产品订购信息：
英文名称  Anti-TSHZ3/ZNF537 

中文名称   锌指蛋白537抗体规格 

别    名  teashirt homolog 3; Zinc finger protein 537; TSHZ3; TSH3; ZNF537.

浓    度   1mg/1ml

规 格  0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Chicken, Pig, Cow, Horse, Rabbit 

产品类型   一抗  

研究领域     细胞生物 发育生物学 转录调节因子 表观遗传学

蛋白分子量  predicted molecular weight: 119kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human TSHZ3/ZNF537 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

锌指蛋白537抗体规格 产品应用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 The homeobox DNA-binding domain is a 60 amino acid motif that is conserved among many species and functions to bind DNA via a helix-turn-helix structure, thereby playing a role in transcriptional regulation and the control of gene expression. TSHZ3 (teashirt zinc finger homeobox 3), also known as KIAA1474, TSH3 or ZNF537, is a 1,081 amino acid protein that contains one homeobox DNA-binding domain and five C2H2-type zinc fingers. Localized to the nucleus, TSHZ3 functions as a transcriptional regulator that is involved in developmental processes throughout the body. The gene encoding TSHZ3 maps to human chromosome 19, which consists of over 63 million bases, houses approximately 1,400 genes and is recognized for having the greatest gene density of the human chromosomes.

Function : Transcriptional regulator involved in developmental processes. Function in association with APBB1, SET and HDAC factors as a transcriptional repressor, that inhibits the expression of CASP4. TSHZ3-mediated transcription repression involves the recruitment of histone deacetylases HDAC1 and HDAC2. Associates with chromatin in a region surrounding the CASP4 transcriptional start site(s). Regulates the development of neurons involved in both respiratory rhythm and airflow control. Promotes maintenance of nucleus ambiguus (nA) motoneurons, which govern upper airway function, and establishes a respiratory rhythm generator (RRG) activity compatible with survival at birth. Involved in the differentiation of the proximal uretic smooth muscle cells during developmental processes. Involved in the up-regulation of myocardin, that directs the expression of smooth muscle cells in the proximal ureter.

Subunit : Interacts (via homeobox domain) with APBB1 (via PID domain 1). Interacts (via N-terminus) with HDAC1 and HDAC2; the interaction is direct. Found in a trimeric complex with APBB1 and HDAC1; the interaction between HDAC1 and APBB1 is mediated by TSHZ3.

Subcellular Location : Nucleus. Cell projection, growth cone. Note=Colocalizes with APBB1 in axonal grwoth cone. Colocalizes with APBB1 in the nucleus.

Tissue Specificity : Expressed in brain; strongly reduced in post-mortem elderly subjects with Alzheimer disease.

Similarity : Belongs to the teashirt C2H2-type zinc-finger protein family.

Contains 5 C2H2-type zinc fingers.

Contains 1 homeobox DNA-binding domain.

Anti-SIAH1/FITC 荧光素标记泛素连接酶Siah1抗体IgGMulti-class antibodies规格： 0.2ml

Rabbit Anti-rat IgM 兔抗大鼠IgM (亲和层析纯化)Multi-class antibodies规格： 1mg

三磷酸鸟苷环水解酶抗体 Anti-GTP-CH-1 0.2ml

Dog IgM/Cy3 Cy3标记的兔抗犬IgM抗体 0.1ml

FAM59B 英文名称： FAM59B蛋白抗体 0.2ml

Rhesus antibody Rh phospho-RUNX2(Ser451) 磷酸化成骨特异性转录因子抗体 规格 0.1ml

Rabbit Anti-rat IgM 兔抗大鼠IgM (亲和层析纯化)Multi-class antibodies规格： 1mg

phospho-HP1/heterochromatin protein 1 (pTyr24)(fruit fly) 异染色质蛋白1(磷酸化多肽)Multi-class antibodies规格： 0.5mg

