DNA拓普西异构酶Ⅱ抗体规格 英文名称  Anti-TOPO II/TOPO II Alpha

中文名称  DNA拓普西异构酶Ⅱ抗体规格 

别    名 DNA topoisomeraseⅡalpha; DNA topoisomerase 2-alpha; DNA topoisomerase II, alpha isozyme; TOP2; TP2A; TOP2A; DNA topoisomerase II 170 kD; DNA topoisomerase II alpha isozyme; DNA Topoisomerase2; TOP 2A; TopII alpha; Topo II alpha; Topoisomerase DNA II alpha 170kDa; Topoisomerase II alpha 170 kDa; TOP2A_HUMAN.

产品属性：

浓    度  1mg/1ml

规 格  0.1ml/100μg 0.2ml/200μg

抗体来源  Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat

产品类型   一抗  

研究领域     转录调节因子 激酶和磷酸酶

蛋白分子量  predicted molecular weight: 174kDa 

性    状   Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from human TOPO II alpha

亚    型  IgG

纯化方法   affinity purified by Protein A

储 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

DNA拓普西异构酶Ⅱ抗体规格 产品应用   WB=1:100-500 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromsome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. [provided by RefSeq].

Function : Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Essential during mitosis and meiosis for proper segregation of daughter chromosomes.

Subunit : Homodimer. Interacts with COPS5.

Subcellular Location : Cytoplasm. Nucleus, nucleoplasm. Note=Generally located in the nucleoplasm.

Post-translational modifications : Phosphorylation has no effect on catalytic activity. However, phosphorylation at Ser-1106 by CSNK1D/CK1 promotes DNA cleavable complex formation.

Similarity : Belongs to the type II topoisomerase family.

Contains 1 Toprim domain.

Database links : UniProtKB/Swiss-Prot: P11388.3

拓扑异构酶II是一种能催化DNA 拓扑异构体相互变换，调节DNA三维结构的核酶。与染色质或染色体的组装与分组装、基因组复制、DNA重组、基因转录及DNA损伤后修复等有密切的关系。许多人的都有拓扑异构酶II的过表达，因此，拓扑异构酶II是许多药的靶标。大部分以拓扑异构酶II为重要靶标的药物是通过稳定的DNA与拓扑异构酶II的复合物激发凋亡的。

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