产品订购信息：
英文名称  Anti-UNC5H3/UNC5C 

中文名称   神经突起生长诱导因子受体UNC5B抗体品牌 

别    名  Alternative nameshomolog of C. elegans transmembrane receptor Unc5; Netrin receptor UNC5C; Protein unc 5 homolog C; Unc 5 homolog 3; Unc 5 homolog C; Unc5 (C.elegans homolog) c; UNC5H3; UNC5C_HUMAN.

浓    度   1mg/1ml

规 格  0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep, 1 

产品类型   一抗  

研究领域    细胞生物 发育生物学 神经生物学

蛋白分子量  predicted molecular weight: 99kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human UNC5H3 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

神经突起生长诱导因子受体UNC5B抗体品牌 产品应用    ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:100-500 ICC=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 The UNC5H family of proteins act as transmembrane receptors for netrin-1 and play a crucial role in axon guidance and migration of neural cells. Additionally, UNC5H receptors induce apoptosis when cleaved by a caspase, producing an intracellular fragment containing a death domain. This activity is blocked by the binding of netrin-1. In the absence of netrin-1, UNC5H receptors act as tumor suppressors by inhibiting anchorage-independent growth and invasion, but mutation of these receptors provides a potential mechanism for tumorigenicity. The expression of UNC5H receptors is down-regulated in multiple cancers, including colorectal, breast, ovary, uterus, stomach, lung, and kidney cancers. UNC5H3, also known as UNC5C, plays an important role in the development of spinal accessory motor neurons. It is also involved in mediating the repulsive action for netrin-1 and it serves as a stop signal for migratory cells.

Function : UNC5C is a receptor for netrin required for axon guidance. It mediates axon repulsion of neuronal growth cones in the developing nervous system upon ligand binding. Axon repulsion in growth cones may be caused by its association with DCC that may trigger signaling for repulsion. It is also involved in corticospinal tract axon guidances independently of DCC. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand. There are two named isoforms.

Subunit : Interacts with the cytoplasmic part of DCC

Subcellular Location : Membrane; Single-pass type I membrane protein

Tissue Specificity : Mainly expressed in brain. Also expressed in kidney. Not expressed in developing or adult lung.

Post-translational modifications : Phosphorylated on different cytoplasmic tyrosine residues. Phosphorylation of Tyr-568 leads to an interaction with PTPN11 phosphatase, suggesting that its activity is regulated by phosphorylation/dephosphorylation. Tyrosine phosphorylation is netrin-dependent (By similarity).

Proteolytically cleaved by caspases during apoptosis. The cleavage does not take place when the receptor is associated with netrin ligand. Its cleavage by caspases is required to induce apoptosis

Similarity : Belongs to the unc-5 family.

Contains 1 death domain.

Contains 1 Ig-like (immunoglobulin-like) domain.

Contains 1 Ig-like C2-type (immunoglobulin-like) domain. [SIMILARITY] Contains 2 TSP type-1 domains. [SIMILARITY] Contains 1 ZU5 domain.

