中文名称  雌激素反应锌指蛋白抗体费用 

英文名称  Anti-TRIM25

别    名 EFP; Estrogen responsive finger protein; RNF 147; RNF147; TRI25; TRIM 25; Tripartite motif containing 25; Tripartite motif containing protein 25; Z147; Zinc finger protein 147; Zinc finger protein 147 (estrogen responsive finger protein); ZNF 147; ZNF147; TRI25_HUMAN.

产品属性：

雌激素反应锌指蛋白抗体费用 浓    度  1mg/1ml

规 格  0.2ml/200μg

抗体来源  Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Dog, Cow, Sheep

产品类型   一抗  

研究领域     免疫学 发育生物学 染色质和核信号 锌指蛋白

蛋白分子量  predicted molecular weight: 71kDa 

性    状   Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from human TRIM25

亚    型  IgG

纯化方法   affinity purified by Protein A

储 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

雌激素反应锌指蛋白抗体费用 产品应用   WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc binding domains, a RING, a B box type 1 and a B box type 2, and a coiled coil region. The protein localizes to the cytoplasm. The presence of potential DNA binding and dimerization transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene.

Function : Functions as an ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of DDX58. Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region which is crucial for triggering the cytosolic signal transduction that leads to the production of interferons in response to viral infection. Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated in the regulation of a large spectrum signaling pathway. Mediates estrogen action in various target organs.

Subunit : Interacts (via SPRY domain) with DDX58 (via CARD domain). Interacts (via coiled coil) with influenza A virus NS1 protein; this interaction specifically inhibits TRIM25 multimerization and TRIM25-mediated DDX58 CARD ubiquitination, thereby suppressing DDX58 signal transduction.

Subcellular Location : Cytoplasm.

Tissue Specificity : Ubiquitous.

Post-translational modifications : Auto-ISGylated.

Similarity : Contains 1 B30.2/SPRY domain.

Contains 1 RING-type zinc finger.

Database links : UniProtKB/Swiss-Prot: Q14258.2

实验流程：

全、新、优、品、好四大特点：

雌激素反应锌指蛋白抗体费用 全：公司提供上万种产品，涵盖了生物试剂，elisa试剂盒，标准品，培养基，原装耗材，抗体、培养基、ATCC细胞等，基本上各种科研所需产品在我司都能找到。

新：产品更新速度较快，基本上每周都有新产品出现。

优：产品质量好，投诉比较少。

好：我公司具有优质的技术团队，产品一旦售出，实验过程中遇到困难可提供在线技术咨询。使您使用产品时没有任何的后顾之忧。

技术外包服务:

★雌激素反应锌指蛋白抗体费用 分子生物学：质粒抽提、PCR、Q-PCR、RT-PCR、分子生物学：基因合成、引物合成、基因测序、载体构建等

★蛋白工程：原核、哺乳动物蛋白表达系统等

★病毒包装：腺病毒、慢病毒等

★抗体工程：磁珠分选、病理染色、WB、ELISA、IP、IF、IHC、FACS、Confocal等等

★细胞工程：细胞表型分析(凋亡、增殖、周期、迁移、侵袭、修复、克隆形成)、细胞培养、细胞膜制备、稳定细胞株构建、细胞RNAi技术等等。

正在热销的相关产品：

Anti-UCN/FITC 荧光素标记新型活性因子UCN抗体(小鼠、大鼠)IgGMulti-class antibodies规格： 0.2ml

CCR-3(CC chemokine receptor 3) CC趋化因子受体3抗原Multi-class antibodies规格： 0.5mg

组织蛋白酶B抗体 Anti-cath-B/CB 0.1ml

Synapsin III 英文名称： 神经突触素3抗体 0.2ml

Ephrin A2 英文名称： Ephrin A2抗体 0.1ml

Rhesus antibody Rh phospho-HNF4 (Ser304) 磷酸化肝细胞核因子4α抗体 规格 0.1ml

CCR-3(CC chemokine receptor 3) CC趋化因子受体3抗原Multi-class antibodies规格： 0.5mg

Anti-wtp53/FITC 荧光素标记野生型p53单克隆抗体IgGMulti-class antibodies规格： 0.2ml

Mouse Anti-Goat IgG/ Biotin 生物素化小鼠抗羊IgGMulti-class antibodies规格： 0.3ml

素氧化环化酶2抗体 Anti-COX-2 0.1ml

Rabbit Anti-rat IgG/Bio 生物素标记的兔抗大鼠IgG 0.1ml

FLVCR 英文名称： 病毒C亚类受体蛋白FLVCR抗体 0.2ml

Rhesus antibody Rh PI3K/PI3 kinase p85 alpha subunit 磷脂酰肌醇激酶抗体 规格 0.1ml

Mouse Anti-Goat IgG/ Biotin 生物素化小鼠抗羊IgGMulti-class antibodies规格： 0.3ml

IL-1 Alpha 大鼠白介素-1αMulti-class antibodies规格： 48T

Anti-Doublecortin 双皮质素抗体Multi-class antibodies规格： 0.1ml

Rhesus antibody Rh NEK8 丝氨酸/苏氨酸蛋白激酶NEK8抗体 规格 0.2ml

Human C-Polypeptide,CP ELISA Kit 人C多肽 96T

Tsukushin 英文名称： TSK蛋白抗体 0.2ml

CRTR1 英文名称： CP2相关转录抑制因子1抗体 0.1ml

Anti-Doublecortin 双皮质素抗体Multi-class antibodies规格： 0.1ml

TNFSF12 Protein Rat 重组大鼠 TNFSF12 蛋白 (Fc 标签)

DLD-1 人结直肠腺癌上皮细胞

CTSD Others Human 人 Cathepsin D / CTSD 人细胞裂解液 (阳性对照)

CMVECs微内皮细胞 CMVECs micro vascular endothelial cells DMEM+10%FBS

人导管癌细胞;ZR-75-1

人小梁网细胞(HTMC)(5×105) 3T3-L1, 小鼠前脂肪细胞 Mouse

CL-0435SF126(人细胞)5×106cells/瓶×2

HA Others H12N1 甲型 H12N1 (A/mallard duck/Alberta/342/1983) 血凝素 (Hemagglutinin / HA) 人细胞裂解液 (阳性对照)

大鼠视网膜神经节细胞完全培养基 100mL

HL-7702人正常肝细胞 HL-7702 human normal liver cell 1640+10% FBS

IL1RN Protein Rat 重组大鼠 IL-1RA / IL1RN 蛋白 (Fc 标签)

NCI-H596(人肺腺鳞癌细胞) 5×106cells/瓶×2 cv-1(非洲绿猴肾细胞)

雌激素反应锌指蛋白抗体费用 TNFSF12 Protein Rat 重组大鼠 TNFSF12 蛋白 (Fc 标签)

DLD-1 人结直肠腺癌上皮细胞

CTSD Others Human 人 Cathepsin D / CTSD 人细胞裂解液 (阳性对照)

CMVECs微内皮细胞 CMVECs micro vascular endothelial cells DMEM+10%FBS

人导管癌细胞;ZR-75-1

人小梁网细胞(HTMC)(5×105) 3T3-L1, 小鼠前脂肪细胞 Mouse