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微管蛋白β5抗体说明书 英文名称  Anti-TUBB5/Beta V Tubulin

中文名称  微管蛋白β5抗体说明书 

别    名 beta 4 tubulin; Beta 5 tubulin; beta Ib tubulin; M40; TUBB1; TUBB5; tubulin beta 1 chain; Tubulin beta 4 chain; tubulin beta 5 chain; Tubulin beta; Tubulin beta chain; tubulin beta polypeptide; TBB5_HUMAN.

产品属性：

浓    度  1mg/1ml

规 格  0.2ml/200μg

抗体来源  Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Zebrafish, Sheep, Xenopus laevis

产品类型   一抗  

研究领域    细胞生物 信号转导 细胞骨架

蛋白分子量  predicted molecular weight: 50kDa 

性    状   Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from human TUBB5/Beta V Tubulin

亚    型  IgG

纯化方法   affinity purified by Protein A

储 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

微管蛋白β5抗体说明书 产品应用   WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a nonexchangeable site on the alpha chain. The highly acidic C terminal region may bind cations such as calcium.

Function : Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

Subunit : Dimer of alpha and beta chains. May interact with RNABP10 (By similarity). Interacts with PIFO. Interacts with MX1 (By similarity).

Subcellular Location : Cytoplasmic, cytoskeleton.

Tissue Specificity : Ubiquitously expressed with highest levels in spleen, thymus and immature brain.

Post-translational modifications : Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules (Probable).

Similarity : Belongs to the tubulin family.

Database links : UniProtKB/Swiss-Prot: P07437.2

实验流程：

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