抗体的生物素化标记实验要点：

1. 突触融合蛋白结合蛋白5抗体规格 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及苯酚基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

产品订购信息：
英文名称  Anti-Tomosyn/STXBP5 

中文名称   突触融合蛋白结合蛋白5抗体规格 

别    名  FLJ30922; Lethal(2) giant larvae protein homolog 3; LGL3; LLGL3; MGC141942; MGC141968; Nbla04300; STXBP5; Syntaxin binding protein 5; Syntaxin-binding protein 5; Tomosyn 1; STXB5_HUMAN.

浓    度   1mg/1ml

规 格  0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit, Sheep 

产品类型   一抗  

研究领域    细胞生物 神经生物学 细胞凋亡 细胞周期蛋白 结合蛋白

蛋白分子量  predicted molecular weight: 127kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human Tomosyn/STXBP5 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

突触融合蛋白结合蛋白5抗体规格 产品应用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 WD-repeats are motifs that are found in a variety of proteins and are characterized by a conserved core of 40-60 amino acids that commonly form a tertiary propeller structure. While proteins that contain WD-repeats participate in a wide range of cellular functions, they are generally involved in regulatory mechanisms concerning chromatin assembly, cell cycle control, signal transduction, RNA processing, apoptosis and vesicular trafficking. Tomosyn, also known as STXBP5 (syntaxin binding protein 5), LLGL3 or LGL3, is a 1,151 amino acid protein that localizes to the cytoplasm, as well as to the cell junction, secretory vesicles and to the peripheral membrane and contains one v-SNARE coiled-coil homology domain and 14 WD repeats. Interacting with Syntaxin 1 and Syntaxin 1B, Tomosyn functions as a regulator of neurotransmitter release and calcium-dependent exocytosis. Additionally, Tomosyn inhibits membrane fusion and may play a role in the assembly of SNARE complexes between transport vesicles and the plasma membrane. Multiple isoforms of Tomosyn exist due to alternative splicing events.

Function : Tomosyn plays a regulatory role in calcium-dependent exocytosis and neurotransmitter release by inhibiting the assembly of trans-SNARE complexes between transport vesicles and the plasma membrane, via the displacement of munc18 from syntaxin-1. There are three splice variants of tomosyn designated m-tomosyn, b-tomosyn and s-tomosyn. Although b-tomosyn is ubiquitously expressed, s-tomosyn and m-tomosyn are expressed primarily in brain.

Subunit : Interacts with STX1A and STX1B via its v-SNARE homology domain. Part of a complex that contains STX1, STXBP5, SNAP25 and SYT1. Part of a complex that contains STXBP5, STX4A and SNAP23 (By similarity).

Subcellular Location : Cytoplasm (By similarity). Cell membrane; Peripheral membrane protein (By similarity). Cytoplasmic vesicle membrane; Peripheral membrane protein (By similarity). Cytoplasmic vesicle, secretory vesicle, synaptic vesicle (By similarity). Cell junction, synapse (By similarity). Note=Cytoplasmic, and associated with vesicular membranes and the plasma membrane. Detected at synapses and on synaptic vesicles (By similarity).

Similarity : Belongs to the WD repeat L(2)GL family.

Contains 1 v-SNARE coiled-coil homology domain.

Contains 14 WD repeats.

Database links : UniProtKB/Swiss-Prot: Q5T5C0.1

抗体的鉴定：

1）突触融合蛋白结合蛋白5抗体规格 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。

Anti-PSCA /FITC 荧光素标记抗干细胞抗原抗体IgGMulti-class antibodies规格： 0.2ml

Rabbit Anti-pig IgG Whole serum 兔抗猪IgG抗血清Multi-class antibodies规格： 1ml

生长抑制因子基因1抗体 anti-ING1/p33 0.2ml

Rabbit anti-Bovine IgM whole serum 兔抗IgM抗血清 1ml

FAN1 英文名称： 范可尼综合征相关蛋白FAN1抗体 0.2ml

Rhesus antibody Rh phospho-PRKD1(Tyr463) 磷酸化蛋白激酶C mu型抗体 规格 0.1ml

Rabbit Anti-pig IgG Whole serum 兔抗猪IgG抗血清Multi-class antibodies规格： 1ml

BCRP/ABCG2(breast cancer resistance protein) 癌耐药相关蛋白(抗原)Multi-class antibodies规格： 0.5mg

