产品订购信息：
英文名称  Anti-SynCAM/TSLC1 

中文名称   细胞粘附分子1抗体说明书 

别    名  CADM1; BL2; Cadm1; CADM1_HUMAN; Cell adhesion molecule 1; DKFZp686F1789; IGSF4; IGSF4A; Immunoglobulin Superfamily Member 4; MGC149785; MGC51880; NECL-2; NECL2; Nectin like 2; Nectin-like protein 2; RA175; SgIGSF; Spermatogenic immunoglobulin superfamily; ST17; sTSLC 1; Synaptic cell Adhesion Molecule; SynCAM; SynCAM1; TSLC-1; TSLC1; Tumor Suppressor in Lung Cancer 1.

浓    度   1mg/1ml

规 格  0.1ml/100μg 0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Chicken, Dog 

产品类型   一抗  

研究领域     免疫学 信号转导

蛋白分子量  predicted molecular weight: 44kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human TSLC1 C-terminus 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

细胞粘附分子1抗体说明书 产品应用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 Mediates homophilic cell-cell adhesion in a Ca(2+)-independent manner. Also mediates heterophilic cell-cell adhesion with CADM3 and PVRL3 in a Ca(2+)-independent manner. Acts as a tumor suppressor in non-small-cell lung cancer (NSCLC) cells. Interaction with CRTAM promotes natural killer (NK) cell cytotoxicity and interferon-gamma (IFN-gamma) secretion by CD8+ cells in vitro as well as NK cell-mediated rejection of tumors expressing CADM3 in vivo. May contribute to the less invasive phenotypes of lepidic growth tumor cells. In mast cells, may mediate attachment to and promote communication with nerves. CADM1, together with MITF, is essential for development and survival of mast cells in vivo. May act as a synaptic cell adhesion molecule that drives synapse assembly. May be involved in neuronal migration, axon growth, pathfinding, and fasciculation on the axons of differentiating neurons. May play diverse roles in the spermatogenesis including in the adhesion of spermatocytes and spermatids to Sertoli cells and for their normal differentiation into mature spermatozoa.

Function : Mediates homophilic cell-cell adhesion in a Ca(2+)-independent manner. Also mediates heterophilic cell-cell adhesion with CADM3 and PVRL3 in a Ca(2+)-independent manner. Acts as a tumor suppressor in non-small-cell lung cancer (NSCLC) cells. Interaction with CRTAM promotes natural killer (NK) cell cytotoxicity and interferon-gamma (IFN-gamma) secretion by CD8+ cells in vitro as well as NK cell-mediated rejection of tumors expressing CADM3 in vivo. May contribute to the less invasive phenotypes of lepidic growth tumor cells. In mast cells, may mediate attachment to and promote communication with nerves. CADM1, together with MITF, is essential for development and survival of mast cells in vivo. May act as a synaptic cell adhesion molecule that drives synapse assembly. May be involved in neuronal migration, axon growth, pathfinding, and fasciculation on the axons of differentiating neurons. May play diverse roles in the spermatogenesis including in the adhesion of spermatocytes and spermatids to Sertoli cells and for their normal differentiation into mature spermatozoa.

Subunit : Homodimer. Interacts with CRTAM and EPB41L3/DAL1. The interaction with EPB41L3/DAL1 may act to anchor CADM1 to the actin cytoskeleton. Interacts via its C-terminus with the PDZ domain of MPP3 and the PDZ domain of MPP6.

Subcellular Location : Cell membrane; Single-pass type I membrane protein. Note=Associates with perinuclear and plasma membranes in vivo. Localized to the basolateral plasma membrane of epithelial cells in gall bladder.

Similarity : Belongs to the nectin family.

Contains 2 Ig-like C2-type (immunoglobulin-like) domains.

Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Database links : UniProtKB/Swiss-Prot: Q9BY67.2

Entrez Gene: 23705 Human

Entrez Gene: 54725 Mouse

Entrez Gene: 363058 Rat

Omim: 605686 Human

SwissProt: Q9BY67 Human

SwissProt: Q8R5M8 Mouse

Unigene: 370510 Human

Unigene: 234832 Mouse

TSLC1属于新的基因免疫球蛋白超家族, TSLC1蛋白质参与细胞间黏附、细胞运动、信号转导及. TSLC1在多种中表达异常,它对的影响主要表现为特性及基因表达,可抑制上皮间质转化.

