抗体的生物素化标记实验要点：

1. 转录因子7类似物2抗体规格 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及苯酚基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

产品订购信息：
英文名称  Anti-TCF7L2 

中文名称   转录因子7类似物2抗体规格 

别    名  E2 2; HMG box transcription factor 4; hTCF 4; Immunoglobulin transcription factor 2; ITF2; SEF2 1; SEF2 1A; SEF2 1B; SEF2; T cell factor 4; T cell specific HMG box; T cell specific transcription factor 4; TCF 4; TCF4; TCF7L2; TCF7L2 protein; hTCF-4; T-cell factor 4; T-cell-specific transcription factor 4; TCF-4; TF7L2_HUMAN; Transcription factor 7-like 2; Transcription factor 4; Transcription factor 7 like 2; Transcription factor 7 like 2 T cell specific HMG box.

浓    度   1mg/1ml

规 格  0.1ml/100μg 0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Chicken, Dog, Pig, Cow 

产品类型   一抗  

研究领域    心 染色质和核信号 转录调节因子 表观遗传学

蛋白分子量  predicted molecular weight: 68kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human TCF7L2 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

转录因子7类似物2抗体规格 产品应用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 TCF-4, transcription factor 4, is a basic helix-turn-helix transcription factor. This protein recognizes an Ephrussi-box ('E-box') binding site ('CANNTG') - a motif first identified in immunoglobulin enhancers. The gene for TCF-4 is expressed predominantly in pre-B-cells, although it is found in other tissues as well. Multiple alternatively spliced transcript variants that encode different proteins have been described. TCF4, also known as TCF7L2, is expressed widely during development. Gene targeting study indicates that it is required to maintain the crypt stem cells of the small intestine. TCF4 has many different splicing isoforms and they are expressed differentially in tissues and in cancers of different stages. Studies also indicate that variant of the TCF4 gene confers an increased risk of type 2 diabetes.

Function : Participates in the Wnt signaling pathway and modulates MYC expression by binding to its promoter in a sequence-specific manner. Acts as repressor in the absence of CTNNB1, and as activator in its presence. Activates transcription from promoters with several copies of the Tcf motif 5'-CCTTTGATC-3' in the presence of CTNNB1. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7L2/TCF4 and CTNNB1. Expression of dominant-negative mutants results in cell-cycle arrest in G1. Necessary for the maintenance of the epithelial stem-cell compartment of the small intestine.

Subunit : Interacts with TGFB1I1 (By similarity). Interacts with CTNNB1 (via the armadillo repeat); forms stable transcription complex. Interacts with EP300. Interacts with NLK. Interacts with CCDC85B (probably through the HMG box); prevents interaction with CTNNB1. Interacts with TNIK. Interacts with MAD2L2; prevents TCF7L2/TCF4 binding to promZIPK/DAPK3oters, negatively modulating its transcriptional activity. Interacts with ZIPK/DAPK3. Interacts with XIAP/BIRC4 and TLE3. Interacts with DDIT3/CHOP.

Subcellular Location : Nucleus, PML body. Note=Diffuse pattern. Colocalizes with SUMO1 and PIAS4 in a subset of PML (promyelocytic leukemia) nuclear bodies.

Tissue Specificity : Detected in epithelium from small intestine, with the highest expression at the top of the crypts and a gradient of expression from crypt to villus. Detected in colon epithelium and colon cancer, and in epithelium from mammary gland and carcinomas derived therefrom.

Post-translational modifications : In vitro, phosphorylated by TNIK.

Phosphorylated at Thr-201 and/or Thr-212 by NLK. Phosphorylation by NLK at these sites inhibits DNA-binding by TCF7L2/TCF4, thereby preventing transcriptional activation of target genes of the canonical Wnt/beta-catenin signaling pathway.

Polysumoylated. Sumoylation is enhanced by PIAS family members and desumoylation is enhanced by SENP2. Sumoylation/desumoylation regulates TCF7L2/TCF4 transcription activity in the Wnt/beta-catenin signaling pathway without altering interaction with CTNNB1 nor binding to DNA.

DISEASE : Note=Constitutive activation and subsequent transactivation of target genes may lead to the maintenance of stem-cell characteristics (cycling and longevity) in cells that should normally undergo terminal differentiation and constitute the primary transforming event in colorectal cancer (CRC).

Diabetes mellitus, non-insulin-dependent (NIDDM) [MIM:125853]: A multifactorial disorder of glucose homeostasis caused by a lack of sensitivity to the body's own insulin. Affected individuals usually have an obese body habitus and manifestations of a metabolic syndrome characterized by diabetes, insulin resistance, hypertension and hypertriglyceridemia. The disease results in long-term complications that affect the eyes, kidneys, nerves, and blood vessels. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.

Similarity : Belongs to the TCF/LEF family.

