产品订购信息：
英文名称  Anti-TSPAN12/NET-2 

中文名称   四分子交联体12抗体（四旋蛋白）说明书 

别    名  TSPAN 12; NET 2; NET2; Tetraspan NET 2; Tetraspanin 12; Tetraspanin12; TM4SF12; Transmembrane 4 superfamily member 12; TSN12_HUMAN.

浓    度   1mg/1ml

规 格  0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep 

产品类型   一抗  

研究领域    细胞生物 神经生物学 信号转导 通道蛋白 Alzheimer's

蛋白分子量  predicted molecular weight: 35kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human TSPAN12/NET-2 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

四分子交联体12抗体（四旋蛋白）说明书 产品应用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:20-100 ICC= IF=1:50-200

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 TSPAN12 is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. It is a regulator of cell surface receptor signal transduction and plays a central role in retinal vascularization by regulating norrin (NDP) signal transduction. It acts in concert with norrin (NDP) to promote FZD4 multimerization and subsequent activation of FZD4, leading to promote accumulation of beta-catenin (CTNNB1) and stimulate LEF/TCF-mediated transcriptional programs. It also acts as a regulator of membrane proteinases such as ADAM10 and MMP14/MT1-MMP. It activates ADAM10-dependent cleavage activity of amyloid precursor protein (APP) and activates MMP14/MT1-MMP-dependent cleavage activity.

Function : Regulator of cell surface receptor signal transduction. Plays a central role in retinal vascularization by regulating norrin (NDP) signal transduction. Acts in concert with norrin (NDP) to promote FZD4 multimerization and subsequent activation of FZD4, leading to promote accumulation of beta-catenin (CTNNB1) and stimulate LEF/TCF-mediated transcriptional programs. Suprisingly, it only activate the norrin (NDP)-dependent activation of FZD4, while it does not activate the Wnt-dependent activation of FZD4, suggesting the existence of a Wnt-independent signaling that also promote accumulation the beta-catenin (CTNNB1) (By similarity). Acts as a regulator of membrane proteinases such as ADAM10 and MMP14/MT1-MMP. Activates ADAM10-dependent cleavage activity of amyloid precursor protein (APP). Activates MMP14/MT1-MMP-dependent cleavage activity.

Subunit : Component of a complex, at least composed of TSPAN12, FZD4 and norrin (NDP) (By similarity). Interacts (when palmitoylated) with ADAM10. Interacts with MMP14/MT1-MMP.

Subcellular Location : Cell membrane; Multi-pass membrane protein.

Post-translational modifications : Palmitoylated; required for interaction with ADAM10. The precise position of palmitoylated residues is unclear and occurs either on Cys-9, Cys-12 and/or Cys-83.

DISEASE : Defects in TSPAN12 are the cause of vitreoretinopathy exudative type 5 (EVR5) [MIM:613310]. It is a disorder of the retinal vasculature characterized by an abrupt cessation of growth of peripheral capillaries, leading to an avascular peripheral retina. This may lead to compensatory retinal neovascularization, which is thought to be induced by hypoxia from the initial avascular insult. New vessels are prone to leakage and rupture causing exudates and bleeding, followed by scarring, retinal detachment and blindness. Clinical features can be highly variable, even within the same family. Patients with mild forms of the disease are asymptomatic, and their only disease related abnormality is an arc of avascular retina in the extreme temporal periphery. Note=TSPAN12 dominant and recessive mutations have been identified in patients with exudative vitreoretinopathy. Patients with mutations in both alleles of TSPAN12 have severe exudative vitreoretinopathy or retinal dysplasia. These mutations appear to result in a milder phenotype in heterozygous mutation carriers (PubMed:22427576).

Similarity : Belongs to the tetraspanin (TM4SF) family.

Database links : UniProtKB/Swiss-Prot: O95859.1

Anti-TRP2/FITC 荧光素标记酪氨酸酶相关蛋白2抗体IgGMulti-class antibodies规格： 0.2ml

TGF- Beta1 (Ttansforming Growth Factor – Beta1) 转移生长因子–β1(抗原)Multi-class antibodies规格： 0.5mg

内皮细胞粘附分子抗体 Anti-VCAM-1/CD106 0.1ml

SETBP1 英文名称： SET结合蛋白1抗体 0.2ml

ECRG4 英文名称： 相关基因4蛋白抗体 0.1ml

Rhesus antibody Rh phospho-ILK-1(Ser246) 磷酸化整合素连接激酶1抗体 规格 0.1ml

TGF- Beta1 (Ttansforming Growth Factor – Beta1) 转移生长因子–β1(抗原)Multi-class antibodies规格： 0.5mg

