产品订购信息：
英文名称  Anti-TGFB4/LEFTY2 

中文名称   转化生长因子β4抗体说明书 

别    名  EBAF; Endometrial bleeding associated factor (left right determination factor A transforming growth factor beta superfamily); Endometrial bleeding associated factor; Endometrial bleeding-associated factor; Left right determination factor 2; Left right determination factor A; Left-right determination factor 2; Left-right determination factor A; LEFTA; LEFTY 2; LEFTY2; LEFTYA; LFTY2_HUMAN; MGC46222; Protein lefty 2; Protein lefty A; Protein lefty-2; Protein lefty-A; Protein lefty2; PSEC0024; TGF beta 4; TGF-beta-4; TGFB4; Transforming growth factor beta 4; Transforming growth factor beta-4.

浓    度   1mg/1ml

规 格  0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat 

产品类型   一抗  

研究领域     免疫学 信号转导 干细胞 生长因子和激素

蛋白分子量  predicted molecular weight: 32kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human TGFB4 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

转化生长因子β4抗体说明书 产品应用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 LEFTY2 is a member of the TGF-beta family of proteins. LEFTY2 is secreted and plays a role in left-right asymmetry determination of organ systems during development and may also play a role in endometrial bleeding. Mutations in this gene have been associated with left-right axis malformations, particularly in the heart and lungs. Some types of infertility have been associated with dysregulated expression of this gene in the endometrium. Alternative processing of this protein can yield three different products. This gene is closely linked to both a related family member and a related pseudogene.

Function : Required for left-right (L-R) asymmetry determination of organ systems in mammals. May play a role in endometrial bleeding.

Subcellular Location : Secreted.

Tissue Specificity : Mesenchymal cells of the endometrial stroma.

Post-translational modifications : The processing of the protein may also occur at the second R-X-X-R site located at AA 132-135. Processing appears to be regulated in a cell-type specific manner.

DISEASE : Defects in LEFTY2 are the cause of left-right axis malformations (LRAM) [MIM:601877]. The defect includes left pulmonary isomerism, with cardiac anomalies characterized by complete atrioventricular canal defect and hypoplastic left ventricle, and interrupted inferior vena cava.

Similarity : Belongs to the TGF-beta family.

Database links : UniProtKB/Swiss-Prot: O00292.2

Anti-Vimentin/FITC 荧光素标记波形蛋白抗体IgGMulti-class antibodies规格： 0.2ml

CGB(Chromogranin B) 嗜铬粒素B抗原Multi-class antibodies规格： 0.5mg

周期素依赖激酶1抗体 Anti-Cdc2/P34CDC2/CDK1 0.1ml

SUFU/Suppressor of Fused 英文名称： 抑制Hh信号通路蛋白SUFU抗体 0.2ml

ESAT6 英文名称： 结核分枝杆菌分泌性蛋白ESAT6抗体 0.2ml

Rhesus antibody Rh phospho-GADD153 (Ser30) 磷酸化GADD153抗体 规格 0.1ml

CGB(Chromogranin B) 嗜铬粒素B抗原Multi-class antibodies规格： 0.5mg

Anti-SHP2/PTPN11/FITC 荧光素标记蛋白酪氨酸磷酸酶2抗体IgGMulti-class antibodies规格： 0.2ml

ILK-1 (Integrin-linked protein kinase 1) 整合素连接激酶-1Multi-class antibodies规格： 0.5mg

P物质受体抗体 Anti-NK-1/SuRCtance P Receptor 0.1ml

Smad4 英文名称： Smad4抗体 0.1ml

EVA1 英文名称： 髓鞘蛋白P0样蛋白2抗体 0.2ml

Rhesus antibody Rh Phospho-p63 (Ser395) 磷酸化P63抑制基因抗体 规格 0.1ml

ILK-1 (Integrin-linked protein kinase 1) 整合素连接激酶-1Multi-class antibodies规格： 0.5mg

VASH1(Vasohibin 1) 兔抑制蛋白1抗原Multi-class antibodies规格： 0.5mg

Anti-procaspase 3 半胱天冬酶-3酶原抗体Multi-class antibodies规格： 0.2ml

Rhesus antibody Rh PCNA [Proliferation Marker] 增殖细胞核抗原抗体 规格 0.1ml

GRN Kit Human 人 Granulin / GRN / Progranulin ELISA配对抗体 ELISA

TRPM1 英文名称： 瞬时受体电位离子通道蛋白1抗体(M亚家族) 0.1ml

Cdc6 英文名称： 细胞分裂周期蛋白6抗体 0.1ml

Anti-procaspase 3 半胱天冬酶-3酶原抗体Multi-class antibodies规格： 0.2ml

FAM3C Protein Human 重组人 FAM3C / ILEI 蛋白 (Fc 标签)

Ke-3 人细胞

ULBP2 Others Human 人 ULBP2 / N2DL-2 人细胞裂解液 (阳性对照)

MDA-MB-436人腺癌细胞 MDA-MB-436 human breast adenocarcinoma cells L-15培养基+10%FBS

中国仓鼠卵巢细胞K1(亚系克隆);CHO-K1

小鼠雪旺细胞(MSC)(5×105) MDA-MB-231, 人癌细胞 Human

CL-0338G1(发绿色荧光的小鼠胚胎干细胞)5×106cells/瓶×2

ESAM Others Mouse 小鼠 ESAM / Endothelial Cell Adhesion Molecule 人细胞裂解液 (阳性对照)

小鼠肺大动脉内皮细胞完全培养基 100mL

Eca-109人细胞 Human esophageal cancer cell line Eca-109. 1640+10% FBS

IL1B Protein Rat 重组大鼠 IL-1 beta / IL1B 蛋白 (mature form)

NCI-H524(人非小细胞细胞) 5×106cells/瓶×2 HUVEC(人脐内皮细胞)

转化生长因子β4抗体说明书 FAM3C Protein Human 重组人 FAM3C / ILEI 蛋白 (Fc 标签)

Ke-3 人细胞

ULBP2 Others Human 人 ULBP2 / N2DL-2 人细胞裂解液 (阳性对照)

MDA-MB-436人腺癌细胞 MDA-MB-436 human breast adenocarcinoma cells L-15培养基+10%FBS

中国仓鼠卵巢细胞K1(亚系克隆);CHO-K1

小鼠雪旺细胞(MSC)(5×105) MDA-MB-231, 人癌细胞 Human

抗体的生物素化标记实验要点：

1. 转化生长因子β4抗体说明书 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及苯酚基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

抗体的鉴定：

1）转化生长因子β4抗体说明书 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。