全、新、优、品、好四大特点：

14-3-3σ抗体说明书 全：公司提供上万种产品，涵盖了生物试剂，elisa试剂盒，标准品，培养基，原装耗材，抗体、培养基、ATCC细胞等，基本上各种科研所需产品在我司都能找到。

新：产品更新速度较快，基本上每周都有新产品出现。

优：产品质量好，投诉比较少。

好：我公司具有优质的技术团队，产品一旦售出，实验过程中遇到困难可提供在线技术咨询。使您使用产品时没有任何的后顾之忧。

14-3-3σ抗体说明书 英文名称  Anti-14-3-3 sigma

中文名称  14-3-3σ抗体说明书 

别    名 14 3 3 protein sigma; 14-3-3 protein sigma; 1433S_HUMAN; Epithelial cell marker protein 1; Er; HME 1; HME1; MGC143283; Mkrn3; Mme1; OTTHUMP00000004242; RP23 137L22.11; SFN; SFN protein; Stratifin; YWHAS.

产品属性：

浓    度  1mg/1ml

规 格  0.2ml/200μg

抗体来源  Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Dog, Pig, Horse, Rabbit, Sheep

产品类型   一抗  

研究领域    细胞生物 染色质和核信号 神经生物学 信号转导 干细胞 转录调节因子

蛋白分子量  predicted molecular weight: 28kDa 

性    状   Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from human 14-3-3 sigma

亚    型  IgG

纯化方法   affinity purified by Protein A

储 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

14-3-3σ抗体说明书 产品应用   WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 14-3-3 proteins regulate many cellular processes relevant to cancer biology, notably apoptosis, mitogenic signaling and cell-cycle checkpoints. Seven isoforms, denoted 14-3-3 b, g, e, z, h, q and s, comprise this family of signaling intermediates. 14-3-3 s, also known as SFN, stratifin, HME1 or YWHAS, is a secreted adaptor protein that is involved in regulating both general and specific signaling pathways. Expressed predominately in stratified squamous keratinising epithelium, 14-3-3 s is able to bind and modify the activity of a large number of proteins, such as KRT17 (Keratin 17), through recognition of a phosphothreonine or phosphoserine motif. When bound to Keratin 17, for example, 14-3-3 s acts to stimulate the Akt/mTOR signaling pathway by upregulating protein synthesis and cell growth. 14-3-3 s also functions to positively mediate IGF-I-induced cell cycle progression and can bind to a variety of translation initiation factors, thus controlling mitotic translation. In response to tumor growth, 14-3-3 s positively regulates the tumor suppressor p53 and increases the rate of p53-regulated inhibition of G2/M cell cycle progression. Multiple isoforms of 14-3-3 s exist due to alternative splicing events.

Function : Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway.

p53-regulated inhibitor of G2/M progression.

Subunit : Homodimer. Interacts with KRT17 and SAMSN1 (By similarity). Found in a complex with XPO7, EIF4A1, ARHGAP1, VPS26A, VPS29, VPS35 and SFN. Interacts with GAB2. Interacts with SRPK2. Interacts with COPS6. Interacts with RFWD2; this interaction leads to proteasomal degradation.

Subcellular Location : Cytoplasm. Nucleus. Secreted. May be secreted by a non-classical secretory pathway.

Tissue Specificity : Present mainly in tissues enriched in stratified squamous keratinizing epithelium.

Similarity : Belongs to the 14-3-3 family.

Database links : UniProtKB/Swiss-Prot: P31947.1

实验流程：

正在热销的相关产品：

Beta-DF ELISA Kit 大鼠β-防御素Multi-class antibodies规格： 48T

Anti-Phospho-TNK1 (Tyr277) 磷酸化非受体型酪氨酸蛋白激酶Tnk1抗体Multi-class antibodies规格： 0.1ml

Rhesus antibody Rh GLP-1(1G9) GLP-1单克隆抗体 规格 0.1ml

NT-proBNP(Human N-terminal pro-brain natriuretic peptide) ELISA KIT 人N端前脑素 96T

Neuropeptide S 英文名称： 神经肽S抗体 0.2ml

Anthrax 英文名称： 炭疽杆菌抗体 0.2ml

Anti-Phospho-TNK1 (Tyr277) 磷酸化非受体型酪氨酸蛋白激酶Tnk1抗体Multi-class antibodies规格： 0.1ml

Anti-Ezrin/Radixin/FITC 荧光素标记埃兹蛋白抗体IgGMulti-class antibodies规格： 0.2ml

Anti-NAP1 /FITC 荧光素标记核小体组装蛋白1抗体IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh C17orf59 17号染色体开放阅读框59抗体 规格 0.2ml

