产地 | 进口、国产 |
品牌 | 上海莼试 |
保存条件 | Store at -20 °C |
货号 | CS11525 |
应用范围 | WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 |
CAS编号 | |
抗体名 | Anti-phospho-TRIM25(Ser100) |
克隆性 | |
靶点 | 详见说明书 |
适应物种 | 详见说明书 |
形态 | 详见说明书 |
宿主 | 详见说明书 |
亚型 | IgG |
标识物 | 详见说明书 |
浓度 | 1mg/1ml% |
免疫原 | KLH conjugated Synthesised phosphopeptide derived from human TRIM25 around the phosphorylation site of Ser100 |
磷酸化雌激素反应指状蛋白抗体费用 英文名称 Anti-phospho-TRIM25(Ser100)
中文名称 磷酸化雌激素反应指状蛋白抗体费用
别 名 TRIM25(phospho S100);EFP; Estrogen responsive finger protein; RNF 147; RNF147; TRI25; TRIM 25; Tripartite motif containing 25; Tripartite motif containing protein 25; Z147; Zinc finger protein 147; Zinc finger protein 147 (estrogen responsive finger protein); ZNF 147; ZNF147.
产品属性:
浓 度 1mg/1ml
规 格 0.1ml/100μg
抗体来源 Rabbit
克隆类型 polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Pig, Cow, Horse, Rabbit
产品类型 一抗 磷酸化抗体
研究领域 免疫学 发育生物学 染色质和核信号
蛋白分子量 predicted molecular weight: 71kDa
性 状 Lyophilized or Liquid
免 疫 原 KLH conjugated Synthesised phosphopeptide derived from human TRIM25 around the phosphorylation site of Ser100
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
磷酸化雌激素反应指状蛋白抗体费用 产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
(石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍 The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc binding domains, a RING, a B box type 1 and a B box type 2, and a coiled coil region. The protein localizes to the cytoplasm. The presence of potential DNA binding and dimerization transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene.
Subunit : Monomer; in the absence of TGF-beta. Homooligomer; in the presence of TGF-beta. Heterotrimer; forms a heterotrimer in the presence of TGF-beta consisting of two molecules of C-terminally phosphorylated SMAD2 or SMAD3 and one of SMAD4 to form the transcriptionally active SMAD2/SMAD3-SMAD4 complex.
Subcellular Location : Cytoplasm. Nucleus.
Similarity : Belongs to the dwarfin/SMAD family.
Contains 1 MH1 (MAD homology 1) domain.
Contains 1 MH2 (MAD homology 2) domain.
实验流程:
全、新、优、品、好四大特点:
磷酸化雌激素反应指状蛋白抗体费用 全:公司提供上万种产品,涵盖了生物试剂,elisa试剂盒,标准品,培养基,原装耗材,抗体、培养基、ATCC细胞等,基本上各种科研所需产品在我司都能找到。
新:产品更新速度较快,基本上每周都有新产品出现。
