中文名称  促甲状腺素受体抗体（N端）规格 

英文名称  Anti-TSHR (NT)

别    名 hTSHR I; hTSHRI; LGR 3; LGR3; MGC75129; Thyroid adenoma hyperfunctioning; Thyroid carcinoma with thyrotoxicosis; Thyroid Stimulating Hormone Receptor; Thyrotropin Receptor; Thyrotropin receptor I; TSH R; TSHR; TSHR_HUMAN.

产品属性：

促甲状腺素受体抗体（N端）规格 浓    度  1mg/1ml

规 格  0.1ml/100μg 0.2ml/200μg

抗体来源  Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Dog, Cow, Sheep

产品类型   一抗  

研究领域    免疫学 神经生物学 信号转导 生长因子和激素 细胞膜受体

蛋白分子量  predicted molecular weight: 86kDa 

性    状   Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from human TSHR N-terminus

亚    型  IgG

纯化方法   affinity purified by Protein A

储 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

促甲状腺素受体抗体（N端）规格 产品应用   WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 The glycoprotein hormone receptor family consists of the luteinizing hormone receptor, the follicle-stimulating hormone receptor, and the thyroid stimulating hormone(TSH) receptor. TSH, which is released from the pituitary gland, binds to the TSH receptor on thyroid cells to control size and function of the thyroid gland (De Felice et al. 2004). The TSH receptor signals through Gs to elevate intracellular cAMP in the thyroid gland, which regulates iodide uptake, and transcription of thyroglobulin (Tg), thyroid peroxidase (TPO), and sodium-iodide symporter. The TSH receptor also signals Gq and phospholipase C to regulat iodide efflux, H2O2 production, and thyroglobulin iodination. Autoimmunity to the TSH receptor causes hyperthyroidism (Graves disease) or hypothyroidism (Hashimoto thyroiditis) when the autoantibodies function as agonists or antagonists, respectively, at the TSH receptor (Rapoport and McLachlan, 2001; Davies et al., 2002). Millipore’s cloned human TSH receptor-expressing cell line is made in the Chem-10 host, which supports high levels of recombinant TSH receptor expression on the cell surface and contains high levels of the promiscuous G protein to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between TSH and its ligands.

Function : Receptor for thyrothropin. Plays a central role in controlling thyroid cell metabolism. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. Also acts as a receptor for thyrostimulin (GPA2+GPB5).

Subunit : Interacts (via the PDZ-binding motif) with SCRIB; regulates TSHR trafficking and function.

Subcellular Location : Cell membrane; Multi-pass membrane protein.

Tissue Specificity : Expressed in the thyroid.

DISEASE : Note=Defects in TSHR are found in patients affected by hyperthyroidism with different etiologies. Somatic, constitutively activating TSHR mutations and/or constitutively activating G(s)alpha mutations have been identified in toxic thyroid nodules (TTNs) that are the predominant cause of hyperthyroidism in iodine deficient areas. These mutations lead to TSH independent activation of the cAMP cascade resulting in thyroid growth and hormone production. TSHR mutations are found in autonomously functioning thyroid nodules (AFTN), toxic multinodular goiter (TMNG) and hyperfunctioning thyroid adenomas (HTA). TMNG encompasses a spectrum of different clinical entities, ranging from a single hyperfunctioning nodule within an enlarged thyroid, to multiple hyperfunctioning areas scattered throughout the gland. HTA are discrete encapsulated neoplasms characterized by TSH-independent autonomous growth, hypersecretion of thyroid hormones, and TSH suppression. Defects in TSHR are also a cause of thyroid neoplasms (papillary and follicular cancers).

Similarity : Belongs to the G-protein coupled receptor 1 family. FSH/LSH/TSH subfamily.

Contains 7 LRR (leucine-rich) repeats.

Database links : UniProtKB/Swiss-Prot: P16473.2

TSHR是垂体前叶嗜碱细胞分泌的一种糖蛋白，直接作用于甲状腺，并能影响其结构功能。该抗体主要用于垂体功能性分类的研究。

实验流程：

全、新、优、品、好四大特点：

促甲状腺素受体抗体（N端）规格 全：公司提供上万种产品，涵盖了生物试剂，elisa试剂盒，标准品，培养基，原装耗材，抗体、培养基、ATCC细胞等，基本上各种科研所需产品在我司都能找到。

新：产品更新速度较快，基本上每周都有新产品出现。

优：产品质量好，投诉比较少。

好：我公司具有优质的技术团队，产品一旦售出，实验过程中遇到困难可提供在线技术咨询。使您使用产品时没有任何的后顾之忧。

技术外包服务:

