产品订购信息：
英文名称  Anti-BTRC2/beta TRCP2 

中文名称   BTRC2蛋白抗体品牌 

别    名  beta Transducin Repeat Containing Protein 2; BTRC2; BTRCP2; F-box and WD-40 Domain Protein 1B; F-box Protein FBW1B; FBW1B; FBXW1B; FBW1B_HUMAN.

浓    度   1mg/1ml

规 格  0.1ml/100μg 0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Chicken, Pig, Cow, Horse, Zebrafish 

产品类型   一抗  

研究领域    细胞生物 免疫学 细胞周期蛋白

蛋白分子量  predicted molecular weight: 62kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human BTRC2/beta TRCP2 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

BTRC2蛋白抗体品牌 产品应用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 Beta TRCP2 is a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbws class and, in addition to an F-box, contains multiple WD40 repeats. This gene contains at least 14 exons, and its alternative splicing generates 3 transcript variants diverging at the presence/absence of two alternate exons.

Function : Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes and binds to phosphorylated target proteins. SCF(BTRC) mediates the ubiquitination of CTNNB1 and participates in Wnt signaling. SCF(BTRC) mediates the ubiquitination of NFKBIA, NFKBIB and NFKBIE; the degradation frees the associated NFKB1 to translocate into the nucleus and to activate transcription. Ubiquitination of NFKBIA occurs at 'Lys-21' and 'Lys-22'. SCF(BTRC) mediates the ubiquitination of phosphorylated NFKB1/nuclear factor NF-kappa-B p105 subunit, ATF4, SMAD3, SMAD4, CDC25A, DLG1, FBXO5 and probably NFKB2. SCF(BTRC) mediates the ubiquitination of phosphorylated SNAI1. May be involved in ubiquitination and subsequent proteasomal degradation through a DBB1-CUL4 E3 ubiquitin-protein ligase. Required for activation of NFKB-mediated transcription by IL1B, MAP3K14, MAP3K1, IKBKB and TNF. Required for proteolytic processing of GLI3.

Subunit : Self-associates. Component of the SCF(FBXW11) complex formed of CUL1, SKP1, RBX1 and a FBXW11 dimer. Interacts with BTRC, BST2, PER1, RCAN1 and USP47. Interacts with phosphorylated ubiquitination substrate PER2 (By similarity). Interacts with phosphorylated ubiquitination substrates CTNNB1, NFKBIA, IFNAR1; the interaction requires the phosphorylation of the two serine residues in the substrates' destruction motif D-S-G-X(2,3,4)-S. Interacts with TRIM21.

Subcellular Location : Cytoplasm. Nucleus.

Similarity : Contains 1 F-box domain.

Contains 7 WD repeats.

Database links : UniProtKB/Swiss-Prot: Q9UKB1.1

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EDA 英文名称： 外胚层蛋白1抗体 0.2ml

Rhesus antibody Rh phospho-IKK beta(Ser466) 磷酸化KB抑制蛋白激酶β抗体 规格 0.1ml

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FN(Mouse Fibronectin) ELISA Kit 小鼠纤连蛋白Multi-class antibodies规格： 48T

Anti-CCL23/MPIF-1/FITC 荧光素标记因子1抗体IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh EDNRA 内皮素受体A抗体 规格 0.1ml

Fish Cortisol ELISA Kit 鱼类皮质醇 96T

ZNF 英文名称： 锌指蛋白抗体 0.2ml

phospho-ATF2 (Thr51 + Thr53) 英文名称： 磷酸化活化复制因子2抗体 0.1ml

Anti-CCL23/MPIF-1/FITC 荧光素标记因子1抗体IgGMulti-class antibodies规格： 0.2ml

MAPK1/ERK3 (Mitogen-activated protein kinase 3; P44MAPK) 原活化蛋白激酶3抗原Multi-class antibodies规格： 0.5mg

缓激肽B2受体抗体 Anti-Bdkrb2/B2R 0.1ml

Rhesus antibody Rh phospho-EGFR (Tyr1092) 磷酸化表皮生长因子受体抗体 规格 0.1ml

N2添加剂 (原装) 5ml Gibco

ANKZF1 英文名称： 锌指蛋白744抗体 0.2ml

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缓激肽B2受体抗体 Anti-Bdkrb2/B2R 0.1ml

人鞭毛蛋白(flagellin)ELISA 试剂盒 96T/48T 试剂盒 组装/原装

兔子组织多肽抗原(TPA)免疫试剂盒 Rabbit Tissue Polypeptide Aigen,TPA ELISA Kit

丝酸蛋白酶HA1(HA1)ELISA试剂盒 ，英文名： HA1 ELISA Kit

Nasoniaviipennisvitellogenin,VtgELISA试剂盒蝶蛹金小蜂卵黄蛋白原(vtg)ELISA试剂盒规格：96T/48T

guineapighepatocytegrowthfactor,HGFELISA试剂盒豚鼠肝细胞生长因子(HGF)ELISA试剂盒规格：96T/48T

HumanAi-Compleme1qaibody,C1qELISAKit人抗补体1q抗体(C1q)ELISA试剂盒规格：96T/48T

大鼠糖原磷酸化酶M(PYGM)ELISA试剂盒 ，英文名： PYGM ELISA Kit

Mouse placea growth factor (PLGF) ELISA Kit 小鼠胎盘生长因子(PLGF)ELISA试剂盒

Rat17-Hydroxyprogesterone,17-OHPELISAKit 大鼠17羟孕酮(17-OHP)ELISA试剂盒 96T/48T 进口分装

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细胞PKCδ激酶活性定量检测试剂盒(A/B/C)20次

ELISAKi-proBNP大鼠N端前脑素规格：48T/96T

BTRC2蛋白抗体品牌 大鼠细胞周期素D3(Cyclin-D3)ELISA试剂盒 ，英文名： Cyclin-D3 ELISA Kit

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石蜡切片结缔组织(CONNECTIVETISSUE)马森(MASSON)染色试剂盒50次

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抗体的生物素化标记实验要点：

1. BTRC2蛋白抗体品牌 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及苯酚基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

抗体的鉴定：

1）BTRC2蛋白抗体品牌 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。