产品订购信息：
英文名称  Anti-SLC37A4/G6PT2 

中文名称   葡萄糖-6磷酸转运蛋白抗体规格 

别    名  G6PT2; GSD1b; GSD1c; GSD1d; TRG19; G6PT1; G6PT3; Glucose-5-phosphate transporter; Glucose-6-phosphatase, transport (glucose) protein 3 antibody; Glucose-6-phosphatase, transport (glucose-6-phosphate) protein 1; Glucose-6-phosphatase, transport (phosphate/pyrophosphate) protein 2; Glucose-6-phosphate translocase; Glucose-6-phosphate transporter 1; Microsomal glucose-6-phosphate transporter; Solute carrier family 37 (glucose-6-phosphate transporter), member 4; MGC15729; PRO0685; G6PT1_HUMAN.

浓    度   1mg/1ml

规 格  0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Dog, Pig, Cow 

产品类型   一抗  

研究领域     细胞生物 免疫学 转录调节因子 转运蛋白

蛋白分子量  predicted molecular weight: 46kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human G6PT2 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

葡萄糖-6磷酸转运蛋白抗体规格 产品应用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 SLC37A4 transports glucose-6-phosphate from the cytoplasm to the lumen of the endoplasmic reticulum. It forms a complex with glucose-6-phosphatase which is responsible for glucose production through glycogenolysis and gluconeogenesis. Hence, it plays a central role in homeostatic regulation of blood glucose levels.

Function : Transports glucose-6-phosphate from the cytoplasm to the lumen of the endoplasmic reticulum. Forms with glucose-6-phosphatase the complex responsible for glucose production through glycogenolysis and gluconeogenesis. Hence, it plays a central role in homeostatic regulation of blood glucose levels.

Subcellular Location : Endoplasmic reticulum membrane; Multi-pass membrane protein

Tissue Specificity : Mostly expressed in liver and kidney

DISEASE : Defects in SLC37A4 are the cause of glycogen storage disease type 1B (GSD1B) [MIM:232220]. GSD1B is a metabolic disorder characterized by impairment of terminal steps of glycogenolysis and gluconeogenesis. GSD1 patients manifest a wide range of clinical symptoms and biochemical abnormalities, including hypoglycemia, severe hepatomegaly due to excessive accumulation of glycogen, kidney enlargement, growth retardation, lactic acidemia, hyperlipidemia, and hyperuricemia. GSD1B patients also present a tendency towards infections associated with neutropenia, relapsing aphthous gingivostomatitis, and inflammatory bowel disease.

Defects in SLC37A4 are the cause of glycogen storage disease type 1C (GSD1C) [MIM:232240].

Defects in SLC37A4 are the cause of glycogen storage disease type 1D (GSD1D) [MIM:232240].

Similarity : Belongs to the major facilitator superfamily. Organophosphate:Pi antiporter (OPA) (TC 2.A.1.4) family.

Anti-Phospho-HSL (Ser552)/FITC 荧光素标记磷酸化荷尔蒙敏感脂肪酶抗体IgGMulti-class antibodies规格： 0.2ml

Anti-PI3K/P85 alpha/FITC 荧光素标记磷脂酰肌醇激酶抗体IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh ASP/AKAP associated Sperm Protein 相关蛋白AKAP抗体 规格 0.2ml

Donkey Anti-Guinea pig IgG/PE-CY5 PE-CY5标记的驴抗豚鼠IgG 0.1ml

Glucose Oxidase 英文名称： 葡萄糖氧化酶抗体 0.2ml

Rhesus antibody Rh Rabbit Anti-mouse IgG/PE-Cy3 PE-Cy3标记的兔抗小鼠IgG 规格 0.1ml

Anti-PI3K/P85 alpha/FITC 荧光素标记磷脂酰肌醇激酶抗体IgGMulti-class antibodies规格： 0.2ml

Anti-DC-SIGN/CD209/CLEC4L/FITC 荧光素标记CD209抗体IgGMulti-class antibodies规格： 0.2ml

Anti-Phospho-PKM2 (Tyr105) /FITC 荧光素标记磷酸化酸激酶M2抗体IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh CaMK2 beta/CaMK2 gamma 钙/钙调素依赖蛋白激酶2b/2γ抗体 规格 0.1ml

Cyclin D3(C-term) 周期素D3抗原 0.5mg

Hoxb3 英文名称： 同源盒蛋白HoxB3抗体 0.2ml

Rhesus antibody Rh SEZ6L 样相关蛋白6抗体 规格 0.2ml

Anti-Phospho-PKM2 (Tyr105) /FITC 荧光素标记磷酸化酸激酶M2抗体IgGMulti-class antibodies规格： 0.2ml

NA(Human neuraminidase) ELISA Kit 人神经氨酸酶Multi-class antibodies规格： 48T

Anti-NEDD4L/NEDD4-2 泛素连接酶NEDD4-2抗体Multi-class antibodies规格： 0.2ml

Rhesus antibody Rh IL-6 白介素6 规格 0.1ml

MMP-3 ELISA kit 大鼠基质金属蛋白酶3 96T

phospho-PRKCZ(Thr500) 英文名称： 磷酸化蛋白激酶C(T500)抗体 0.2ml

C8orf55 英文名称： 8号染色体开放阅读框55抗体 0.2ml

Anti-NEDD4L/NEDD4-2 泛素连接酶NEDD4-2抗体Multi-class antibodies规格： 0.2ml

HAVCR2 Others Mouse 小鼠 TIM3 / HAVCR2 人细胞裂解液 (阳性对照)

小鼠纹状体神经元MN-s

人胚;MRC-5

DMS153细胞，人小细胞细胞 大鼠肾上腺嗜铬细胞瘤细胞,PC12细胞 CM-H127人晶状体上皮细胞完全培养基100mL

PANC-1(人*癌细胞) 5×106cells/瓶×2

Promocell C-28020 Hematopoietic ProgenitorMedium, 造血祖细胞培养基(即用型) 100ml

CL-0432RTE(大鼠气管上皮细胞)5×106cells/瓶×2

IL23 Others Mouse 小鼠 IL-23 / IL23A & IL12B Heterodimer 人细胞裂解液 (阳性对照)

人肠微内皮细胞RNAHIMEC miRNA5 μg

RBE细胞，人细胞 小鼠艾氏腹水瘤细胞,ECA细胞 人羊膜上皮细胞

M14(人细胞) 5×106cells/瓶×2

NHEK.f pooled Pellet 正常人表皮角质细胞团块(少年者，混合来源) > 1 mio.cells 内皮细胞培养基ECM

葡萄糖-6磷酸转运蛋白抗体规格 CL-0430RS1(大鼠皮肤成纤维样细胞)5×106cells/瓶×2

IL22RA1 Others Canine 狗 IL22RA1 / IL22R 人细胞裂解液 (阳性对照)

人食道上皮细胞RNAHEEpiC miRNA5 μg

兔间变表皮鳞株;VX2 细胞，22RV1细胞 MDCK superdome细胞，狗肾细胞隆突型

人永生化淋巴细胞;CNLMG-B5537LC

HA Others H3N2 甲型 H3N2 (A/Aichi/2/1968) 血凝素HA1 (Hemagglutinin) 人细胞裂解液 (阳性对照)

抗体的生物素化标记实验要点：

1. 葡萄糖-6磷酸转运蛋白抗体规格 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及苯酚基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

抗体的鉴定：

1）葡萄糖-6磷酸转运蛋白抗体规格 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。