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微管蛋白β tubulin抗体 Tubulin β品牌 全：公司提供上万种产品，涵盖了生物试剂，elisa试剂盒，标准品，培养基，原装耗材，抗体、培养基、ATCC细胞等，基本上各种科研所需产品在我司都能找到。

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微管蛋白β tubulin抗体 Tubulin β品牌 英文名称  Anti-tubulin Beta

中文名称  微管蛋白β tubulin抗体 Tubulin β品牌 

别    名 Beta 4 tubulin; Beta 5 tubulin; BetaTubulin; Beta Tubulin; Beta-Tubulin; dJ40E16.7; TUBB; TUBB2; TUBB2A; TUBB5; TBB5_HUMAN; Tubulin beta chain; Tubulin beta-5 chain; tubulin beta 2A.

产品属性：

浓    度  1mg/1ml

规 格  0.1ml/100μg 0.2ml/200μg

抗体来源  Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Rabbit

产品类型   一抗  

研究领域    免疫学 神经生物学 细胞骨架

蛋白分子量  predicted molecular weight: 50kDa 

性    状   Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from human tubulin Beta

亚    型  IgG

纯化方法   affinity purified by Protein A

储 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

微管蛋白β tubulin抗体 Tubulin β品牌 产品应用   WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 kDa. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of beta Tubulin may not be stable in certain cells. For example, expression of tubulin in adipose tissue is very low (Spiegelman and Farmer, Cell, 1982, 29(1):53-60) and therefore beta Tubulin should not be used as loading control for these tissues.

Function : Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

Subunit : Dimer of alpha and beta chains. May interact with RNABP10 (By similarity). Interacts with PIFO. Interacts with MX1 (By similarity).

Subcellular Location : Cytoplasm, cytoskeleton.

Tissue Specificity : Ubiquitously expressed with highest levels in spleen, thymus and immature brain.

Post-translational modifications : Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules (Probable).

Similarity : Belongs to the tubulin family.

Database links :

UniProtKB/Swiss-Prot: P07437.2

Entrez Gene: 203068 Human

Omim: 191130 Human

SwissProt: P07437 Human

SwissProt: P99024 Mouse

SwissProt: P69897 Rat

结构蛋白（Structural Proteins）

tubulin是一种大量存在于哺乳动物脑组织中的微管亚基蛋白，在结构上是由两个极为相近的α和β亚基组成的二聚体、多聚体形成微管细丝，是微管的主要成分。

微管蛋白是球形分子, 有两种类型:α微管蛋白(α-tubulin)货号：bs-0195R和β微管蛋白(β-tubulin), 这两种微管蛋白具有相似的三维结构, 能够紧密地结合成二聚体, 作为微管组装的亚基。 α亚基由450个氨基酸组成, β亚基是由455个氨基酸组成, 这两种亚基有35～40%的氨基酸序列同源, 表明编码它们的基因可能是由同一原始祖先演变而来.

实验流程：

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技术外包服务:

★微管蛋白β tubulin抗体 Tubulin β品牌 分子生物学：质粒抽提、PCR、Q-PCR、RT-PCR、分子生物学：基因合成、引物合成、基因测序、载体构建等

★蛋白工程：原核、哺乳动物蛋白表达系统等

★病毒包装：腺病毒、慢病毒等

★抗体工程：磁珠分选、病理染色、WB、ELISA、IP、IF、IHC、FACS、Confocal等等

★细胞工程：细胞表型分析(凋亡、增殖、周期、迁移、侵袭、修复、克隆形成)、细胞培养、细胞膜制备、稳定细胞株构建、细胞RNAi技术等等。