Anti-c-Abl 非受体酪氨酸激酶c-Abl抗体Multi-class antibodies规格： 0.1ml

Rhesus antibody Rh phospho-CHEK1(Ser317) 磷酸化细胞周期检测点激酶1抗体 规格 0.1ml

兔抗Biotin (生物素)血清 0.5ml 国产

WDR16 英文名称： WD重复蛋白16抗体 0.2ml

Phospho-Dab1 (Ser491) 英文名称： 磷酸化Disabled 1抗体 0.1ml

Anti-c-Abl 非受体酪氨酸激酶c-Abl抗体Multi-class antibodies规格： 0.1ml

Anti-CD167a(DDR1)/HRP 辣根过氧化物酶标记CD167a抗体IgGMulti-class antibodies规格： 0.2ml

ECE1(Endothelin Converting Enzyme 1) 内皮素转化酶1抗原Multi-class antibodies规格： 0.5mg

ADP核糖基化因子相互作用蛋白1抗体 Anti-Arfaptin 1 0.1ml

ZNF146 英文名称： 锌指蛋白146抗体 0.2ml

DHFR 英文名称： 二氢叶酸还原酶抗体 0.1ml

Rhesus antibody Rh phospho-eIF4EBP1/4EBP1(Ser65/Thr70) 磷酸化eIF4E结合蛋白抗体 规格 0.1ml

ECE1(Endothelin Converting Enzyme 1) 内皮素转化酶1抗原Multi-class antibodies规格： 0.5mg

人橙(AO)ELISA 试剂盒 96T/48T 试剂盒 组装/原装

兔子碱性成纤维细胞生长因子(bFGF)免疫试剂盒 Rabbit basic fibroblast growth factor,bFGF ELISA Kit

神经穿透素1(NPTX-1)ELISA试剂盒 ，英文名： NPTX-1 ELISA Kit

Porcineierleukin2,IL-2ELISA试剂盒猪白介素2(IL-2)ELISA试剂盒规格：96T/48T

HumanAlanineAminoansferase,ALT/GPTELISA试剂盒人氨酸转氨酶/谷转氨酶(ALT/GPT)ELISA试剂盒规格：96T/48T

Humanapelin13,AP13ELISAKit人apelin13(AP13)ELISA试剂盒规格：96T/48T

大鼠水通道蛋白2(AQP-2) ELISA 试剂盒 96T/48T 试剂盒 组装/原装

人铁调素25(Hepc25)免疫试剂盒 Human Hepcidin 25,Hepc25 ELISA Kit

几丁质酶测试盒 可见分光光度法 50管/24样

RatsolubleToll-likereceptor2,sTLR-2ELISA试剂盒大鼠可溶性Toll样受体2(sTLR2)ELISA试剂盒规格：96T/48T

HumanKallikrein10,KLK10ELISA试剂盒人激肽释放酶10(KLK10)ELISA试剂盒规格：96T/48T

HumanNervegrowthfactor,NGFELISAKit人的神经生长因子(NGF)ELISA试剂盒规格：96T/48T

突触融合蛋白结合蛋白5抗体规格 大鼠维生素C(VC)ELISA试剂盒 ，英文名： VC ELISA Kit

人颗粒酶A(Gzms-A)ELISA检测试剂盒HumangranzymesA,Gzms-AELISAKit 96T/48T

仓鼠坏死因子α(TNF-α)免疫试剂盒 Hamster Tumor necrosis factor α,TNF-α ELISA kit

CLIAKitforRBC(Ratai-redcellaibody)ELISAKit大鼠抗红细胞抗体规格：48T/96T

石蜡切片特恩布尔(TURNBULL-DAB)非血红素铁(二价)强化染色试剂盒50次

ELISAKitoligomeric人寡聚蛋白规格：48T/96T