FRA2/FOSL2(Fos related/like antigen 2) FOS样抗原2Multi-class antibodies规格： 0.5mg

ALDH7A1 ALDH7A1 兔单抗 (PE) Human

Rhesus antibody Rh phospho-Dnmt1(Tyr969) 磷酸化DNA甲基转移酶1抗体 规格 0.1ml

鼠兔通用WB套装试剂盒 2500cm2 原装

ZDHHC21 英文名称： γ-基A受体相关膜蛋白3 0.2ml

phospho-DAPP1 (Tyr139) 英文名称： 磷酸化B淋巴细胞衔接分子DAPP1抗体 0.1ml

ALDH7A1 ALDH7A1 兔单抗 (PE) Human

Anti-CRF/CRH /FITC 荧光素标记促肾上腺皮质激素释放因子抗体IgGMulti-class antibodies规格： 0.2ml

Anti-κOR/FITC 荧光素标记kappa型受体抗体IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh CCDCNL 卷曲螺旋结构域蛋白NL抗体 规格 0.2ml

UTS2R/GPR14(Urotensin II receptor：G Protein Coupled Receptor 14) G蛋白偶联受体14抗原 0.5mg

C14orf94 英文名称： 14号染色体开放阅读框94抗体 0.2ml

Rhesus antibody Rh SM Myosin (Smooth Muscle) 人心肌肌凝蛋白(平滑肌) 小鼠单抗 规格 0.2ml

Anti-κOR/FITC 荧光素标记kappa型受体抗体IgGMulti-class antibodies规格： 0.2ml

人毒素(PT)ELISA 试剂盒 96T/48T 试剂盒 组装/原装

兔子血小板衍生生长因子AB(PDGF-AB)免疫试剂盒 Rabbit Platelet-Derived Growth Factor AB,PDGF-AB ELISA Kit

瘦素受体(Lepti)ELISA试剂盒 ，英文名： Lepti ELISA Kit

PlaVitaminD,VDELISA试剂盒植物维生素D(VD)ELISA试剂盒规格：96T/48T

GuineaPigplasmin-aiplasmincomplex,PAPELISA试剂盒豚鼠纤溶酶抗纤溶酶复合物(PAP)ELISA试剂盒规格：96T/48T

Humanai-glycoproteinaibody，GPELISAKit人抗糖蛋白抗体(GP)ELISA试剂盒规格：96T/48T

大鼠糖皮质激素受体α(GR-α)ELISA 试剂盒 96T/48T 试剂盒 组装/原装

人脱氧皮质酮(DOC)免疫试剂盒 Human Deoxycoicosterone,DOC ELISA Kit

蔗糖磷酸合成酶(SPS)测试盒 可见分光光度法 50管/48样

Ratsolubleclusterofdiffereiation14,sCD14ELISA试剂盒大鼠可溶性CD14(sCD14)ELISA试剂盒规格：96T/48T

HumanIerleukin5,IL-5ELISA试剂盒人白介素-5(IL-5)ELISA试剂盒规格：96T/48T

humanmyeloiddiffereiationprimaryresponsegene88,MYD88ELISAKit人髓样分化因子初次应答基因88(MYD88)ELISA试剂盒规格：96T/48T

四分子交联体12抗体（四旋蛋白）说明书 大鼠细胞色素P450氧化还原酶(CPR)ELISA试剂盒 ，英文名： CPR ELISA Kit

Mouse neuron specific enolase (NSE) ELISA Kit 小鼠神经特异性烯醇化酶(NSE)ELISA试剂盒

Mouseapoprotein,apo-A1ELISAKit 小鼠载脂蛋白A1(apo-A1)ELISA试剂盒 96T/48T 进口分装

CLIAKitforHSP-90(HumanHeatShockProtein90)ELISAKit人热休克蛋白90规格：48T/96T

细胞JNK2激酶活性定量检测试剂盒(A/B/C)20次

ELISAKitIL-2sRα大鼠白介素2可溶性受体α链规格：48T/96T

抗体的生物素化标记实验要点：

1. 四分子交联体12抗体（四旋蛋白）说明书 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及苯酚基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

抗体的鉴定：

1）四分子交联体12抗体（四旋蛋白）说明书 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。