BRCA1(Breast cancer susceptbility gene 1)(mouse rat) 癌易感基因1抗原(小鼠 大鼠) 0.5mg

HRP2 英文名称： 衍生生长因子2抗体 0.2ml

Rhesus antibody Rh ROC1/RNF75 环指蛋白75抗体 规格 0.2ml

Anti-NAP1 /FITC 荧光素标记核小体组装蛋白1抗体IgGMulti-class antibodies规格： 0.2ml

ODC(Human Ornithine decarboxylase) ELISA Kit 人鸟氨酸脱羧酶Multi-class antibodies规格： 48T

Anti-NOS-2/iNOS 合成酶-2抗体(诱导型)Multi-class antibodies规格： 0.1ml

Rhesus antibody Rh IKK gamma/IKBKG KB抑制蛋白激酶γ抗体 规格 0.2ml

LFA-3/CD58(Mouse lymphocyte function associated antigen 3) ELISA Kit 小鼠淋巴细胞功能相关抗原3 96T

phospho-PIK3R2(Tyr467) 英文名称： 磷酸化磷脂酰肌醇激酶p85β抗体 0.1ml

Connexin-40 英文名称： 间隙连接蛋白40抗体 0.1ml

Anti-NOS-2/iNOS 合成酶-2抗体(诱导型)Multi-class antibodies规格： 0.1ml

FCGR2A Others Human 人 CD32a / FCGR2A CHO细胞裂解液 (阳性对照)

CL-0058CCRF-CEM(人急性淋巴细胞)5×106cells/瓶×2

TUNEL色标法细胞凋亡检测试剂盒TUNEL

牛肾细胞;MDBK(BVDV-free) 鼠胚胎成纤维细胞，PA317细胞 MLA-144细胞，长臂猿

EB病毒转化的人B淋巴细胞(拉祜族);KM9406

ELANE Others Mouse 小鼠 Neuophil Elastase / ELANE / ELA2 人细胞裂解液 (阳性对照)

CL-0345HB611(人细胞)5×106cells/瓶×2

MFI2 Others Mouse 小鼠 MFI2 / CD228 / melanoansferrin 人细胞裂解液 (阳性对照)

小鼠肺大动脉平滑肌细胞完全培养基 100mL

C3H/10T1/2, Clone 8小鼠胚胎成纤维细胞 C3H/10T1/2, Clone 8 mouse embryonic fibroblast MEM培养基(GIBCO)+10%FBS

IL1B Protein Cynomolgus 重组食蟹猴 IL-1 beta / IL1B 蛋白

NCI-H520(人肺鳞癌细胞) 5×106cells/瓶×2 T/G HA-VSMC(人主动脉平滑肌细胞)

14-3-3σ抗体说明书 CL-0342H4-II-E-C3(大鼠细胞 )5×106cells/瓶×2

PDGFRB Others Human 人 PDGFRB / PDGFR-1 / CD140b 人细胞裂解液 (阳性对照)

人肺微内皮细胞总RNAHPMEC NA

HCC1937细胞，人癌细胞 鼠胸腺激酶缺陷细胞株,L-MTK细胞 原代细胞特制基础无血清培养基Many types of cells包装：500/100ml

M-NFS-60 (小鼠细胞G-CSF依赖性) 5×106cells/瓶×2

HDBEC adult Pellet 人皮肤内皮细胞团块(成年捐献者) > 1 mio.cells 间充质干细胞软骨细胞分化培养基MCDM-prf

技术外包服务:

★14-3-3σ抗体说明书 分子生物学：质粒抽提、PCR、Q-PCR、RT-PCR、分子生物学：基因合成、引物合成、基因测序、载体构建等

★蛋白工程：原核、哺乳动物蛋白表达系统等

★病毒包装：腺病毒、慢病毒等

★抗体工程：磁珠分选、病理染色、WB、ELISA、IP、IF、IHC、FACS、Confocal等等

★细胞工程：细胞表型分析(凋亡、增殖、周期、迁移、侵袭、修复、克隆形成)、细胞培养、细胞膜制备、稳定细胞株构建、细胞RNAi技术等等。