产品订购信息：
英文名称  Anti-SLC5A8 

中文名称   钠碘转运体蛋白8抗体品牌 

别    名  AIT; Apical iodide transporter; Electrogenic sodium monocarboxylate cotransporter; SC5A8_HUMAN; SLC5A8; SMCT; SMCT1; sodium coupled monocarboxylate transporter 1; Sodium iodide related cotransporter; Sodium iodide-related cotransporter; Sodium-coupled monocarboxylate transporter 1; solute carrier family 5 iodide transporter member 8; Solute carrier family 5 member 8.

浓    度   1mg/1ml

规 格  0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Chicken, Dog, Cow 

产品类型   一抗  

研究领域     细胞生物 神经生物学 信号转导 细胞凋亡

蛋白分子量  predicted molecular weight: 67kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human SLC5A8 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

钠碘转运体蛋白8抗体品牌 产品应用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 Acts as an electrogenic sodium (Na(+)) and chloride (Cl-)-dependent sodium-coupled solute transporter, including transport of monocarboxylates (short-chain fatty acids including L-lactate, D-lactate, pyruvate, acetate, propionate, valerate and butyrate), lactate, mocarboxylate drugs (nicotinate, benzoate, salicylate and 5-aminosalicylate) and ketone bodies (beta-D-hydroxybutyrate, acetoacetate and alpha-ketoisocaproate), with a Na(+):substrate stoichiometry of between 4:1 and 2:1. Catalyzes passive carrier mediated diffusion of iodide. Mediates iodide transport from the thyrocyte into the colloid lumen through the apical membrane. May be responsible for the absorption of D-lactate and monocarboxylate drugs from the intestinal tract. Acts as a tumor suppressor, suppressing colony formation in colon cancer, prostate cancer and glioma cell lines. May play a critical role in the entry of L-lactate and ketone bodies into neurons by a process driven by an electrochemical Na(+) gradient and hence contribute to the maintenance of the energy status and function of neurons.

Function : Acts as an electrogenic sodium (Na(+)) and chloride (Cl-)-dependent sodium-coupled solute transporter, including transport of monocarboxylates (short-chain fatty acids including L-lactate, D-lactate, pyruvate, acetate, propionate, valerate and butyrate), lactate, mocarboxylate drugs (nicotinate, benzoate, salicylate and 5-aminosalicylate) and ketone bodies (beta-D-hydroxybutyrate, acetoacetate and alpha-ketoisocaproate), with a Na(+):substrate stoichiometry of between 4:1 and 2:1. Catalyzes passive carrier mediated diffusion of iodide. Mediates iodide transport from the thyrocyte into the colloid lumen through the apical membrane. May be responsible for the absorption of D-lactate and monocarboxylate drugs from the intestinal tract. Acts as a tumor suppressor, suppressing colony formation in colon cancer, prostate cancer and glioma cell lines. May play a critical role in the entry of L-lactate and ketone bodies into neurons by a process driven by an electrochemical Na(+) gradient and hence contribute to the maintenance of the energy status and function of neurons.

Subcellular Location : Apical cell membrane; Multi-pass membrane protein.

Tissue Specificity : Expressed in normal thyroid, localized at the apical pole of thyroid cells facing the colloid lumen, but expression profoundly decreased in thyroid carcinomas. Expressed in normal colon but absent in colon aberrant crypt foci and colon cancers. Present in normal kidney cortex, brain, prostate, gastric mucosa and breast tissue but was significantly down-regulated in primary gliomas, gastric cancer, prostate tumors and breast tumors.

Similarity : Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.

Database links : UniProtKB/Swiss-Prot: Q8N695.2

环指蛋白41 多克隆抗体  RNF41 Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

环指蛋白5 多克隆抗体  RNF5 Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

环指蛋白6 多克隆抗体  RNF6 Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

环指蛋白81 多克隆抗体  RO52 Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

异质性胞核核糖核蛋白A0 多克隆抗体  ROA0 Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

真核翻译起始因子2C4抗体     组蛋白H3-K9甲基转移酶抗体

Rho GTP酶激活蛋白15抗体     组蛋白H3K9甲基转移酶4抗体

基因结合蛋白ARID1B抗体     组蛋白H3 K9甲基转移酶抗体

E3连接酶蛋白ARIH2抗体     组蛋白H2B抗体

ADP核糖基化样因子1抗体     组蛋白H2B.s抗体

组蛋白H3 (Di Methyl Lys79) 单克隆抗体(Q7)  Histone H3 (Di Methyl Lys79) Monoclonal Antibody(Q7)  700/30ul#1540/100ul#2520/200ul

组蛋白H3 (Tri Methyl Lys36) 单克隆抗体(Q12)  Histone H3 (Tri Methyl Lys36) Monoclonal Antibody(Q12)  700/30ul#1540/100ul#2520/200ul

Oct1 单克隆抗体  Oct1 Monoclonal Antibody  700/30ul#1540/100ul#2520/200ul

CD16 单克隆抗体  CD16 Monoclonal Antibody  700/30ul#1540/100ul#2520/200ul

组蛋白H3 (Tri Methyl Lys79) 单克隆抗体(3G3)  Histone H3 (Tri Methyl Lys79) Monoclonal Antibody(3G3)  700/30ul#1540/100ul#2520/200ul

苍耳Xanthium sibiricum p-Menth-8-ene-1,2-diol 1-羟基异二氢葛缕醇 57457-97-3 C10H18O2 ≥95%

大车前苷ThqccglycosidqHPLC≥98%，20mg/支

安苄青每素 安苄西林三水酸、安苄青每素三水酸、三水安苄西林、三水合安苄青每素、水合安苄青每素 Ampicillin 7177-48-2 ≥98.5% HPLC

含量测定愈创甘油醚100528-200401常温，避光50mg

Iodixanol 典克沙醇标准品92339-11-2 2

钠碘转运体蛋白8抗体品牌异土木香内酯 Isoalantolactone (≥98%,HPLC) 23720-80-1 20MG 标准品

掌叶防己 Jaorrhiza palmate( DC.)Miers 大黄藤素 3486-67-7 C21H22NO4+ ≥98% 亮丙瑞林 - 对照谱图(L0 76001

Curcumin姜黄素458-37-720mg/支

人皮肤参考显微照片标准品ea

香叶子树Lindera sychnifolia Bi-linderone none 1227375-09-8 C34H32O10 ≥95%

抗体的生物素化标记实验要点：

1. 钠碘转运体蛋白8抗体品牌 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及苯酚基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

抗体的鉴定：

1）钠碘转运体蛋白8抗体品牌 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。