产品订购信息：
英文名称  Anti-TROVE-2/SSA2 

中文名称   相关蛋白2抗体说明书 

别    名  TROVE2; 60 kDa ribonucleoprotein Ro; 60 kDa Ro protein; 60 kDa SS A/Ro ribonucleoprotein; 60 kDa SS-A/Ro ribonucleoprotein; Autoantigen Ro/SSA, 60-KD; bA101E13.2; Gastric cancer multi drug resistance protein; Ribonucleoprotein autoantigen SS A/Ro; RO 60; Ro 60 kDa autoantigen; RO60; RO60_HUMAN; RoRNP; Sjoegren syndrome antigen A2; Sjoegren syndrome type A antigen; Sjogren syndrome antigen A2 (60kD ribonucleoprotein autoantigen SS A/Ro); Sjogren syndrome antigen A2 (60kDa ribonucleoprotein autoantigen SS A/Ro); Sjogren syndrome antigen A2; SS A; SS-A; SS-A/Ro; SSA 2; SSA; SSA2; TROVE 2; TROVE domain family member 2; TROVE2.

浓    度   1mg/1ml

规 格  0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Sheep 

产品类型   一抗  

研究领域    细胞生物 免疫学 表观遗传学

蛋白分子量  predicted molecular weight: 61kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human TROVE-2/SSA2 (351-450aa) 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

相关蛋白2抗体说明书 产品应用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 Ro autoantigens are of clinical significance because antibodies directed against them are found in most patients with primary Sjqgren syndrome, subacute cutaneous lupus erythematosus (SLE), neonatal lupus erythematosus, ANA-negative lupus erythematosus, and systemic lupus erythematosus-like disease secondary to homozygous C2 or C4 complement deficiency (1). Ro/SSA is a ribonucleoprotein that binds to autoantibodies in 35 to 50% of patients with SLE and in up to 97% of patients with Sjqgren syndrome (2). The Ro/SSA particle consists of a single immunoreactive protein noncovalently bound with one of four small RNA molecules (2). Most anti-Ro/SSA-positive sera antibodies detect not only the main protein, but also a smaller Ro/SSA protein (2). The genes which encode the smaller and larger proteins map to human chromosomes 11p15.5 and 1q31, respectively (3?). La/SSB is an autoimmune RNA-binding protein that plays a role in the transcription of RNA polymerase III was originally defined by its reactivity with autoantibodies from patients with Sj鰃ren syndrome and SLE (6).

Function : RNA-binding protein that binds to several small cytoplasmic RNA molecules known as Y RNAs. May stabilize these RNAs from degradation.

Subunit : Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Found in a complex with PUF60 and Y5 RNA. Interacts with RIP11.

Subcellular Location : Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.

Post-translational modifications : Phosphorylated upon DNA damage, probably by ATM or ATR.

Similarity : Belongs to the Ro 60 kDa family. Contains 1 TROVE domain.

Database links : UniProtKB/Swiss-Prot: P10155.2

Anti-Phospho-mu Opioid Receptor (Ser375) /FITC 荧光素标记磷酸化μ-型受体抗体IgGMulti-class antibodies规格： 0.2ml

