产品订购信息：
英文名称  Anti-TOP2 alpha 

中文名称   DNA拓普西异构酶ⅡA抗体品牌 

别    名  Topoisomerase Ⅱalpha; DNA topoisomerase II, alpha isozyme; DNA topoisomerase 2 alpha; DNA topoisomerase II 170 kD; DNA Topoisomerase2; TOP2; TOP2A; TopII alpha; Topo II alpha; Topoisomerase DNA II alpha 170kDa; Topoisomerase II alpha 170 kDa; TP2A; TPⅡA; TOP2A_HUMAN.

浓    度   1mg/1ml

规 格  0.1ml/100μg 0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit 

产品类型   一抗  

研究领域     染色质和核信号 信号转导 转录调节因子

蛋白分子量  predicted molecular weight: 174kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human TOP2 alpha (592-643aa) 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

DNA拓普西异构酶ⅡA抗体品牌 产品应用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromsome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia.

Function : Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks.

Subunit : Homodimer. Interacts with COPS5.

Subcellular Location : Cytoplasm. Nucleus, nucleoplasm. Note=Generally located in the nucleoplasm.

Post-translational modifications : Phosphorylation has no effect on catalytic activity. However, phosphorylation at Ser-1106 by CSNK1D/CK1 promotes DNA cleavable complex formation.

Similarity : Belongs to the type II topoisomerase family.

Database links : UniProtKB/Swiss-Prot: P11388.3

Eotaxin-2 human 人噬酸性粒细胞趋化因子-2Multi-class antibodies

Anti-phospho C-Met/HGFR(Tyr1365) 磷酸化原癌基因c-Met抗体Multi-class antibodies规格： 0.1ml

Rhesus antibody Rh NPC1/Niemann Pick C1 尼曼匹克C1前体蛋白抗体 规格 0.2ml

Col I (Human Collagen Type I ) ELISA Kit 人Ⅰ型胶原 96T

TPX2 英文名称： 微管相关同源蛋白TPX2抗体 0.2ml

C6orf225 英文名称： 6号染色体开放阅读框225抗体 0.2ml

Anti-phospho C-Met/HGFR(Tyr1365) 磷酸化原癌基因c-Met抗体Multi-class antibodies规格： 0.1ml

PEPT2 (Peptide-transporters 2) 肠道肽转运蛋白2/小肽转运蛋白2抗原Multi-class antibodies规格： 0.5mg

Anti-ATP1b2/Na+K+ATPase ATP酶通道蛋白抗体Multi-class antibodies规格： 0.2ml

Rhesus antibody Rh phospho-Alpha synuclein(Tyr133) 磷酸化核突触蛋白α抗体 规格 0.1ml

Ostepontin 浓缩液 0.1ml 进口分装

Urocortin 英文名称： 尿皮质素抗体 0.2ml

DEDD1 英文名称： 死亡效应结构域蛋白1抗体 0.2ml

Anti-ATP1b2/Na+K+ATPase ATP酶通道蛋白抗体Multi-class antibodies规格： 0.2ml

Anti-PTPRJ/CD148/DEP1/FITC 荧光素标记CD148抗体IgGMulti-class antibodies规格： 0.2ml

Recomb Protein A 重组蛋白AMulti-class antibodies规格： 1mg

粘附分子整合素α5抗体 Anti-ITG-α5/CD49e 0.2ml

NTR3 英文名称： 神经降压素受体3抗体(糖蛋白95) 0.2ml

FAAH1 英文名称： 脂肪酸酰胺水解酶1抗体 0.2ml

Rhesus antibody Rh Phospho-PPIG (Ser376) 磷酸化亲环蛋白(亲环素)PPIG抗体 规格 0.2ml

Recomb Protein A 重组蛋白AMulti-class antibodies规格： 1mg

大鼠凝血因子Ⅹ(FⅩ)ELISA 试剂盒 96T/48T 试剂盒 组装/原装

人丝氨酸/苏氨酸激酶24(STK24)免疫试剂盒 Human Serine/threonine-protein kinase 24,STK24 ELISA Kit

果胶含量试剂盒 可见分光光度法 50管/24样

RatVascularEndothelialcellGrowthFactor,VEGFELISA试剂盒大鼠内皮细胞生长因子(VEGF)ELISA试剂盒规格：96T/48T

humanlymphocyteaigen6complexlocusA,Ly6aELISA试剂盒人淋巴细胞抗原6复合物基因座A(Ly6a)ELISA试剂盒规格：96T/48T

HumanparamyxovirusparotitisIgGELISAKit人病毒IgGELISA试剂盒规格：96T/48T

大鼠激素释放激素受体(GHR)ELISA试剂盒 ，英文名： GHR ELISA Kit

Porcine histamine (HIS) ELISA Kit 猪组胺(HIS)ELISA试剂盒

ELISA 小鼠脑衍化神经营养因子(mouse BDNF) 48T/96T 进口分装

CLIAKitforL-LDH(HumanL-LactateDehydrogenase)ELISAKit人L-乳酸脱氢酶规格：48T/96T

通用型EBV(EPSTEIN-BARR)病毒定量PCR扩增检测试剂盒20次

ELISAKitCRP鸡C反应蛋白规格：48T/96T

DNA拓普西异构酶ⅡA抗体品牌 大鼠肺表面活性物质相关蛋白C(SP-C)ELISA 试剂盒 96T/48T 试剂盒 组装/原装

人鸟苷酸交换因子(GEF)免疫试剂盒 Human Guanine Exchange Factor,GEF ELISA Kit

Mousesexhormone-bindingglobulin,SHBGELISAKit小鼠性激素结合球蛋白(SHBG)ELISA试剂盒规格：96T/48T

冰冻切片组织RHO蛋白表达NBT显色光学显微镜检测试剂盒10/20次

HumanPlacealalkalinepkosphatase,PLAPELISA试剂盒人胎盘碱性磷酸酶(PLAP)ELISA试剂盒规格：96T/48T

Humansolubletumornecrosisfactor-relatedapoptosisinducingligand,sAILELISAKit人可溶性坏死因子相关凋亡诱导配体(sAIL)ELISA试剂盒规格：96T/48T

抗体的生物素化标记实验要点：

1. DNA拓普西异构酶ⅡA抗体品牌 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及苯酚基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

抗体的鉴定：

1）DNA拓普西异构酶ⅡA抗体品牌 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。