Anti-ABCA1 腺苷三磷酸结合盒转运体A1抗体Multi-class antibodies规格： 0.1ml

Rhesus antibody Rh phospho-c-Jun(Ser63) 磷酸化原癌基因c-Jun抗体 规格 0.1ml

兔抗狗IgG血清 0.5ml 国产

WISP39 英文名称： p21调控蛋白WISP39抗体 0.2ml

DRD4 英文名称： 多巴胺受体D4抗体 0.1ml

Anti-ABCA1 腺苷三磷酸结合盒转运体A1抗体Multi-class antibodies规格： 0.1ml

Anti-XIAP/FITC 荧光素标记X-连锁凋亡蛋白/性连锁凋亡抑制蛋白抗体IgGMulti-class antibodies规格： 0.2ml

CX36 (Connexin-36) 间隙连接蛋白36抗原Multi-class antibodies规格： 0.5mg

肝再生增强因子抗体 Anti-ALR 0.1ml

ZNF717 英文名称： 锌指蛋白717抗体 0.2ml

DBX1 英文名称： 脑发育同源蛋白1抗体 0.2ml

Rhesus antibody Rh Phospho-FAK(Tyr407) 磷酸化粘着斑激酶抗体 规格 0.1ml

CX36 (Connexin-36) 间隙连接蛋白36抗原Multi-class antibodies规格： 0.5mg

人白C 抗原(DR15)ELISA试剂盒 96T/48T 试剂盒 组装/原装

兔子可溶性CD40配体(sCD40L)免疫试剂盒 Rabbit soluble cluster of differeiation 40 ligand,sCD40L ELISA Kit

神经特异性烯醇化酶(NSE)ELISA试剂盒 ，英文名： NSE ELISA Kit

Porcineiercellularadhesionmolecule2,ICAM-2ELISA试剂盒猪细胞间粘附分子2(ICAM-2/CD102)ELISA试剂盒规格：96T/48T

Humanadenylylcyclase1,AC-1ELISA试剂盒人腺苷酸环化酶1(AC-1)ELISA试剂盒规格：96T/48T

HumanAi-ThrombinⅢ,AT-ⅢELISAKit人抗凝血酶Ⅲ抗体(AT-Ⅲ)ELISA试剂盒规格：96T/48T

大鼠松弛肽/松弛素(RLN)ELISA试剂盒 ，英文名： RLN ELISA Kit

人组织蛋白去乙酰化酶(HD)ELISA检测试剂盒HumanHistoneDeacetylase,HDELISAKit 96T/48T

类人猿降钙素(CT)免疫试剂盒 Simian Calcitonin,CT ELISA Kit

CLIAKitforP-PKCELISAKit大鼠磷酸化蛋白激酶C规格：48T/96T

石蜡切片组织FSH-BETA蛋白表达荧光显微镜检测试剂盒10/20次

ELISAKitSIgA人分泌型免疫球蛋白A规格：48T/96T

锌指蛋白537抗体规格 大鼠尾加压素II(Urotensin II) ELISA试剂盒 96T/48T ELISA试剂盒 组装/原装

人维生素H(VH)免疫试剂盒 Human Vitamin H,VH ELISA Kit

脂肪酸合成酶(FAS)测试盒 紫外分光光度法 25管/24样

RatProgesteronereceptor,PROGRELISA试剂盒小鼠孕同受体(PROGR)ELISA试剂盒规格：96T/48T

Humaniercellularadhesionmolecule2,ICAM-2ELISA试剂盒人细胞间粘附分子2(ICAM-2/CD102)ELISA试剂盒规格：96T/48T

humanmitogenactivatedproteinkinasekinase6,MKK6ELISAKit人原活化蛋白激酶激酶6(MKK6)ELISA试剂盒规格：96T/48T

抗体的生物素化标记实验要点：

1. 锌指蛋白537抗体规格 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及苯酚基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

抗体的鉴定：

1）锌指蛋白537抗体规格 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。