Rabbit Anti-mouse IgM/HRP 辣根过氧化物酶标记兔抗小鼠IgMMulti-class antibodies规格： 0.1ml

c-erbB-4癌基因抗体 Anti-C-erbB-4/HER4/erbB-4 0.1ml

Rabbit Anti-horse IgM/RBITC 罗丹明标记的兔抗马IgM 0.1ml

FGF14 英文名称： 成纤维细胞生长因子14抗体 0.2ml

Rhesus antibody Rh PKC-γ/SCA14 蛋白激酶C亚型G抗体 规格 0.2ml

Rabbit Anti-mouse IgM/HRP 辣根过氧化物酶标记兔抗小鼠IgMMulti-class antibodies规格： 0.1ml

HSP47(Heat shock protein-47) 热休克蛋白-47抗原Multi-class antibodies规格： 0.5mg

Anti-phospho-c-Abl(Tyr245) 磷酸化非受体酪氨酸激酶c-Abl抗体Multi-class antibodies规格： 0.1ml

Rhesus antibody Rh Phospho-CHEK1 (Ser296) 磷酸化细胞周期检测点激酶1抗体 规格 0.1ml

兔抗人IgG-F(ab)2 0.5ml 国产

WD SOCS box protein 2 英文名称： 信号传导抑制蛋白WD重复蛋白2抗体 0.2ml

Phospho-Dab1 (Tyr220) 英文名称： 磷酸化Disabled 1抗体 0.1ml

Anti-phospho-c-Abl(Tyr245) 磷酸化非受体酪氨酸激酶c-Abl抗体Multi-class antibodies规格： 0.1ml

Anti-AFP/Biotin 生物素化鼠抗人甲胎蛋白单克隆抗体(检测)IgGMulti-class antibodies规格： 0.2ml

E2F1 转录因子E2F-1Multi-class antibodies规格： 0.5mg

磷酸化APP(Tyr757)淀粉样肽前体蛋白抗体 Anti-phospho-APP 0.1ml

ZnT-1 英文名称： 锌转运蛋白1抗体 0.2ml

DHRS2 英文名称： 脱氢酶/还原酶家族成员2抗体 0.1ml

Rhesus antibody Rh Phospho-eNOS(Ser1177) 磷酸化合成酶3抗体(内皮型) 规格 0.1ml

E2F1 转录因子E2F-1Multi-class antibodies规格： 0.5mg

人γ肽(Pγ)ELISA 试剂盒 96T/48T 试剂盒 组装/原装

兔子基质金属蛋白酶抑制因子1(TIMP-1)免疫试剂盒 Rabbit tissue inhibitors of metalloproteinase 1,TIMP-1 ELISA Kit

神经酸酶(NA)ELISA试剂盒 ，英文名： NA ELISA Kit

Porcineierleukin4,IL-4ELISA试剂盒猪白介素4(IL-4)ELISA试剂盒规格：96T/48T

Humanaldehydedehydrogenase,ALDHELISA试剂盒人乙脱氢酶(ALDH)ELISA试剂盒规格：96T/48T

HumanApolipoproteinC3，ApoC3ELISAKit人载脂蛋白C3(ApoC3)ELISA试剂盒规格：96T/48T

大鼠水通道蛋白1(AQP-1)ELISA 试剂盒 96T/48T 试剂盒 组装/原装

人铁调素(Hepc)免疫试剂盒 Human hepcidin,Hepc ELISA Kit

中性木聚糖酶测试盒 可见分光光度法 50管/24样

RatsolubleToll-likereceptor6,sTLR-6ELISA试剂盒大鼠可溶性Toll样受体6(sTLR6)ELISA试剂盒规格：96T/48T

HumanKallikrein11,KLK11ELISA试剂盒人激肽释放酶11(KLK11)ELISA试剂盒规格：96T/48T

HumanNein-1,n1ELISAKit人轴突生长诱向因子1(n1)ELISA试剂盒规格：96T/48T

相关蛋白2抗体说明书 大鼠维生素B6(VB6)ELISA试剂盒 ，英文名： VB6 ELISA Kit

人颗粒酶B(Gzms-B)ELISA检测试剂盒HumangranzymesB,Gzms-BELISAKit 96T/48T

仓鼠脂氧素A4(LXA4)免疫试剂盒 Hamster Lipoxin A4,LXA4 ELISA Kit

CLIAKitforRBC(Humanai-redcellaibody)ELISAKit人抗红细胞抗体规格：48T/96T

石蜡切片透明质酸染色试剂盒50次

ELISAKitON人骨粘连蛋白规格：48T/96T

抗体的生物素化标记实验要点：

1. 相关蛋白2抗体说明书 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及苯酚基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

抗体的鉴定：

1）相关蛋白2抗体说明书 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。