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泛素激活酶2（泛素激活酶E1相关蛋白抗体）说明书 全：公司提供上万种产品，涵盖了生物试剂，elisa试剂盒，标准品，培养基，原装耗材，抗体、培养基、ATCC细胞等，基本上各种科研所需产品在我司都能找到。

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泛素激活酶2（泛素激活酶E1相关蛋白抗体）说明书 英文名称  Anti-Ube1L/UBE2

中文名称  泛素激活酶2（泛素激活酶E1相关蛋白抗体）说明书 

别    名 D8; UBA7; Ube 1L; UBE 2; UBE2; Ubiquitin activating enzyme 2; Ubiquitin activating enzyme E1 homolog; Ubiquitin Activating Enzyme E1 Like; Ubiquitin activating enzyme E1 related protein; UBA7_HUMAN.

产品属性：

浓    度  1mg/1ml

规 格  0.2ml/200μg

抗体来源  Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Pig, Cow, Horse, Rabbit

产品类型   一抗  

研究领域    细胞生物 免疫学 信号转导 细胞凋亡 转录调节因子

蛋白分子量  predicted molecular weight: 112kDa 

性    状   Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from human Ube1L

亚    型  IgG

纯化方法   affinity purified by Protein A

储 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

泛素激活酶2（泛素激活酶E1相关蛋白抗体）说明书 产品应用   IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme is a retinoid target that triggers promyelocytic leukemia (PML)/retinoic acid receptor alpha (RARalpha) degradation and apoptosis in acute promyelocytic leukemia, where it is involved in the conjugation of the ubiquitin-like interferon-stimulated gene 15 protein. [provided by RefSeq, Jul 2008].

Function : Activates ubiquitin by first adenylating with ATP its C-terminal glycine residue and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP.

Subunit : Monomer (By similarity). Binds and is involved in the conjugation of G1P2/ISG15.

Tissue Specificity : Expressed in a variety of normal and tumor cell types, but is reduced in lung cancer cell lines.

Similarity : Belongs to the ubiquitin-activating E1 family.

Database links : UniProtKB/Swiss-Prot: P41226.2

实验流程：

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★泛素激活酶2（泛素激活酶E1相关蛋白抗体）说明书 分子生物学：质粒抽提、PCR、Q-PCR、RT-PCR、分子生物学：基因合成、引物合成、基因测序、载体构建等

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