产品订购信息：
英文名称  Anti-SENP3 

中文名称   类泛素蛋白3抗体规格 

别    名  Sentrin/SUMO specific protease 3; Sentrin specific protease 3; SMT3IP1; SSP3; SUMO1 specific protease 3; SUSP3; SENP3_HUMAN.

浓    度   1mg/1ml

规 格  0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Dog, Pig, Horse, Rabbit 

产品类型   一抗  

研究领域     细胞生物 免疫学 转录调节因子

蛋白分子量  predicted molecular weight: 65kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human SENP3 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

类泛素蛋白3抗体规格 产品应用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 The SENP3 protease releases SUMO2 and SUMO3 monomers from sumoylated substrates, but has only weak activity against SUMO1 conjugates. It deconjugates SUMO2 from MEF2D, which increases its transcriptional activation capability.

Function : Protease that releases SUMO2 and SUMO3 monomers from sumoylated substrates, but has only weak activity against SUMO1 conjugates. Deconjugates SUMO2 from MEF2D, which increases its transcriptional activation capability. Deconjugates SUMO2 and SUMO3 from CDCA8. Redox sensor that, when redistributed into nucleoplasm, can act as an effector to enhance HIF1A transcriptional activity by desumoylating EP300. Required for rRNA processing through deconjugation of SUMO2 and SUMO3 from nucleophosmin, NPM1. Plays a role in the regulation of sumoylation status of ZNF148. Functions as a component of the Five Friends of Methylated CHTOP (5FMC) complex; the 5FMC complex is recruited to ZNF148 by methylated CHTOP, leading to desumoylation of ZNF148 and subsequent transactivation of ZNF148 target genes.

Subunit : Binds to SUMO1 and SUMO3 (By similarity). Component of some MLL1/MLL complex, at least composed of the core components MLL, ASH2L, HCFC1/HCF1, WDR5 and RBBP5, as well as the facultative components BAP18, CHD8, E2F6, HSP70, INO80C, KANSL1, LAS1L, MAX, MCRS1, MGA, MYST1/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Interacts with EP300, NPM1 and CDCA8. Component of the 5FMC complex, at least composed of PELP1, LAS1L, TEX10, WDR18 and SENP3; the complex interacts with methylated CHTOP and ZNF148. Interacts with NOL9.

Subcellular Location : Cytoplasm (By similarity). Nucleus, nucleolus. Nucleus, nucleoplasm. Note=Mainly found in the nucleoplasm, with low levels detected in the cytoplasmic and chromatin fractions (By similarity). Redistributes between the nucleolus and the nucleoplasm in response to mild oxidative stress.

Similarity : Belongs to the peptidase C48 family.

Database links : UniProtKB/Swiss-Prot: Q9H4L4.2

CH ELISA Kit 大鼠胆固醇Multi-class antibodies规格： 48T

Anti-Phospho-TAK1 (Ser412) 磷酸化转化生长因子β活化激酶1Multi-class antibodies规格： 0.1ml

Rhesus antibody Rh Goat Anti-Chicken IgG/PE-Cy7 PE-Cy7标记的羊抗鸡IgG 规格 0.1ml

5-HT(Mouse 5-Hydroxytryptamine) ELISA Kit 小鼠5羟色胺 96T

NTN3 英文名称： 轴突生长诱导因子3抗体 0.2ml

BMPER 英文名称： BMP内皮细胞调节蛋白抗体 0.2ml

Anti-Phospho-TAK1 (Ser412) 磷酸化转化生长因子β活化激酶1Multi-class antibodies规格： 0.1ml

Anti-EAAT1/Glast /FITC 荧光素标记胶质细胞谷氨酸运载蛋白1 抗体IgGMulti-class antibodies规格： 0.2ml

Anti-MITF/FITC 荧光素标记小眼相关转录因子抗体IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh C4orf27 4号染色体开放阅读框27抗体 规格 0.2ml

CD36 CD36(多肽) 0.5mg

HSP27 英文名称： 热休克蛋白27抗体 0.1ml

Rhesus antibody Rh SCF 干细胞生长因子抗体 规格 0.1ml

Anti-MITF/FITC 荧光素标记小眼相关转录因子抗体IgGMulti-class antibodies规格： 0.2ml

ANG-1(Human Angiopoietin 1) ELISA Kit 人生成素1Multi-class antibodies规格： 48T

Phospho-PAK4 (Ser474)/PAK5 (Ser602)/PAK6 (Ser560) 磷酸化p21激活激酶4、5、6抗体Multi-class antibodies规格： 0.1ml

Rhesus antibody Rh Hpt/Haptoglobin 结合珠蛋白/触珠蛋白抗体 规格 0.1ml

ATGA/TGAB ELISA Kit 大鼠抗甲状腺球蛋白抗体 96T

Phospho-PDGFRA(Tyr849)/PDGFRB(Tyr857) 英文名称： 磷酸化血小板源性生长因子受体α/β抗体 0.1ml

CACNB4 英文名称： L型电压依赖型钙通道β4抗体 0.2ml

Phospho-PAK4 (Ser474)/PAK5 (Ser602)/PAK6 (Ser560) 磷酸化p21激活激酶4、5、6抗体Multi-class antibodies规格： 0.1ml

FLT3 Others Mouse 小鼠 FLT-3 / CD135 / FLK-2 人细胞裂解液 (阳性对照)

细胞冻存液CFM

人胃腺癌细胞;BGC-823

CCC-HEL-1细胞，人胚肝二倍体细胞 小鼠腹水瘤细胞,SAC-II B2细胞 EB病毒转化的绒猴淋巴细胞;B95-8

MLTC-1(小鼠间质细胞瘤细胞) 5×106cells/瓶×2

Promocell C-23110 Fibroblast Growth Medium KIT,成纤维细胞生长培养基套装 500ml

CL-0291MKN-28(人高转移细胞)5×106cells/瓶×2

LTF Others Mouse 小鼠 Lactoansferrin / LTF 人细胞裂解液 (阳性对照)

小鼠*导管上皮细胞完全培养基 100mL

PC14人细胞 PC14 human lung cancer cells DMEM+10% FBS

IL36RN Protein Mouse 重组小鼠 IL1F5 / IL1RP3 蛋白

P3/NSI/1-Ag4-1 [NS-1] (小鼠细胞) 5×106cells/瓶×2 MC3T3-E1 Subclone 14(小鼠原成骨细胞)

类泛素蛋白3抗体规格 CL-0288MA-891(小鼠癌高转移细胞)5×106cells/瓶×2

CLEC4F Others Rat 大鼠 CLEC4F / CLECSF13 人细胞裂解液 (阳性对照)

人前脂肪细胞-内脏cDNAHPA-v cDNA

BCaP-37细胞，人癌细胞 猴肾细胞系,MA-104细胞 肠内皮细胞Many types of cells包装：5 × 105次方(1ml)

TE-13(人细胞) 5×106cells/瓶×2

HBdMEC Pellet 人膀胱微内皮细胞团块 > 1 mio.cells 胰酶/EDTA消化液T/E

抗体的生物素化标记实验要点：

1. 类泛素蛋白3抗体规格 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及苯酚基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

抗体的鉴定：

1）类泛素蛋白3抗体规格 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。