul

磷酸酯酶与张力蛋白同源物 小鼠单克隆抗体(9E8)  PTEN Mouse Monoclonal Antibody(9E8)  700/30ul#1540/100ul#2520/200ul

细胞周期蛋白B1 小鼠单克隆抗体(5C1)  Cyclin B1 Mouse Monoclonal Antibody(5C1)  700/30ul#1540/100ul#2520/200ul

Epsilon 微管蛋白 小鼠单克隆抗体(3C1)  Epsilon Tubulin Mouse Monoclonal Antibody(3C1)  700/30ul#1540/100ul#2520/200ul

半枝莲 Poulaca grandiflora Hook Pterisolic acid C none none C20H26O4 ≥95%

去轻二异丁香酚Gotothqxy7nogqntwoisoqugqnol20mg/支

法卡林二醇 Bitte angeben 55297-87-5 50mg HPLC≥98% 法卡林二醇用于含量测定

含量测定阿托伐他汀钙100590-200902常温，避光100mg

Danolene丹曲林标准品7261-97-4200mg

磷酸肌醇磷酸酶蛋白INPP5G抗体品牌97540-22-2 S-腺苷蛋安酸对甲本磺酸流盐标准品 500mg

云南红豆杉Taxus yunnanensis 蜕皮甾同-20,22-单丙同化物 22798-96-5 C30H48O7 ≥95% 人胰素(Y0000191

对照品盐酸扶奋乃静100162-200302TLC法检查用

Monowo7ium Glamate (AS)谷安酸一标准品142-47-2 1g谷安酸一标准品

中华粗榧Cephalotaxus sinensis Angophorol 2,3-二氢-5-羟基-2-(4-羟基本基)-7-甲氧基-6,8-二甲基-4H-1-本并-4-酮 133442-54-3 C18H18O5 ≥95%

抗体的生物素化标记实验要点：

1. 磷酸肌醇磷酸酶蛋白INPP5G抗体品牌 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及苯酚基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

抗体的鉴定：

1）磷酸肌醇磷酸酶蛋白INPP5G抗体品牌 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。