抗体的生物素化标记实验要点：

1. 端粒调控因子抗体费用 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及苯酚基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

产品订购信息：
英文名称  Anti-Tankyrase 

中文名称   端粒调控因子抗体费用 

别    名  PARP 5a; PARP5A; PARPL; pART5; Poly [ADP-ribose] polymerase 5A; TANK 1; TANK1; Tankyrase 1; Tankyrase I; Tankyrase TRF1 interacting ankyrin related ADP ribose polymerase; Tankyrase-1; Tankyrase1; TankyraseI; TIN 1; TIN1; TINF 1; TINF1; TNKS 1; TNKS; TNKS-1; TNKS1; TNKS1_HUMAN; TRF1 interacting ankyrin related ADP ribose polymerase; TRF1-interacting ankyrin-related ADP-ribose polymerase.

浓    度   1mg/1ml

规 格  0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Chicken, Dog, Cow 

产品类型   一抗  

研究领域    细胞生物 免疫学 表观遗传学

蛋白分子量  predicted molecular weight: 146kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human Tankyrase 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

端粒调控因子抗体费用 产品应用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 Tankyrase-1 and the closely related homolog Tankyrase-2 arepoly(ADP-ribose) polymerases (PARPs) that co-localize and use an ankyrin-repeat domain to bind diverse proteins, including TRF-1 (telomere-repeat-binding factor 1), IRAP (insulin-responsive aminopeptidase) and TAB182. Tankyrase-1 (also known as TNKS and TNKS1) and Tankyrase-2 (also known as TNKS2, TNKL and TANK2) interact with the same set of proteins and probably mediate overlapping functions, both at telomeres and in vesicular compartments. Overexpression of Tankyrase-1 in the nucleus promotes telomere elongation, suggesting that Tankyrase 1 may regulate access of telomerase to the telomeric complex. Overexpression of Tankyrase-2 in the nucleus releases endogenous TRF1 from telomeres, establishing Tankyrase-2 as a PARP with itself and TRF1 as acceptors of ADP-ribosylation, and suggesting the possibility of a role for Tankyrase-2 at telomeres. The ankyrin (ANK) domain of Tankyrase-2 comprises five subdomains that provide redundant binding sites for IRAP. Tankyrase-2 lacks the N-terminal Histidine/Proline/Serine-rich region of Tankyrase-1, but contains a corresponding ankyrin repeat region, sterile ?motif module and poly(ADP-ribose) polymerase homology domain. The gene encoding Tankyrase-2 is widely expressed, with mRNA transcripts particularly abundant in skeletal muscle and placenta.

Subunit : Oligomerizes and associates with TNKS2. Interacts with the cytoplasmic domain of LNPEP/Otase in SLC2A4/GLUT4-vesicles. Binds to the N-terminus of telomeric TERF1 via the ANK repeats. Found in a complex with POT1; TERF1 and TINF2. Interacts with AXIN1, AXIN2, BLZF1 and CASC3.

Subcellular Location : Cytoplasm. Golgi apparatus membrane. Chromosome > centromere. Nucleus > nuclear pore complex. Chromosome > telomere. Associated with the Golgi and with juxtanuclear SLC2A4/GLUT4-vesicles. A minor proportion is also found at nuclear pore complexes and around the pericentriolar matrix of mitotic centromeres. During interphase, a small fraction of TNKS is found in the nucleus, associated with TERF1.

Tissue Specificity : Ubiquitous; highest levels in testis.

Similarity : Contains 15 ANK repeats.

Contains 1 PARP catalytic domain.

Contains 1 SAM (sterile alpha motif) domain.

抗体的鉴定：

1）端粒调控因子抗体费用 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。

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血红素氧合酶-1/热休克蛋白-32抗体 Anti-HO-1/HSP32 0.1ml

Goat Anti-rat IgG 羊抗大鼠IgG 1mg

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Rat IgM (亲和纯化) 大鼠IgMMulti-class antibodies规格： 1mg

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Anti-phospho-Acinus(Ser1180) 磷酸化腺泡Acinus蛋白抗体Multi-class antibodies规格： 0.1ml

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IgG 干粉 10mg/50mg/100mg 国产

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Socs 3 (suppressor of cytokine signaling 3) 细胞因子信号传导抑制蛋白3抗原Multi-class antibodies规格： 0.5mg

Anti-BMP8 骨形态发生蛋白8抗体Multi-class antibodies规格： 0.2ml

Rhesus antibody Rh Persephin PSP抗体 规格 0.1ml

COX2 环氧化物酶二 浓缩液 0.1ml 进口分装

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C3Orf34 英文名称： 3号染色体开放阅读框34抗体 0.2ml

Anti-BMP8 骨形态发生蛋白8抗体Multi-class antibodies规格： 0.2ml

人白介素15(IL-15)ELISA 试剂盒 96T/48T 试剂盒 组装/原装

兔子硫氧化还原蛋白(x)免疫试剂盒 Rabbit Thioredoxin,x ELISA Kit

神经型合成酶(nNOS)ELISA试剂盒 ，英文名： nNOS ELISA Kit

PorcineHydrocoisone,HYDELISA试剂盒猪(HYD)ELISA试剂盒规格：96T/48T

HumanAcylationStimulatingProtein,ASPELISA试剂盒人酰化刺激蛋白(ASP)ELISA试剂盒规格：96T/48T

Humanai-Sa-aibodyELISAKit人抗Sa抗体(ai-Sa-Ab)ELISA试剂盒规格：96T/48T

大鼠酸脱氢酶E1(PDH E1) ELISA 试剂盒 96T/48T 试剂盒 组装/原装

人螺旋体(TP)抗体(IgM)免疫试剂盒 Human eponema pallidun(TP)aibody(IgM)ELISA kit

MousevonWillebrandFactor,vWFELISAKit小鼠性因子/瑞斯托霉素辅因子(vWF)ELISA试剂盒规格：96T/48T

石蜡切片组织CDK4蛋白表达NBT显色光学显微镜检测试剂盒10/20次

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humahyroidstimulatingimmunoglobulin,TSIELISAKit人甲状腺刺激免疫球蛋白(TSI)ELISA试剂盒规格：96T/48T

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人尾加压素Ⅱ 免疫试剂盒 Human Urotensin Ⅱ ELISA kit

游离脂肪酸含量测试盒 可见分光光度法 50管/48样

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HumaniactParathormone,i-PTHELISA试剂盒人全段甲状旁腺素(i-PTH)ELISA试剂盒规格：96T/48T

Humanmicroansferrinuria,MTFELISAKit人微量转铁蛋白(MTF)ELISA试剂盒规格：96T/48T