★促甲状腺素受体抗体（N端）规格 分子生物学：质粒抽提、PCR、Q-PCR、RT-PCR、分子生物学：基因合成、引物合成、基因测序、载体构建等

★蛋白工程：原核、哺乳动物蛋白表达系统等

★病毒包装：腺病毒、慢病毒等

★抗体工程：磁珠分选、病理染色、WB、ELISA、IP、IF、IHC、FACS、Confocal等等

★细胞工程：细胞表型分析(凋亡、增殖、周期、迁移、侵袭、修复、克隆形成)、细胞培养、细胞膜制备、稳定细胞株构建、细胞RNAi技术等等。

正在热销的相关产品：

Anti-PTP-1B/FITC 荧光素标记PTP-1B蛋白抗体IgGMulti-class antibodies规格： 0.2ml

Recomb Protein G/FITC 荧光素(FITC)标记重组蛋白-GMulti-class antibodies规格： 0.5ml

粘附分子整合素β1抗体 Anti-ITG-β1/CD29 0.1ml

SOX10 英文名称： 转录因子SOX10抗体 0.2ml

FRAS1 英文名称： 细胞外基质蛋白FRAS1抗体 0.2ml

Rhesus antibody Rh phospho-PPM1A(Ser375) 磷酸化蛋酸酶2C亚型α抗体 规格 0.1ml

Recomb Protein G/FITC 荧光素(FITC)标记重组蛋白-GMulti-class antibodies规格： 0.5ml

IFABP(Intestinal Fatty acid binding protein) 小肠型脂肪酸结合蛋白抗原Multi-class antibodies规格： 0.5mg

Anti-ABP 雄激素结合蛋白抗体Multi-class antibodies规格： 0.2ml

Rhesus antibody Rh Phospho-CENP-A (Ser7) 磷酸化着丝粒蛋白A 规格 0.1ml

兔抗人IgG- FC 0.5ml 国产

WNT9A 英文名称： 信号通路Wnt9a抗体 0.1ml

DAPK1 英文名称： 凋亡相关蛋白激酶1抗体 0.1ml

Anti-ABP 雄激素结合蛋白抗体Multi-class antibodies规格： 0.2ml

Anti-Slc4a7/Nbcn1 /FITC 荧光素标记碳酸氢协同转运蛋白4-A7抗体IgGMulti-class antibodies规格： 0.2ml

AHA3 peptide 植物蛋白AHA3抗原(拟南芥)Multi-class antibodies规格： 0.5mg

NADPH氧化酶4抗体 Anti-Nox-4/NADH 0.1ml

2,4-D 英文名称： 2，4-二氯苯氧乙酸(除草剂)抗体 0.2ml

CHKL 英文名称： 乙醇胺激酶β抗体 0.1ml

Rhesus antibody Rh phospho-P53(Ser392) 磷酸化抑制基因P53抗体 规格 0.1ml

AHA3 peptide 植物蛋白AHA3抗原(拟南芥)Multi-class antibodies规格： 0.5mg

人白介素2(IL-2)ELISA 试剂盒 96T/48T 试剂盒 组装/原装

兔子皮质酮(CO)免疫试剂盒 Rabbit Coicosterone,CO ELISA Kit

肾素(Renin)ELISA试剂盒 ，英文名： Renin ELISA Kit

Porcinegrowthhormonereleasinghormone,GHRHELISA试剂盒猪促生长激素释放激素(GHRH)ELISA试剂盒规格：96T/48T

HumanAcidPhosphatase,ACPELISA试剂盒人酸性磷酸酶(ACP)ELISA试剂盒规格：96T/48T

HumanAi-proteinase3aibodyIgG,PR3Ab-IgGELISAKit人抗蛋白酶3抗体IgG(PR3Ab-IgG)ELISA试剂盒规格：96T/48T

大鼠酸性磷酸酶1(ACP1)ELISA试剂盒 ，英文名： ACP1 ELISA Kit

Mouse glycogen phosphorylase MM (GP-MM) ELISA Kit 小鼠糖原磷酸化酶同工酶MM(GP-MM)ELISA试剂盒

Ratbradykinin,BKELISAKit 大鼠舒缓激肽(BK)ELISA试剂盒 96T/48T 进口分装

CLIAKitforhiston-H2b(Mousehiston-H2b)ELISAKit小鼠组蛋白H2b规格：48T/96T

细胞ROCK-II激酶活性定量检测试剂盒(A/B/C)20次

ELISAKitColⅣ大鼠Ⅳ型胶原规格：48T/96T

促甲状腺素受体抗体（N端）规格 大鼠无翅型MMTV整合位点家族成员3A(W3A)ELISA试剂盒 ，英文名： W3A ELISA Kit

Mouse eosinophil chemotactic factor (ECF) ELISA Kit 小鼠嗜酸粒细胞趋化因子(ECF)ELISA试剂盒

Mouseplatelet-derivedgrowthfactor-BB,PDGF-BBELISAKit 小鼠血小板衍生生长因子BB(PDGF-BB)ELISA试剂盒 96T/48T 进口分装

CLIAKitforHSPgp96(HumanHeatShockProteinglycoprotein96)ELISAKit人热休克蛋白糖蛋白96规格：48T/96T

细胞MUSK激酶活性定量检测试剂盒(A/B/C)20次

ELISAKitIL-10大鼠白介素10规格：48T/96T