产品订购信息：
英文名称  Anti-SNAPIN/SNAP25BP 

中文名称   突触相关蛋白25结合蛋白抗体费用 

别    名  SNAP associated protein; SNAP-25-binding protein; SNAP-associated protein; SNAP25BP; SNAPAP; SNAPIN; SNAPN_HUMAN; SNARE associated protein snapin; SNARE-associated protein Snapin; Synaptosomal associated protein 25 binding protein; Synaptosomal-associated protein 25-binding protein; OTTHUMP00000035157.

浓    度   1mg/1ml

规 格  0.1ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit 

产品类型   一抗  

研究领域    细胞生物 神经生物学 信号转导 细胞类型标志物

蛋白分子量  predicted molecular weight: 15kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human SNAP25BP 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

突触相关蛋白25结合蛋白抗体费用 产品应用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 The protein encoded by this gene is a coiled-coil-forming protein that associates with the SNARE (soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptor) complex of proteins and the BLOC-1 (biogenesis of lysosome-related organelles) complex. Biochemical studies have identified additional binding partners. As part of the SNARE complex, it is required for vesicle docking and fusion and regulates neurotransmitter release. The BLOC-1 complex is required for the biogenesis of specialized organelles such as melanosomes and platelet dense granules. Mutations in gene products that form the BLOC-1 complex have been identified in mouse strains that are models of Hermansky-Pudlak syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2012].

Function : May modulate a step between vesicle priming, fusion and calcium-dependent neurotransmitter release by potentiating the interaction of synaptotagmins with the SNAREs and the plasma-membrane-associated protein SNAP25. Its phosphorylation state influences exocytotic protein interactions and may regulate synaptic vesicle exocytosis. May also have a role in the mechanisms of SNARE-mediated membrane fusion in non-neuronal cells.

Subunit : Interacts with CSNK1D, SNAP23 and STX4A but not with STX1A, VAMP2 and SYT1. Interacts with SNAP25; the interaction with SNAP25 is increased by its phosphorylation (By similarity). Component of the biogenesis of lysosome-related organelles complex 1 (BLOC-1) composed of BLOC1S1, BLOC1S2, BLOC1S3, BLOC1S4, BLOC1S5, BLOC1S6, DTNBP1/BLOC1S7 and SNAPIN/BLOC1S8. Octamer composed of one copy each BLOC1S1, BLOC1S2, BLOC1S3, BLOC1S4, BLOC1S5, BLOC1S6, DTNBP1/BLOC1S7 and SNAPIN/BLOC1S8. The BLOC-1 complex associates with the AP-3 protein complex and membrane protein cargos. Associates with the SNARE complex. Interacts with CEP110, NANOS1, PUM2 and RGS7. Interacts with human cytomegalovirus/HHV-5 protein UL70.

Subcellular Location : Cytoplasm (By similarity). Membrane; Peripheral membrane protein; Cytoplasmic side (By similarity). Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side (By similarity). Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Peripheral membrane protein; Cytoplasmic side (Potential). Cell junction, synapse, synaptosome. Cytoplasm, perinuclear region. Golgi apparatus membrane (By similarity). Note=May be cytoplasmic and peripheral membrane bound or anchored to the vesicular membrane through an N-terminal signal anchor (By similarity). Co-localizes with NANOS1 and PUM2 in the perinuclear region of germ cells.

Tissue Specificity : Expressed in male germ cells of adult testis (at protein level).

Post-translational modifications : Phosphorylated by CSNK1D/CK1 (By similarity).

Similarity : Belongs to the SNAPIN family.

Database links : UniProtKB/Swiss-Prot: O95295.1

痕量胺关联受体6 多克隆抗体  TAAR6 Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

痕量胺相关受体8 多克隆抗体  TAAR8 Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

痕迹胺关联受体9 多克隆抗体  TAAR9 Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

TAK1结合蛋白3 多克隆抗体  TAB3 Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

TARDNA结合蛋白 多克隆抗体  TADBP Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

淀粉样蛋白β前体样蛋白1抗体     /抗原74抗体

α2巨球蛋白(α-2M)抗体     /抗原65

ACCSL蛋白抗体     /抗原44抗体

转录激活蛋白crsp70抗体     /抗原3抗体

粘附分子IgG样结构域蛋白1抗体     /抗原2抗体

原钙粘附蛋白α9抗体     PUM2蛋白抗体

原钙粘附蛋白α7抗体     PUM1蛋白抗体

原钙粘附蛋白α8抗体     PSP抗体

钙粘蛋白相关神经受体8抗体     PSF2蛋白抗体

原钙粘蛋白15抗体     PSD95相关紧密连接蛋白PATJ抗体

土荆皮Coex Pseudolaricis Pseudolaric Acid C 土荆皮 82601-41-0 C21H26O7 ≥98%

科罗索醋CorosolicccidHPLC≥98%;20mg/支

远志皂苷元 Senegenin 2469-34-3 20mg/支 HPLC≥98% 用于含量测定

含量测定尼群地平100585- 201003 常温，避光100mg

Monoglycerides单甘酯标准品68990-53-4 125mg

突触相关蛋白25结合蛋白抗体费用5538-41-0 录儿价分相关物质A标准品 25mg

云南萝芙木Rauvolfia yunnanensis 北升宁 142542-89-0 C14H20O8 ≥98.5% 异环灵酰安 杂质B(I0060004

萎锈灵标准品 环丙酰安标准品 联本进酯标准品

Calcium Ascorbate抗坏血酸钙标准品5743-28-2200mg抗坏血酸钙标准品

阿魏酸乙酯 4046-02-0 HPLC≥98%;20mg 订购|咨询

抗体的生物素化标记实验要点：

1. 突触相关蛋白25结合蛋白抗体费用 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及苯酚基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

抗体的鉴定：

1）突触相关蛋白25结合蛋白抗体费用 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。