产品订购信息：
英文名称  Anti-TWF2/PTK9L 

中文名称   细胞骨架结合蛋白2抗体说明书 

别    名  A6 related protein; A6r; A6RP; hA6RP; MSTP011; Protein tyrosine kinase 9 like; PTK 9L; PTK9L;PTK9L; PTK9L protein tyrosine kinase 9 like; TWF 2; Twinfilin 1 like protein; Twinfilin 2; Twinfilin actin binding protein homolog 2; Twinfilin like protein; Twinfilin2; TWF2_HUMAN.

浓    度   1mg/1ml

规 格  0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Sheep 

产品类型   一抗  

研究领域    神经生物学 结合蛋白 细胞骨架

蛋白分子量  predicted molecular weight: 39kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human TWF2/PTK9L 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

细胞骨架结合蛋白2抗体说明书 产品应用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 Twinfilin-2 is a 349 amino acid protein that localizes to the perinuclear region of the cytoplasm, as well as to the cytoskeleton, and contains two ADF-H domains. Expressed ubiquitously, Twinfilin-2 functions as an Actin-binding protein that is able to both bind and sequester G-Actin and cap the barbed ends of Actin filaments, thereby inhibiting Actin polymerization and playing a role in Actin-related motile and morphological processes. Twinfilin-2 is subject to post-translational phosphorylation by PKC zeta. The gene encoding Twinfilin-2 maps to human chromosome 3, which houses over 1,100 genes, including a chemokine receptor (CKR) gene cluster and a variety of human cancer-related gene loci.

Function : TWF2 (Twinfilin 2) was identified by its interaction with the catalytic domain of protein kinase C zeta. It contains an actin binding site and an ATP binding site and is most closely related to twinfilin (PTK9), a conserved actin monomer binding protein. TWF2 is an actin binding protein involved in motile and morphological processes. It inhibits actin polymerization, likely by sequestering G actin. By capping the barbed ends of filaments, it also regulates motility. It seems to play an important role in clathrin mediated endocytosis and distribution of endocytic organelles.

Subunit : Interacts with G-actin; ADP-actin form and capping protein (CP). May also be able to interact with TWF1 and phosphoinositides, PI(4,5)P2. When bound to PI(4,5)P2, it is down-regulated. Interacts with MYO7A

Subcellular Location : Cytoplasmic, cytoskeleton. Cytoplasmic, perinuclear region. Note: Perinuclear and G actin rich cortical actin structures sublocalization.

Tissue Specificity : Ubiquitously expressed (at protein level).

Post-translational modifications : In vitro, phosphorylated by PRKCZ, CK2 and SRC.

Similarity : Belongs to the actin-binding proteins ADF family. Twinfilin subfamily.

Contains 2 ADF-H domains.

Database links : UniProtKB/Swiss-Prot: Q6IBS0.2

AFP ELISA Kit 大鼠甲种胎儿球蛋白/甲胎蛋白Multi-class antibodies规格： 48T

单抗 单抗Multi-class antibodies规格：

Rhesus antibody Rh FRMD1 FRMD1蛋白抗体 规格 0.2ml

AGEs ELISA Kit 大鼠晚期糖基化终末产物 96T

Neurofascin 英文名称： 神经束蛋白抗体 0.1ml

AHNAK 英文名称： 神经细胞分化相关蛋白AHNAK抗体 0.1ml

单抗 单抗Multi-class antibodies规格：

phospho-FAK(pTyr577) 磷酸化粘着斑激酶抗原Multi-class antibodies规格： 0.5mg

桥连整合因子蛋白抗体 Anti-BIN1 0.1ml

Rhesus antibody Rh Phospho-EEF2 (Thr56) 磷酸化真核翻译延长因子2抗体 规格 0.1ml

品名 规格 备注

ZNF342 英文名称： 锌指蛋白342抗体 0.2ml

phospho-DAP Kinase 1 (Ser 308) 英文名称： 磷酸化凋亡相关蛋白激酶1抗体 0.1ml

桥连整合因子蛋白抗体 Anti-BIN1 0.1ml

Anti-phospho-FOXO4 (phospho S197) /FITC 荧光素标记兔抗人、大、小鼠叉头蛋白4抗体IgGMulti-class antibodies规格： 0.2ml

Anti-NKR/Neurokin B receptor /FITC 荧光素标记神经激肽-B受体抗体IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh BTBD1/HCV NS5A transactivated protein 8 丙毒NS5A反转录蛋白8抗体 规格 0.2ml

ASCL1/MASH1(Achaete-scute homolog 1) 神经母细胞特异性转移因子抗原 0.5mg

Acetyl-Histone H4(K8) 英文名称： 乙酰化组蛋白H4抗体 0.1ml

Rhesus antibody Rh RIPK3 受体结合丝氨酸苏氨酸激酶3抗体 规格 0.1ml

Anti-NKR/Neurokin B receptor /FITC 荧光素标记神经激肽-B受体抗体IgGMulti-class antibodies规格： 0.2ml

人型肝炎IgM(HCV-IgM)ELISA 试剂盒 96T/48T 试剂盒 组装/原装

豚鼠白介素5(IL-5)免疫试剂盒 Guinea pig ierleukin 5,IL-5 ELISA Kit

髓磷脂碱性蛋白(MBP)ELISA试剂盒 ，英文名： MBP ELISA Kit

MouseVitaminB6,VB6ELISA试剂盒小鼠维生素B6(VB6)ELISA试剂盒规格：96T/48T

FishOsteocalcin/Boneglaprotein,OT/BGPELISA试剂盒鱼骨钙素/骨谷氨酸蛋白(OT/BGP)ELISA试剂盒规格：96T/48T

HumanankyrinB,Ank-BELISAKit人锚蛋白B(Ank-B)ELISA试剂盒规格：96T/48T

大鼠同型半胱酸(HA)ELISA试剂盒 ，英文名： HA ELISA Kit

Bovine glucose 6 phosphate dehydrogenase (G6PD) ELISA Kit 牛葡萄糖6磷酸脱氢酶(G6PD)ELISA试剂盒

RatNeonatalThyroxine,NN-T4ELISAKit 大鼠新生甲状腺素(NN-T4)ELISA试剂盒 96T/48T 进口分装

CLIAKitforHO-2(Humanhemeoxygenase2)ELISAKit人血红素氧合酶2规格：48T/96T

细胞PKA激酶活性定量检测试剂盒(A/B/C)20/50次

ELISAKitP-Selectin/CD62P大鼠P选择素规格：48T/96T

细胞骨架结合蛋白2抗体说明书 大鼠纤维蛋白肽B(FPB)ELISA试剂盒 ，英文名： FPB ELISA Kit

Mouse chorionic gonadoopin (CG) ELISA Kit 小鼠绒毛膜激素(CG)ELISA试剂盒

Mousemaixmetalloproteinase5,MMP-5ELISAKit 小鼠基质金属蛋白酶5(MMP-5)ELISA试剂盒 96T/48T 进口分装

CLIAKitforHuman2,3-dinohromboxaneB2,2,3-dinor-TXB2ELISAKit人2,3Dinor烷B2规格：48T/96T

细胞HSV(HERPESSIMPLX)病毒定性检测试剂盒20次

ELISAKitEGF大鼠表皮生长因子规格：48T/96T

抗体的生物素化标记实验要点：

1. 细胞骨架结合蛋白2抗体说明书 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及苯酚基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

抗体的鉴定：

1）细胞骨架结合蛋白2抗体说明书 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。