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腺泡状软组织结构域蛋白ASPC抗体费用 英文名称  Anti-TUG/ASPC

中文名称  腺泡状软组织结构域蛋白ASPC抗体费用 

别    名 Alveolar soft part sarcoma chromosomal region candidate gene 1 protein; Alveolar soft part sarcoma chromosome region candidate 1 (human); Alveolar soft part sarcoma locus; ASPC; ASPC1_HUMAN; ASPCR 1; ASPCR1; ASPL; ASPS; ASPSCR 1; Aspscr1; FLJ45380; RCC 17; RCC17; renal cell carcinoma gene on chromosome 17; renal cell carcinoma papillary 17; Renal papillary cell carcinoma protein 17; Tether containing a UBX domain for GLUT4; Tether containing UBX domain for GLUT4; TUG; UBX domain containing protein 9; UBX domain protein 9; UBX domain-containing protein 9; UBXD 9; UBXD9; UBXN 9; UBXN9.

产品属性：

浓    度  1mg/1ml

规 格  0.2ml/200μg

抗体来源  Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat

产品类型   一抗  

研究领域     细胞生物 信号转导 生长因子和激素 结合蛋白

蛋白分子量  predicted molecular weight: 60kDa 

性    状   Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from human TUG/ASPC

亚    型  IgG

纯化方法   affinity purified by Protein A

储 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

腺泡状软组织结构域蛋白ASPC抗体费用 产品应用   WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 Glut4 is a twelve pass transmembrane protein (12TM) whose carboxy-terminus may dictate its cellular localization. Aberrant Glut4 expression has been suggested to contribute to such maladies as obesity and diabetes. Glut4 null mice have shown that while functional Glut4 protein is not required for maintaining normal glucose levels, it is necessary for sustained growth, normal cellular glucose, fat metabolism and prolonged longevity. TUG (ASPL in humans) regulates the trafficking of glucose via Glut4. Full-length TUG forms a complex with Glut4 and in 3T3-L1 adipocytes and this complex is present in unstimulated cells and is disassembled by insulin. TUG acts by trapping endocytosed Glut4 and tethering it intracellularly. Insulin mobilizes this pool of retained Glut4 by releasing the tether.

Function : Tethering protein that sequesters GLUT4-containing vesicles in the cytoplasm in the absence of insulin. Modulates the amount of GLUT4 that is available at the cell surface.

Subunit : Interacts with GLUT4 (By similarity).

Subcellular Location : Endomembrane system; Peripheral membrane protein (By similarity).

Tissue Specificity : Ubiquitous. Highly expressed in testis, heart, skeletal muscle and pancreas.

DISEASE : Note=A chromosomal aberration involving ASPSCR1 is found in patients with alveolar soft part sarcoma. Translocation t(X;17)(p11;q25) with TFE3 forms a ASPSCR1-TFE3 fusion protein.

Note=A chromosomal aberration involving ASPSCR1 has been found in two patients with of papillary renal cell carcinoma. Translocation t(X;17)(p11.2;q25).

Similarity : Contains 1 UBX domain.

Database links : UniProtKB/Swiss-Prot: Q9BZE9.1

实验流程：

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★蛋白工程：原核、哺乳动物蛋白表达系统等

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★细胞工程：细胞表型分析(凋亡、增殖、周期、迁移、侵袭、修复、克隆形成)、细胞培养、细胞膜制备、稳定细胞株构建、细胞RNAi技术等等。