抗体的生物素化标记实验要点：

1. 分泌模块化钙结合蛋白2抗体（平滑肌相关蛋白2）费用 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及苯酚基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

产品订购信息：
英文名称  Anti-SMOC2 

中文名称   分泌模块化钙结合蛋白2抗体（平滑肌相关蛋白2）费用 

别    名  Secreted modular calcium binding protein 2; SMAP 2; SMAP2; SMOC 2; Smooth muscle associated protein 2; SPARC related modular calcium binding 2; dJ421D16.1; MST117; MSTP117; bA270C4A.1; bA37D8.1; dJ421D16.1 (novel thyroglobulin type 1 repeat containing protein); MSTP140; SMOC2_HUMAN.

浓    度   1mg/1ml

规 格  0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, Sheep 

产品类型   一抗  

研究领域    心 细胞生物 信号转导 生长因子和激素 转录调节因子 内皮细胞

蛋白分子量  predicted molecular weight: 47kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human SMOC2 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

分泌模块化钙结合蛋白2抗体（平滑肌相关蛋白2）费用 产品应用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 SMOC 2 (Secreted modular calcium binding protein 2) is a secreted modular protein containing an EF hand calcium binding domain homologous to that in BM40. It consists of two thyroglobulin like domains, a follistatin like domain and a novel domain found only in the homologous SMOC 1. SMOC 1 and 2 form a separate group within the BM40 family. SMOC 2 is a glycoprotein with a calcium dependent conformation. SMOC 2 contains 2 EF hand calcium binding domains, 1 Kazal like domain and 2 thyroglobulin type I domains.

Function : Promotes matrix assembly and cell adhesiveness (By similarity). Can stimulate endothelial cell proliferation, migration, as well as angiogenesis.

Subunit : Binds various proteins from the extracellular matrix (By similarity).

Subcellular Location : Secreted, extracellular space, extracellular matrix, basement membrane (By similarity).

DISEASE : Defects in SMOC2 are the cause of dentin dysplasia typ1 (DTDP1) [MIM:125400]. A dental defect in which both primary and secondary dentitions are affected. The clinical crowns of both permanent and deciduous teeth are of normal shape, form and color in most cases, although they may be slightly opalescent and blue or brown. Teeth may be very mobile and exfoliate spontaneously because of inadequate root formation. On radiographs, the roots are short and may be more pointed than normal. Pulp chambers are usually absent except for a chevron-shaped remnant in the crown. Root canals are usually absent.

Similarity : Contains 2 EF-hand domains.

Contains 1 Kazal-like domain.

Contains 2 thyroglobulin type-1 domains.

Database links : UniProtKB/Swiss-Prot: Q9H3U7.2

抗体的鉴定：

1）分泌模块化钙结合蛋白2抗体（平滑肌相关蛋白2）费用 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。

Sjogren氏综合征/自身抗原1 多克隆抗体  SSA27 Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

peter-pan同源物 多克隆抗体  SSF1 Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

促生长素抑制蛋白受体3 多克隆抗体  SSR3 Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

促生长素抑制蛋白受体5 多克隆抗体  SSR5 Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

信号序列受体γ亚基 多克隆抗体  SSRG Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

抗激素受体1B抗体     转移抑制基因GDIA2抗体

二磷酸腺苷核糖基化因子鸟嘌呤核苷酸交换因子2抗体     转移抑制基因GDIA1抗体

α增强子结合蛋白1抗体     转移抑制蛋白BAMBI抗体

脊髓小脑10抗体     转移抑制蛋白1抗体

Allgrove综合征相关蛋白抗体     转移抑制蛋白1

中断蛋白1/癌蛋白18(phospho Ser38) 多克隆抗体  Op18 (phospho Ser38) Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

p21 (phospho Thr145) 多克隆抗体  p21 (phospho Thr145) Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

p27 (phospho Ser10) 多克隆抗体  p27 (phospho Ser10) Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

p38 (phospho Thr180) 多克隆抗体  p38 (phospho Thr180) Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

p38 (phospho Tyr182) 多克隆抗体  p38 (phospho Tyr182) Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

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