优:产品质量好,投诉比较少。
好:我公司具有优质的技术团队,产品一旦售出,实验过程中遇到困难可提供在线技术咨询。使您使用产品时没有任何的后顾之忧。
正在热销的相关产品:
Anti-TREM2/FITC 荧光素标记兔抗人、大、小鼠髓系细胞触发受体2抗体IgGMulti-class antibodies规格: 0.2ml
Secretin 分泌素(抗原)Multi-class antibodies规格: 0.5mg
Trop-2抗体 Anti-Trop-2 0.1ml
SCN4B 英文名称: 通道亚基β4抗体 0.2ml
Ephrin B3 英文名称: 酪酸蛋白激酶B3抗体 0.2ml
Rhesus antibody Rh phospho-IRS1(Ser302) 磷酸化受体底物-1抗体 规格 0.1ml
Secretin 分泌素(抗原)Multi-class antibodies规格: 0.5mg
Anti-PRL3/PTP4A3/FITC 荧光素标记磷酸酯酶3蛋白抗体IgGMulti-class antibodies规格: 0.2ml
Mouse Anti-Bovine IgG Whole serum 小鼠抗IgG抗血清Multi-class antibodies规格: 1ml
干扰素-β抗体 Anti-IFN-β Anti-human 0.1ml
Rabbit Anti-Goat IgG 兔抗羊IgG 1mg
FA20A 英文名称: FA20A抗体 0.1ml
Rhesus antibody Rh phospho-RAD9(Ser277) 磷酸化细胞周期检查控制蛋白质抗体 规格 0.1ml
Mouse Anti-Bovine IgG Whole serum 小鼠抗IgG抗血清Multi-class antibodies规格: 1ml
WWOX (WW domain-containing oxidoreductase) 包含氧化还原酶的WW域抗原Multi-class antibodies规格: 0.5mg
Anti-Caveolin-3 细胞质膜微囊蛋白-3抗体Multi-class antibodies规格: 0.2ml
Rhesus antibody Rh PCDHGC4 原钙粘蛋白γC4抗体 规格 0.2ml
PDGFRB Kit Human 人 PDGFRb / CD140b ELISA配对抗体 ELISA
TSPY1 英文名称: 特异定蛋白质编码Y1抗体 0.1ml
Phospho-cdc25A (Ser76) 英文名称: 磷酸化细胞分裂周期蛋白25抗体 0.1ml
Anti-Caveolin-3 细胞质膜微囊蛋白-3抗体Multi-class antibodies规格: 0.2ml
CD70 Protein Rat 重组大鼠 CD70 / CD27L / TNFSF7 蛋白 (Fc 标签)
EJ 人细胞
SPN Others Mouse 小鼠 SPN / CD43 人细胞裂解液 (阳性对照)
SW1116人腺癌细胞 SW1116 human colon adenocarcinoma cells L-15培养基(GIBCO)+10%FBS
人急性淋巴母细胞性细胞;MOLT-4
人脂肪细胞-内脏(HPA-v)(1×106 ) MDA-MB-231(ATCC来源), 人癌细胞 Human
CL-0427RL1(大鼠肺成纤维样细胞)5×106cells/瓶×2
LRP10 Others Human 人 LRP10 人细胞裂解液 (阳性对照)
大鼠角膜成纤维细胞完全培养基 100mL
Pt K1 (NBL-3)袋鼠肾细胞 Pt K1 (NBL-3) kangaroo kidney cells MEM培养基(GIBCO)+10%FBS
IL36A Protein Human 重组人 IL1F6 / IL36 蛋白 (aa 6-158)
NCI-H716(人结直肠腺癌细胞) 5×106cells/瓶×2 MDA-MB-231(癌细胞)
磷酸化雌激素反应指状蛋白抗体费用 CD70 Protein Rat 重组大鼠 CD70 / CD27L / TNFSF7 蛋白 (Fc 标签)
EJ 人细胞
SPN Others Mouse 小鼠 SPN / CD43 人细胞裂解液 (阳性对照)
SW1116人腺癌细胞 SW1116 human colon adenocarcinoma cells L-15培养基(GIBCO)+10%FBS
人急性淋巴母细胞性细胞;MOLT-4
人脂肪细胞-内脏(HPA-v)(1×106 ) MDA-MB-231(ATCC来源), 人癌细胞 Human
技术外包服务:
★磷酸化雌激素反应指状蛋白抗体费用 分子生物学:质粒抽提、PCR、Q-PCR、RT-PCR、分子生物学:基因合成、引物合成、基因测序、载体构建等
★蛋白工程:原核、哺乳动物蛋白表达系统等
★病毒包装:腺病毒、慢病毒等
★抗体工程:磁珠分选、病理染色、WB、ELISA、IP、IF、IHC、FACS、Confocal等等
★细胞工程:细胞表型分析(凋亡、增殖、周期、迁移、侵袭、修复、克隆形成)、细胞培养、细胞膜制备、稳定细胞株构建、细胞RNAi技术等等。