Database links : UniProtKB/Swiss-Prot: O95185.2

Parvalbumin 微白蛋白/细小清蛋白抗原Multi-class antibodies规格： 0.5mg

Anti-BADH2 BADH2抗体(水稻)Multi-class antibodies规格： 0.2ml

Rhesus antibody Rh Phospho-5-Lipoxygenase(Ser663) 磷酸化5-脂氧合酶抗体 规格 0.1ml

Keratin19 浓缩液 0.1ml 进口分装

UBE2A 英文名称： 泛素结合酶E2蛋白A抗体 0.2ml

CHST10 英文名称： 碳水化合物磺基转移酶10抗体 0.1ml

Anti-BADH2 BADH2抗体(水稻)Multi-class antibodies规格： 0.2ml

ASK1/MAPKKK5 细胞凋亡信号调节激酶1/原活化蛋白激酶激酶激酶5抗原Multi-class antibodies规格： 0.5mg

Anti-AMPK alpha 1 腺苷单磷酸活化蛋白激酶α1抗体Multi-class antibodies规格： 0.1ml

Rhesus antibody Rh Phospho-BRCA1(Ser1189) 磷酸化癌易感基因1抗体 规格 0.1ml

Tris-EDTA缓冲液(50X) 50ml pH 9.0,高背景着色

VASP 英文名称： 扩张刺激磷蛋白抗体 0.1ml

Defensin alpha 6 英文名称： 防御素α6抗体 0.2ml

Anti-AMPK alpha 1 腺苷单磷酸活化蛋白激酶α1抗体Multi-class antibodies规格： 0.1ml

Anti-Phospho-SHIP2 (Ser576) /FITC 荧光素标记磷酸化蛋白酪氨酸磷酸酶2抗体IgGMulti-class antibodies规格： 0.2ml

AATK peptide 细胞凋亡关联酪氨酸激酶抗原Multi-class antibodies规格： 0.5mg

神经激肽B受体抗体 Anti-NKR 0.1ml

Somatostatin 28 英文名称： 生长抑素抗体 0.1ml

Endothelin 2 英文名称： 内皮素2抗体 0.1ml

Rhesus antibody Rh Phospho-p56Dok2(Tyr351) 磷酸化D酪氨酸激酶衰减蛋白2抗体 规格 0.1ml

AATK peptide 细胞凋亡关联酪氨酸激酶抗原Multi-class antibodies规格： 0.5mg

大鼠可溶性内皮细胞蛋白C受体(sEPCR)ELISA试剂盒 ，英文名： sEPCR ELISA Kit

人内皮细胞生长因子受体1(VEGFR-1)ELISA检测试剂盒HumanVascuoarendothelialcellgrowthfactorreceptor1,VEGFR-1ELISAkit 96T/48T

三聚胺ELISA快速检测试剂盒 Melamine ELISA Kit

CLIAKitforOPG(HumanOsteoprotegerin)ELISAKIT人骨保护素规格：48T/96T

糖化血清白蛋白(GSP)化学比色法定量检测试剂盒50次

ELISAKitAO人橙规格：48T/96T

大鼠白细胞介素1α(IL-1α)ELISA试剂盒 ，英文名： IL-1α ELISA Kit

Lens culinaris agglinin (LCA) ELISA Kit 扁豆凝集素(LCA)ELISA试剂盒

Mousegrowthhormonereleasingpeptide-Ghrelin,GHRP-GhrelinELISAKit 小鼠生长激素释放肽ghrelin(GHRP-Ghrelin)ELISA试剂盒 96T/48T 进口分装

CLIAKitforHumanNon-PhosphorylatedInsulin-likegrowthfactorbindingprotein1ELISAKit人未磷酸化类生长因子结合蛋白-1规格：48T/96T

同位素真菌/酵母细胞蛋白核酸结合凝胶迁移电泳分析试剂盒20次

ELISAKitsICAM-1大鼠可溶性细胞间粘附分子1规格：48T/96T

神经突起生长诱导因子受体UNC5B抗体品牌 大鼠催乳素诱导蛋白(PIP)ELISA试剂盒 ，英文名： PIP ELISA Kit

Rat ierleukin 9 (IL-9) ELISA Kit 大鼠白介素9(IL-9)ELISA试剂盒

RatProstaglandinE2,PG-E2ELISAKit 大鼠素E2(PGE2)ELISA试剂盒 96T/48T 进口分装

CLIAKitforCTLA-4/CD152(HumancytotoxicTlymphocyteassociatedaigen4)ELISAKit人细胞毒性T淋巴细胞相关抗原4规格：48T/96T

细胞糖原化学水解比色法定量检测试剂盒20次

Rateosinophilcationicprotein,ECPELISAKit大鼠嗜酸性粒细胞阳离子蛋白(ECP)ELISA试剂盒规格：96T/48T

抗体的生物素化标记实验要点：

1. 神经突起生长诱导因子受体UNC5B抗体品牌 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及苯酚基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

抗体的鉴定：

1）神经突起生长诱导因子受体UNC5B抗体品牌 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。