TSLC1蛋白在控制生物侵袭性方面有着重要作用，也可能作为预测患者预后的重要生物标记，但是TSLC1的抑制分子机制还有待进一步研究。

Anti-JNK1/2/3/FITC 荧光素标记抗氨基末端激酶1/2/3抗体 IgGMulti-class antibodies规格： 0.2ml

Recomb Protein A/Gold 金标记重组蛋白A(10nm/15nm)Multi-class antibodies规格： 0.5ml

叉头蛋白3-T细胞转录因子 Anti-FoxP3 0.1ml

Goat Anti-rabbit IgG/PE PE标记的羊抗兔IgG 0.1ml

FMO5 英文名称： 二甲基苯胺单加氧酶5抗体 0.2ml

Rhesus antibody Rh phospho-STAT3 (Ser727) 磷酸化信号转导和转录激活因子3抗体 规格 0.1ml

Recomb Protein A/Gold 金标记重组蛋白A(10nm/15nm)Multi-class antibodies规格： 0.5ml

IL-4(Interleukin-4) 白介素4抗原Multi-class antibodies规格： 0.5mg

Anti-ADAMTS1 整合素样金属蛋白酶与凝血酶1型抗体Multi-class antibodies规格： 0.1ml

Rhesus antibody Rh phospho-Cdc6 (Ser54) 磷酸化细胞分裂周期蛋白6抗体 规格 0.1ml

免疫组化/原位杂交专用波片(双面) 50片 真空车间清洗硅化处理,表面无污渍

Wnt8A 英文名称： 信号通路WNT8A抗体 0.2ml

DUOXA2 英文名称： 双氧化酶激活因子2抗体 0.2ml

Anti-ADAMTS1 整合素样金属蛋白酶与凝血酶1型抗体Multi-class antibodies规格： 0.1ml

OJ/IleRS(Human anti-OJ-antibody) ELISA Kit 人OJ抗体/抗异亮氨酰tRNA合成酶抗体Multi-class antibodies规格： 48T

Anti-Phospho-PLC gamma 2 (Tyr1217) 磷酸化磷酯酶Cγ2抗体Multi-class antibodies规格： 0.1ml

Rhesus antibody Rh HHV8/ORF50 人类病毒8抗体 规格 0.2ml

ENA-78/CXCL5 ELISA Kit 大鼠上皮中性粒细胞活化肽78 96T

Classical swine fever virus 英文名称： 抗体 0.2ml

C20orf26 英文名称： 20号染色体开放阅读框26抗体 0.2ml

Anti-Phospho-PLC gamma 2 (Tyr1217) 磷酸化磷酯酶Cγ2抗体Multi-class antibodies规格： 0.1ml

人白介素1可溶性受体Ⅱ(IL-1sRⅡ)ELISA 试剂盒 96T/48T 试剂盒 组装/原装

兔子凝血因子Ⅱ(FⅡ)免疫试剂盒 Rabbit coagulation factor Ⅱ,FⅡ ELISA Kit

肾上腺髓质素(ADM)ELISA试剂盒 ，英文名： ADM ELISA Kit

Porcinegrowthhormonereleasingpeptide-Ghrelin,GHRP-GhrelinELISA试剂盒猪生长激素释放肽ghrelin(GHRP-Ghrelin)ELISA试剂盒规格：96T/48T

Humanacidicfibroblastgrowthfactor1,aFGF-1ELISA试剂盒人酸性成纤维细胞生长因子1(aFGF-1)ELISA试剂盒规格：96T/48T

Humanai-Qfeveraibody,ai-Q-AbELISAKit人抗Q热抗体(ai-Q-Ab)ELISA试剂盒规格：96T/48T

大鼠酸性磷酸酶(ACP)ELISA试剂盒 ，英文名： ACP ELISA Kit

人热休克蛋白70(HSP-70)ELISA检测试剂盒HumanHeatShockProtein70,HSP-70ELISAKit 96T/48T

鹿γ干扰素(IFNG)免疫试剂盒 Deer ierferon gamma,IFNG ELISA kit

CLIAKitforPR3Ab(HumanProteinase3Aibody)ELISAKit人蛋白酶3抗体规格：48T/96T

石蜡切片组织DAPKINASE蛋白表达荧光显微镜检测试剂盒10/20次

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细胞粘附分子1抗体说明书 大鼠吻素受体(KISS1R)ELISA试剂盒 ，英文名： KISS1R ELISA Kit

Mouse dihydrotestosterone (DHT) ELISA Kit 小鼠双氢睾酮(DHT)ELISA试剂盒

MouseSmad7ELISAKit 小鼠信号转导分子7(Smad7)ELISA试剂盒 96T/48T 进口分装

CLIAKitforHSL(Humanhormonesensitivelipase)ELISAKit人激素敏感性脂肪酶规格：48T/96T

细胞NADPH细胞色素C还原酶(NADPHCytochromeCReductase)活性比色法定量检测试剂盒20次

ELISAKitOrexinA大鼠阿立新A规格：48T/96T

抗体的生物素化标记实验要点：

1. 细胞粘附分子1抗体说明书 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及苯酚基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

抗体的鉴定：

1）细胞粘附分子1抗体说明书 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。