Contains 1 HMG box DNA-binding domain.

Database links : UniProtKB/Swiss-Prot: Q9NQB0.2

主要用于的研究，TCF7L2基因可能存在双重效应，抑制β细胞代偿，减小分泌的退化。

抗体的鉴定：

1）转录因子7类似物2抗体规格 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。

GABA/BSA γ1氨基偶联血清白蛋白Multi-class antibodies规格： 1mg

Anti-HER4/ErbB4 HER4抗体Multi-class antibodies规格： 0.2ml

Rhesus antibody Rh OAT-1/SLC22A6 阴离子转运蛋白-1抗体 规格 0.1ml

HRP/Horseradish Peroxidase 辣根过氧化物酶(辣根酶) 10mg

TRP2 英文名称： 酪酸酶相关蛋白2抗体 0.2ml

c-Maf 英文名称： 原癌基因cMAF抗体 0.2ml

Anti-HER4/ErbB4 HER4抗体Multi-class antibodies规格： 0.2ml

cAMP ELISA Kit 大鼠环磷酸腺苷Multi-class antibodies规格： 48T

Anti-ARC/FITC 荧光素标记ARC抗体IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh FAM154A FAM154A蛋白抗体 规格 0.2ml

E-Cad(Mouse E-Cadherin) ELISA Kit 小鼠E钙粘着蛋白/上皮性钙黏附蛋白 96T

Angiotensin II Type 2 Receptor 英文名称： 紧张素Ⅱ2型受体相互作用蛋白抗体 0.1ml

Annexin A11 英文名称： 膜粘连蛋白11抗体 0.2ml

Anti-ARC/FITC 荧光素标记ARC抗体IgGMulti-class antibodies规格： 0.2ml

Anti-STEAP1/FITC 荧光素标记跨膜上皮抗原1抗体IgGMulti-class antibodies规格： 0.2ml

GIP (Gastric Inhibitory polypeptide) 胃泌素抑制肽(抗原)Multi-class antibodies规格： 0.5mg

人胎盘生长因子相关抗体 Anti-PLGF 0.1ml

Phospho-Stathmin (Ser16) 英文名称： 磷酸化原癌基因蛋白18抗体 0.1ml

E-Selectin 英文名称： E选择素抗体 0.1ml

Rhesus antibody Rh Phospho-mTOR (Ser2481) 磷酸化雷帕霉素靶蛋白抗体 规格 0.1ml

GIP (Gastric Inhibitory polypeptide) 胃泌素抑制肽(抗原)Multi-class antibodies规格： 0.5mg

大鼠内毒素(Endotoxin)ELISA试剂盒 ，英文名： Endotoxin ELISA Kit

人白介素1β(IL-1β)ELISA检测试剂盒HumanIerleukin1β,IL-1βELISAKit 96T/48T

鱼17-羟孕烯醇酮免疫试剂盒 Fish 17-hydroxypregnenolone ELISA kit

CLIAKitforPAP(MouseProstaticAcidPhosphatase)ELISAKit小鼠酸性磷酸酶规格：48T/96T

水溶性四氮唑-8比色法细胞繁殖测定试剂盒500次

ELISAKitmIgA人表面膜免疫球蛋白A规格：48T/96T

大鼠成纤维细胞生长因子11(FGF11)ELISA试剂盒 ，英文名： FGF11 ELISA Kit

Human XC chemokine receptor 1 (XCR1) ELISA Kit 人XC趋化因子受体1(XCR1)ELISA试剂盒

RatCarboxyterminalpropeptideoftypeⅠprocollagen,PⅠCPELISAKit 大鼠Ⅰ型前胶原羧基端肽(PⅠCP)ELISA试剂盒 96T/48T 进口分装

CLIAKitforCr(MouseCrosslaps)ELISAKit小鼠骨胶原交联规格：48T/96T

细胞紧张素Ⅰ转化酶2(ACE2)活性荧光共振能量转移法(FRET)定量检测试剂盒20次

RatCystatinC,Cys-CELISAKit大鼠半胱氨酸蛋白酶抑制剂/胱抑素C(Cys-C)ELISA试剂盒规格：96T/48T

转录因子7类似物2抗体规格 大鼠端粒酶(TE)ELISA试剂盒 ，英文名： TE ELISA Kit

Porcine growth hormone (GH) ELISA Kit 猪生长激素(GH)ELISA试剂盒

植物源性成分(RBCL)核酸检测试剂盒(PCR-荧光探针法) 48T

CLIAKitforLTC4ELISAKit大鼠白三烯C4规格：48T/96T

体液总脂蛋白胆固醇(TotalL-Cholesterol)酶连续循环比色法定量检测试剂盒20次

ELISAKitHSP-70鸡热休克蛋白70规格：48T/96T