产品订购信息：
英文名称  Anti-TUB 1 

中文名称   TUB蛋白抗体费用 

别    名  F10B5.4; rd5; TUB 1; TUB; TUB_HUMAN; Tubby homologue; Tubby protein homolog 1; Tubby protein homolog.

浓    度   1mg/1ml

规 格  0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Chicken, Pig, Cow, Horse 

产品类型   一抗  

研究领域    神经生物学 转录调节因子 表观遗传学

蛋白分子量  predicted molecular weight: 56kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human TUB 1 (241-305aa) 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

TUB蛋白抗体费用 产品应用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 In contrast to the rapid early-onset weight gain seen in ob/ob mice (1-3), mutations in the tub gene lead to obesity gradually and strongly resemble late-onset obesity as seen in the human population (4). In addition to excessive deposition of adipose tissue, mice with the tub phenotype also suffer retinal degeneration and neurosensory hearing loss (4-6). The tripartite character of tubby phenotype is strikingly similar to human obesity syndromes such as Alstr鰉 (5) and Bardet-Biedl (6). A candidate for the tub gene has been described (4). A G芓 transversion in this candidate gene eliminates a donor splice site in the 3' coding region resulting in a larger transcript containing an unspliced intron (4). A second prematurely truncated mRNA transcript with the unspliced intron was found to be expressed in the brains of tubby mice at a 2-3 fold higher rate as compared to B6 mice (4). It has been postulated that the phenotypic features of tubby mice can be attributed to cellular apoptosis triggered by the expression of a mutated tub gene (4).

Function : Functions in signal transduction from heterotrimeric G protein-coupled receptors. Binds to membranes containing phosphatidylinositol 4,5-bisphosphate. Can bind DNA (in vitro). May contribute to the regulation of transcription in the nucleus. Could be involved in the hypothalamic regulation of body weight (By similarity). Contribute to stimulation of phagocytosis of apoptotic retinal pigment epithelium (RPE) cells and macrophages.

Subunit : Interacts with GNAQ (By similarity). Interacts with TULP1

Subcellular Location : Cytoplasm. Nucleus. Secreted. Cell membrane. Binds phospholipid and is anchored to the plasma membrane through binding phosphatidylinositol 4,5-bisphosphate. Is released upon activation of phospholipase C. Translocates from the plasma membrane to the nucleus upon activation of guanine nucleotide-binding protein G(q) subunit alpha. Does not have a cleavable signal peptide and is secreted by a non-conventional pathway.

Similarity : Belongs to the TUB family.

Database links : UniProtKB/Swiss-Prot: P50607.1

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M199培养液 250ml 国产

ZNF323 英文名称： 锌指蛋白323抗体 0.1ml

DPPA2 英文名称： 多能发育相关基因2抗体 0.1ml

吸引素抗体 Anti-ATRN 0.1ml

ERK(Extracellular signal-regulated kinase) 原活化蛋白激酶抗原Multi-class antibodies规格： 0.5mg

精氨酸加压素受体2抗体 Anti-AVPR2 0.1ml

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1640培养液 250ml 国产

ZNT8 英文名称： 锌转运体8蛋白抗体 0.2ml

DTNB 英文名称： 肌Dystrobrevin β蛋白抗体 0.2ml

精氨酸加压素受体2抗体 Anti-AVPR2 0.1ml

Human Immunoglobulin M,IgM ELISA Kit 人免疫球蛋白MMulti-class antibodies规格： 48T

Anti-phospho-RUNX1/AML1(Ser249) 磷酸化急性髓细胞1蛋白抗体Multi-class antibodies规格： 0.1ml

Rhesus antibody Rh GRM2/GLUR2 促代谢型谷氨酸受体2抗体 规格 0.1ml

CEA/CD66(Human carcinoembryonic antign) ELISA Kit 人癌胚抗原 96T

1-Oct 英文名称： 阳离子转运器1抗体 0.1ml

phospho-Band3 (Tyr359) 英文名称： 磷酸化红细胞阴离子交换蛋白1抗体 0.1ml

Anti-phospho-RUNX1/AML1(Ser249) 磷酸化急性髓细胞1蛋白抗体Multi-class antibodies规格： 0.1ml

人层连蛋白/板层素(LN)ELISA 试剂盒 96T/48T 试剂盒 组装/原装

豚鼠基质金属蛋白酶抑制因子1(TIMP-1)免疫试剂盒 guinea pig tissue inhibitors of metalloproteinase 1,TIMP-1 ELISA Kit

胎球蛋白A(Fetuin A)ELISA试剂盒 ，英文名： Fetuin A ELISA Kit

Mousetoxicshocksyndrometoxin,TSST-1ELISA试剂盒小鼠毒性休克综合征毒素1(TSST-1)ELISA试剂盒规格：96T/48T

DuckImmunoglobulinE,IgEELISA试剂盒鸭免疫球蛋白E(IgE)ELISA试剂盒规格：96T/48T

HumanAmebiasisELISAKit人阿米巴(Amebiasis)ELISA试剂盒规格：96T/48T

大鼠脱氧酚/脱氧啉(DPD)ELISA试剂盒 ，英文名： DPD ELISA Kit

人游离特异性抗原(fPSA)ELISA检测试剂盒HumanFreeProstateSpecificAigen,fPSAELISAKit 96T/48T

仓鼠基质金属蛋白酶9/明胶酶B(MMP-9/Gelatinase B) 免疫试剂盒 Hamster maix metalloproteinase 9/Gelatinase B,MMP-9 ELISA Kit

CLIAKitforPY(Humanpyridiniumcrosslink/PyriLinks)ELISAKit人交联物规格：48T/96T

石蜡切片脂质过氧化物反式十八碳四烯酸(cis-parinaricacid)荧光染色试剂盒10次

ELISAKitBMP-4人骨成型蛋白4规格：48T/96T

TUB蛋白抗体费用 大鼠腺苷酸环化酶1(AC-1)ELISA试剂盒 ，英文名： AC-1 ELISA Kit

人腺相关病毒(AAV)ELISA检测试剂盒Humanadeno-associatedvirus,AAVELISAKit 96T/48T

大鼠ADM2(ADM2)免疫试剂盒 Rat ADM2,ADM2 ELISA kit

CLIAKitforsCD38(HumanSolubleClusterofdiffereiation38)ELISAKit人可溶性CD38规格：48T/96T

石蜡切片冯库萨(VONKOSSA)钙染色试剂盒50次

ELISAKitMMP-10人基质金属蛋白酶10规格：48T/96T

抗体的生物素化标记实验要点：

1. TUB蛋白抗体费用 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及苯酚基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

抗体的鉴定：

1）TUB蛋白抗体费用 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。