技术外包服务:

★磷酸化神经细胞特异性微管蛋白抗体费用 分子生物学：质粒抽提、PCR、Q-PCR、RT-PCR、分子生物学：基因合成、引物合成、基因测序、载体构建等

★蛋白工程：原核、哺乳动物蛋白表达系统等

★病毒包装：腺病毒、慢病毒等

★抗体工程：磁珠分选、病理染色、WB、ELISA、IP、IF、IHC、FACS、Confocal等等

★细胞工程：细胞表型分析(凋亡、增殖、周期、迁移、侵袭、修复、克隆形成)、细胞培养、细胞膜制备、稳定细胞株构建、细胞RNAi技术等等。

实验流程：

磷酸化神经细胞特异性微管蛋白抗体费用 英文名称  Anti-phospho-TUBB3 (Ser172)

中文名称  磷酸化神经细胞特异性微管蛋白抗体费用 

别    名 beta III Tubulin (phospho S172); p-beta III Tubulin (phospho S172); TUBB3(phospho S172); beta 4; MC1R; TBB3_HUMAN; TUBB 3; TUBB 4; TUBB3; TUBB4; Tubulin beta 3 chain; Tubulin beta 4; Tubulin beta III; Tubulin beta-3 chain; Tubulin beta-4 chain; Tubulin beta-III.

产品属性：

浓    度  1mg/1ml

规 格  0.1ml/100μg

抗体来源  Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Rabbit

产品类型   一抗 磷酸化抗体  

研究领域    细胞生物 免疫学 神经生物学 信号转导 细胞类型标志物 细胞骨架

蛋白分子量  predicted molecular weight: 50-55kDa 

性    状   Lyophilized or Liquid

免 疫 原  KLH conjugated Synthesised phosphopeptide derived from human TUBB3 around the phosphorylation site of Ser172

亚    型  IgG

纯化方法   affinity purified by Protein A

储 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

磷酸化神经细胞特异性微管蛋白抗体费用 产品应用   WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 Tubulin is a major cytoskeleton component that has five distinct forms, designated a, b, g, d and e tubulin. a and b tubulins form heterodimers which multimerize to form a microtubule filament. Multiple b Tubulin isoforms (b1, b2, b3, b4, b5, b6 and b8) have been characterized and are expressed in mammalian tissues. b1 and b4 are present throughout the cytosol, b2 is present in the nuclei and nucleoplasm, and b3 is a neuron-specific cytoskeletal protein. g Tubulin forms the gammasome, which is required for nucleating microtubule filaments at the centrosome. Both d Tubulin and e Tubulin are associated with the centrosome. d Tubulin is a homolog of the Chlamydomonas d Tubulin Uni3 and is found in association with the centrioles, whereas e Tubulin localizes to the pericentriolar material. e Tubulin exhibits a cell cycle-specific pattern of localization; first associating with only the older of the centrosomes in a newly duplicated pair, and later associating with both centrosomes.

Function : Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. TUBB3 plays a critical role in proper axon guidance and mantainance.

Subunit : Dimer of alpha and beta chains.

Subcellular Location : Cytoplasm; cytoskeleton.

Tissue Specificity : Expression is primarily restricted to central and peripheral nervous system.

Post-translational modifications : Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.

DISEASE : Defects in TUBB3 are the cause of congenital fibrosis of extraocular muscles type 3A (CFEOM3A) [MIM:600638]. A congenital ocular motility disorder marked by restrictive ophthalmoplegia affecting extraocular muscles innervated by the oculomotor and/or trochlear nerves. It is clinically characterized by anchoring of the eyes in downward gaze, ptosis, and backward tilt of the head. Congenital fibrosis of extraocular muscles type 3 presents as a non-progressive, autosomal dominant disorder with variable expression. Patients may be bilaterally or unilaterally affected, and their oculo-motility defects range from complete ophthalmoplegia (with the eyes fixed in a hypo- and exotropic position), to mild asymptomatic restrictions of ocular movement. Ptosis, refractive error, amblyopia, and compensatory head positions are associated with the more severe forms of the disorder. In some cases the ocular phenotype is accompanied by additional features including developmental delay, corpus callosum agenesis, basal ganglia dysmorphism, facial weakness, polyneuropathy.

Similarity : Belongs to the tubulin family.

