产品订购信息：
英文名称  Anti-SAP62 

中文名称   剪接体相关蛋白62抗体品牌 

别    名  PRPF11; SF3a66; SAP 62; SAP-62; Spliceosome-associated protein 62; splicing factor 3a subunit 2; SF3A2_HUMAN.

浓    度   1mg/1ml

规 格  0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Chicken, Dog, Cow 

产品类型   一抗  

研究领域    细胞生物 表观遗传学

蛋白分子量  predicted molecular weight: 49kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human SAP62 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

剪接体相关蛋白62抗体品牌 产品应用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 SAP 62, also known as SF3A2 (Splicing factor 3A subunit 2), PRP11, PRPF11 or SF3a66, is a 464 amino acid protein that contains one matrin-type zinc finger and belongs to the SF3A2 family. Localized to the nucleus, SAP 62 is a subunit of the SF3A splicing factor, a heterotrimeric complex comprised of three subunits that act in tandem to mediate the binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. The SF3A complex is necessary for the conversion of 15S U2 snRNP into the active 17S protein that performs directly in pre-mRNA splicing events. Functioning as the second subunit of the complex, SAP 62 interacts with subunit 1 (SAP 114) via its N-terminus while simultaneously binding to 15S U2 snRNP via its zinc finger domain. In addition to its role in RNA splicing, SAP 62 is thought to act independently as a microtubule-binding protein.

Function : Subunit of the splicing factor SF3A required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex.

Subunit : Component of splicing factor SF3A which is composed of three subunits; SF3A3/SAP61, SF3A2/SAP62, SF3A1/SAP114. SF3A associates with the splicing factor SF3B and a 12S RNA unit to form the U2 small nuclear ribonucleoproteins complex (U2 snRNP). Identified in the spliceosome C complex. Interacts with HTATSF1.

Subcellular Location : Nuclear

Tissue Specificity : Component of splicing factor SF3A which is composed of three subunits; SF3A3/SAP61, SF3A2/SAP62, SF3A1/SAP114. SF3A associates with the splicing factor SF3B and a 12S RNA unit to form the U2 small nuclear ribonucleoproteins complex (U2 snRNP). Identified in the spliceosome C complex. Interacts with HTATSF1.

Similarity : Belongs to the SF3A2 family.

Contains 1 matrin-type zinc finger.

Database links : UniProtKB/Swiss-Prot: Q15428.2

RIO激酶2 多克隆抗体  RIOK2 Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

受体(TNFRSF)结合丝氨酸/苏氨酸激酶1 多克隆抗体  RIPK1 Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

受体(TNFRSF)结合丝氨酸/苏氨酸激酶2(磷酸化-Ser176) 多克隆抗体  RIPK2 (Phospho-Ser176) Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

受体(TNFRSF)结合丝氨酸/苏氨酸激酶3 多克隆抗体  RIPK3 Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

GTP结合蛋白RIT2 多克隆抗体  RIT2 Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

衰老相关蛋白5抗体     组蛋白乙酰转移酶PCAF抗体

釉基质蛋白抗体     组蛋白乙酰转移酶MOF抗体

磷酸化腺苷单磷酸活化蛋白激酶α2抗体     组蛋白相关Bmi1抗体

磷酸化雄激素受体抗体     组蛋白替换精蛋白DGGR1抗体

锚定蛋白重复结构域蛋白激酶ANKK1抗体     组蛋白去乙酰化酶9抗体

组蛋白去乙酰化酶3 多克隆抗体  HDAC3 Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

组蛋白去乙酰化酶4 多克隆抗体  HDAC4 Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

组蛋白去乙酰化酶5 多克隆抗体  HDAC5 Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

组蛋白去乙酰化酶5/9 多克隆抗体  HDAC5/9 Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

组蛋白去乙酰化酶6 多克隆抗体  HDAC6 Polyclonal Antibody  700/30ul#1540/100ul#2520/200ul

车前草Plantagodepressa Willd Plantamajoside 大车前苷 104777-68-6 C29H36O16 ≥98%

去异波尔定Norisoboldinq10mg/支

7-安基-4-甲基 7-aMino-4-metxylcouMarin 26093-31-2 HPLC≥98% HPLC

含量测定异丙托溴胺100522-200601常温，避光100mg

Ioxaglic Acid典克沙酸标准品59017-64-0 100mg

剪接体相关蛋白62抗体品牌NA 录喹相关化合物E标准品(NA) 15mg

柘Cudrania icuspidata 香橙素，香树素 480-20-6 C15H12O6 ≥98.5% 水杨酸甲酯190 7-100

8-Epicrepiside E 93395-30-3

Chloroxyleol录儿价分标准品1988-4-6125mg录儿价分标准品

羊藿素 HPLC≥98%;20mg 订购|咨询

抗体的生物素化标记实验要点：

1. 剪接体相关蛋白62抗体品牌 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及苯酚基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

抗体的鉴定：

1）剪接体相关蛋白62抗体品牌 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。