抗体的生物素化标记实验要点：

1. 泛素结合蛋白P62抗体规格 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及苯酚基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

产品订购信息：
英文名称  Anti-SQSTM1/p62 

中文名称   泛素结合蛋白P62抗体规格 

别    名  A170; EBI 3 associated protein of 60 kDa; EBI3 associated protein of 60 kDa; EBI3-associated protein of 60 kDa; EBIAP; MGC127197; ORCA; OSF-6; Osi; OSIL; Oxidative stress induced like; p60; p62; p62B; Paget disease of bone 3; PDB 3; PDB3; Phosphotyrosine independent ligand for the Lck SH2 domain of 62 kDa; Phosphotyrosine independent ligand for the Lck SH2 domain p62; Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa; PKC-zeta-interacting protein; Protein kinase C-zeta-interacting protein; Sequestosome 1; Sequestosome-1; SQSTM 1; SQSTM_HUMAN; Sqstm1; STAP; STONE14; Ubiquitin binding protein p62; Ubiquitin-binding protein p62; ZIP 3; ZIP; ZIP3

浓    度   1mg/1ml

规 格  0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep 

产品类型   一抗  

研究领域    心 染色质和核信号 信号转导 细胞凋亡 转录调节因子 表观遗传学

蛋白分子量  predicted molecular weight: 48kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human SQSTM1/p62 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

泛素结合蛋白P62抗体规格 产品应用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 Adapter protein which binds ubiquitin and may regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. May play a role in titin/TTN downstream signaling in muscle cells. May regulate signaling cascades through ubiquitination. Adapter that mediates the interaction between TRAF6 and CYLD (By similarity). May be involved in cell differentiation, apoptosis, immune response and regulation of K(+) channels.

Function : Adapter protein which binds ubiquitin and may regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. May play a role in titin/TTN downstream signaling in muscle cells. May regulate signaling cascades through ubiquitination. Adapter that mediates the interaction between TRAF6 and CYLD (By similarity). May be involved in cell differentiation, apoptosis, immune response and regulation of K(+) channels.

Subunit : Cytoplasm. Late endosome. Nucleus. Endoplasmic reticulum. Note=Sarcomere. In cardiac muscles localizes to the sarcomeric band. Localizes to late endosomes. May also localize to the nucleus. Accumulates in neurofibrillary tangles and in Lewy bodies of neurons from individuals with Alzheimer and Parkinson disease respectively. Enriched in Rosenthal fibers of pilocytic astrocytoma. In liver cells, accumulates in Mallory bodies associated with alcoholic hepatitis, Wilson disease, indian childhood cirrhosis and in hyaline bodies associated with hepatocellular carcinoma. Colocalizes with TRIM13 in the perinuclear endoplasmic reticulum.

Subcellular Location : Cytoplasm. Late endosome. Nucleus. Sarcomere (By similarity). In cardiac muscles localizes to the sarcomeric band (By similarity). Localizes to late endosomes. May also localize to the nucleus. Accumulates in neurofibrillary tangles and in Lewy bodies of neurons from individuals with Alzheimer and Parkinson disease respectively. Enriched in Rosenthal fibers of pilocytic astrocytoma. In liver cells, accumulates in Mallory bodies associated with alcoholic hepatitis, Wilson disease, indian childhood cirrhosis and in hyaline bodies associated with hepatocellular carcinoma.

Tissue Specificity : Ubiquitously expressed.

Post-translational modifications : Phosphorylated. May be phosphorylated by PRKCZ (By similarity). Phosphorylated in vitro by TTN.

DISEASE : Defects in SQSTM1 are a cause of Paget disease of bone (PDB) [MIM:602080]. PDB is a metabolic bone disease affecting the axial skeleton and characterized by focal areas of increased and disorganized bone turn-over due to activated osteoclasts. Manifestations of the disease include bone pain, deformity, pathological fractures, deafness, neurological complications and increased risk of osteosarcoma. PDB is a chronic disease affecting 2 to 3% of the population above the age of 40 years.

Similarity : Contains 1 OPR domain.

Contains 1 UBA domain.

Contains 1 ZZ-type zinc finger.

抗体的鉴定：

1）泛素结合蛋白P62抗体规格 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。

杆状缺失区基因1 多克隆抗体  RTDR1 Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

网状蛋白2 多克隆抗体  RTN2 Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

网状蛋白4受体 多克隆抗体  RTN4R Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

松弛素/样肽受体1 多克隆抗体  RXFP1 Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

类视黄醇X受体α 多克隆抗体  RXRA Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

载脂蛋白A1抗体     子宫内膜抗体

髓系、淋巴、混合系易位基因10抗体     滋养层细胞抗原3β-HSD抗体

腺瘤样抗体     滋养层细胞抗原3β7抗体

腺苷酸环化酶1抗体     椎骨蛋白2抗体

雄激素受体相关蛋白24抗体     装配衔接蛋白180抗体

组蛋白H2A.X (phospho Ser139) 多克隆抗体  Histone H2A.X (phospho Ser139) Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

组蛋白H3 (phospho Ser10) 多克隆抗体  Histone H3 (phospho Ser10) Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

组蛋白H3 (phospho Ser28) 多克隆抗体  Histone H3 (phospho Ser28) Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

Hrs (phospho Tyr216) 多克隆抗体  Hrs (phospho Tyr216) Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

热休克蛋白27 (phospho Ser15) 多克隆抗体  HSP27 (phospho Ser15) Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

何首乌Polygonum multiflorum Polygonal (3R,4aS,8aS)-3-羟基-5,5,8a-基-3,4,4a,6,7,8-六氢-2-甲醛 72537-20-3 C14H22O2 ≥98%

莪术二同ZqdocrytwokqtonqHPLC≥98%,20mg/支

重组人干扰素-α1b Recombinant Human Interferon-alpha 1b (rhIFN-α1b) 分子量为19.4kD 纯度>95% RP-HPLC与还原/非还原SDS-PAGE银染胶分析方法

中药

泛素结合蛋白P62抗体规格酪蛋白 Casein 7g/瓶

獐牙菜苦甙Swqicmcrin17388-39-2HPLC≥98%;20mg/vial

氧化苦参减;苦参素 Oxymaine 16837-52-8 20mg HPLC≥98% 用于含量测定

标准品毒毛旋花子苷G870405效价测定

古伦宾 Columbin 546-97-4 20mg HPLC≥98% 用于含量测定