Database links : UniProtKB/Swiss-Prot: Q13509.2

全、新、优、品、好四大特点：

磷酸化神经细胞特异性微管蛋白抗体费用 全：公司提供上万种产品，涵盖了生物试剂，elisa试剂盒，标准品，培养基，原装耗材，抗体、培养基、ATCC细胞等，基本上各种科研所需产品在我司都能找到。

新：产品更新速度较快，基本上每周都有新产品出现。

优：产品质量好，投诉比较少。

好：我公司具有优质的技术团队，产品一旦售出，实验过程中遇到困难可提供在线技术咨询。使您使用产品时没有任何的后顾之忧。

正在热销的相关产品：

Anti-CD72/Ly-32/FITC 荧光素标记CD72抗体IgGMulti-class antibodies规格： 0.2ml

NGF- Beta 神经生长因子-β(多肽片断抗原)Multi-class antibodies规格： 0.5mg

细胞因子信号传导抑制蛋白3抗体 Anti-Socs 3 0.2ml

SCYL1BP1 英文名称： SCYL结合蛋白1抗体 0.1ml

EPHB4 英文名称： 酪酸蛋白激酶受体B4抗体 0.1ml

Rhesus antibody Rh Phospho-MAPKAPK2(Ser272) 磷酸化原活化蛋白激酶活化的蛋白激酶2抗体 规格 0.1ml

NGF- Beta 神经生长因子-β(多肽片断抗原)Multi-class antibodies规格： 0.5mg

Anti-PP-2A/FITC 荧光素标记蛋白质磷酸酶-2A抗体IgGMulti-class antibodies规格： 0.2ml

Mouse Anti-goat IgM Whole serum 小鼠抗羊IgM抗血清Multi-class antibodies规格： 1ml

鼠抗人B核蛋白单克隆抗体 Mouse Anti-influenza B virus nucleoprotein Monoclonal 0.1ml

Rabbit anti-horse IgG whole serum 兔抗马IgG抗血清 1ml

FGF6 英文名称： 纤维母细胞生长因子6抗体 0.1ml

Rhesus antibody Rh phospho-RAD9(Ser375) 磷酸化细胞周期检查控制蛋白质抗体 规格 0.1ml

Mouse Anti-goat IgM Whole serum 小鼠抗羊IgM抗血清Multi-class antibodies规格： 1ml

VSIG4 (V-set and immunoglobulin domain-containing protein 4) VSIG4 T淋巴细胞负调节蛋白之一(抗原)Multi-class antibodies规格： 0.5mg

Anti-SIGLEC10/SLG2 唾液酸结合性免疫球蛋白样凝集素抗体Multi-class antibodies规格： 0.2ml

Rhesus antibody Rh OPN/BSP 骨桥蛋白抗体(分泌型磷蛋白1) 规格 0.1ml

IL16 Kit Human 人 IL-16 ELISA配对抗体 ELISA

TTC23 英文名称： 原癌基因8抗体 0.2ml

CD98 英文名称： CD98重链抗原抗体 0.1ml

Anti-SIGLEC10/SLG2 唾液酸结合性免疫球蛋白样凝集素抗体Multi-class antibodies规格： 0.2ml

TNFSF8 Protein Cynomolgus 重组食蟹猴 CD153 / CD30L / TNFSF8 蛋白 (His 标签)

CRT 神经细胞

IFNB1 Others Mouse 小鼠 IFNB1 / IFN-beta / Ierferon beta 人细胞裂解液 (阳性对照)

MHCC-97H人细胞(高转移) MHCC-97H human hepatoma cells (high ansfer) DMEM+10%FBS

人细胞;Hela 229 [HeLa229]

人晶状体上皮细胞 (HLEpiC)( 5×105 ) HUVEC-12, 人脐内皮细胞系 Human

CL-0443SK-N-MC(人神经上皮瘤细胞)5×106cells/瓶×2

NLGN1 Others Mouse 小鼠 Neuroligin 1 / NLGN1 人细胞裂解液 (阳性对照)

大鼠角膜内皮细胞完全培养基 100mL

MDBK (NBL-1)牛肾细胞 MDBK (NBL-1) of bovine kidney cell RPMI-1640(GIBCO)+10%FBS

IL18 Protein Mouse 重组小鼠 IL18 / IL-18 蛋白

NCI-H524(人非小细胞细胞) 5×106cells/瓶×2 MCF7(癌细胞)

磷酸化神经细胞特异性微管蛋白抗体费用 TNFSF8 Protein Cynomolgus 重组食蟹猴 CD153 / CD30L / TNFSF8 蛋白 (His 标签)

CRT 神经细胞

IFNB1 Others Mouse 小鼠 IFNB1 / IFN-beta / Ierferon beta 人细胞裂解液 (阳性对照)

MHCC-97H人细胞(高转移) MHCC-97H human hepatoma cells (high ansfer) DMEM+10%FBS

人细胞;Hela 229 [HeLa229]

人晶状体上皮细胞 (HLEpiC)( 5×105 ) HUVEC-12, 人脐内皮细胞系 Human