抗体的生物素化标记实验要点：

1. 结构蛋白家族3抗体品牌 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及苯酚基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

产品订购信息：
英文名称  Anti-TCTN3/TECT3 

中文名称   结构蛋白家族3抗体品牌 

别    名  C10orf61; Chromosome 10 open reading frame 61; DKFZP564D116; TCTN3; TECT3_HUMAN; Tectonic 3; Tectonic 3 precursor; Tectonic family member 3; Tectonic-3.

浓    度   1mg/1ml

规 格  0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Chicken, Dog, Cow, Rabbit, Sheep 

产品类型   一抗  

研究领域    细胞生物 细胞凋亡 生长因子和激素

蛋白分子量  predicted molecular weight: 64kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human TCTN3/TECT3 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

结构蛋白家族3抗体品牌 产品应用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:100-500 ICC=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 Tect3 is a 607 amino acid single-pass type I membrane protein that belongs to the tectonic family and exists as four alternatively spliced isoforms. Tect3 interacts with MKS1 and may be involved in apoptosis regulation. The gene that encodes Tect3 contains approximately 31,560 bases and maps to human chromosome 10q24.1. Spanning nearly 135 million base pairs and encoding nearly 1,200 genes, chromosome 10 makes up approximately 4.5% of the human genome. Several protein-coding genes, including those that encode chemokines, cadherins, excision repair proteins, early growth response factors (Egrs) and fibroblast growth receptors (FGFRs), are located on chromosome 10. Defects in some of the genes that map to chromosome 10 are associated with Charcot-Marie Tooth disease, Jackson-Weiss syndrome, Usher syndrome, nonsyndromatic deafness, Wolman’s syndrome, Cowden syndrome, Cockayne syndrome, multiple endocrine neoplasia type 2 and porphyria. Tetrahydrobiopterin deficiency and a number of syndromes involving defective skull and facial bone fusion are also linked to chromosome 10.

Function : May be involved in apoptosis regulation.

Subunit : Part of the tectonic-like complex (also named B9 complex) (By similarity).

Subcellular Location : Membrane.

DISEASE : Defects in TCTN3 are the cause of orofaciodigital syndrome 4 (OFD4) [MIM:258860]. A form of orofaciodigital syndrome, a group of heterogeneous disorders characterized by malformations of the oral cavity, face and digits, and associated phenotypic abnormalities that lead to the delineation of various subtypes. OFD4 patients have tongue nodules, multiple frenulae, broad flat nose, hypertelorism, and short rib polydactyly with tibial dysplasia (Majewski syndrome). The presence of severe tibial aplasia differentiates OFD4 from OFD1. Additional features of cystic dysplastic kidneys and brain malformation, including occipital encephalocele, are observed in severely affected patients.

Defects in TCTN3 are the cause of Joubert syndrome 18 (JBTS18) [MIM:614815]. A form of Joubert syndrome, a disorder presenting with cerebellar ataxia, oculomotor apraxia, hypotonia, neonatal breathing abnormalities and psychomotor delay. Neuroradiologically, it is characterized by cerebellar vermian hypoplasia/aplasia, thickened and reoriented superior cerebellar peduncles, and an abnormally large interpeduncular fossa, giving the appearance of a molar tooth on transaxial slices (molar tooth sign). Additional variable features include retinal dystrophy and renal disease. JBTS18 patients have vermis agenesis and the molar tooth sign as well as severe kyphoscoliosis. Other features include intrauterine growth retardation, oral anomalies, micrognathism, polydactyly and camptodactyly, joint laxity, horseshoe kidney, and ventricular septal defect. Note=TCTN3-mutated fibroblasts from JBTS18 patients fail to respond to Shh agonists suggesting that at least some of the defects in affected individuals may be secondary to reduced Shh signaling (PubMed:22883145).

Similarity : Belongs to the tectonic family.

Database links : UniProtKB/Swiss-Prot: Q6NUS6.2

抗体的鉴定：

1）结构蛋白家族3抗体品牌 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。

Anti-TP II A/TOP2a/FITC 荧光素标记DNA拓普西异构酶ⅡA抗体IgGMulti-class antibodies规格： 0.2ml

SAP-1 (Synapse associated protein-I) peptide 突触相关蛋白-1(多肽抗原)Multi-class antibodies规格： 0.5mg

酪氨酸激酶A抗体 Anti-TrK A 0.1ml

SLU7 英文名称： SLU7蛋白抗体 0.2ml

EN2 英文名称： 同源盒蛋白转录因子EN2抗体 0.1ml

Rhesus antibody Rh phospho-IRS1(Ser612) 磷酸化受体底物-1抗体 规格 0.1ml

SAP-1 (Synapse associated protein-I) peptide 突触相关蛋白-1(多肽抗原)Multi-class antibodies规格： 0.5mg

anti-Rarres2/TIG2/FITC 荧光素标记视黄酸受体应答蛋白2抗体IgGMulti-class antibodies规格： 0.2ml

Nociceptin receptor 孤菲肽受体(痛敏肽受体)(抗原)Multi-class antibodies规格： 0.5mg

信号转导和转录激活因子2抗体 Anti-STAT2 0.2ml

S100A11 英文名称： S100钙结合蛋白A11抗体 0.1ml

EpCAM 英文名称： 上皮特异性抗原抗体 0.1ml

Rhesus antibody Rh Phospho-MAP2 (Thr1620/1623) 磷酸化微管相关蛋白2抗体 规格 0.1ml

Nociceptin receptor 孤菲肽受体(痛敏肽受体)(抗原)Multi-class antibodies规格： 0.5mg

PAR-1 (Protease-activated receptors-1) 蛋白酶激活受体-1抗原Multi-class antibodies规格： 0.5mg

Anti-phospho-ATF2(Ser490/498) 磷酸化活化复制因子2抗体Multi-class antibodies规格： 0.1ml

Rhesus antibody Rh phospho-APG4B(Ser309) 磷酸化自噬相关蛋白4B抗体 规格 0.1ml

S100 A4 浓缩液 0.1ml 进口分装

UBE2E2 英文名称： 泛素蛋白连接酶2E2抗体 0.2ml

DHRS4L2 英文名称： 短链脱氢酶/还原酶家族4样2蛋白抗体 0.2ml

Anti-phospho-ATF2(Ser490/498) 磷酸化活化复制因子2抗体Multi-class antibodies规格： 0.1ml

IL3 Protein Rat 重组大鼠 IL3 / ierleukin 3 蛋白 (His 标签)

II型胶原酶(含100mL酶解缓冲液) 10mL

HA Others H3N2 甲型 H3N2 (A/Perth/16/2009) 血凝素 (Hemagglutinin / HA) 人细胞裂解液 (阳性对照)

TE-1人细胞 Human esophageal cancer cell line TE-1. RPMI-1640(GIBCO)+10%FBS

SV40转化的非洲绿猴肾细胞;COS-1

人毛发干细胞(HHDPC)( 5×105 ) MLF, 小鼠淋巴成纤维细胞 Mouse

CM-H077人心室肌细胞完全培养基100mL

CGB5 Others Human 人 CGB5 人细胞裂解液 (阳性对照)

人外根鞘细胞HHORSC

NCL-H548细胞，人*癌细胞 人癌细胞,MDA-MB-231细胞 巨噬细胞培养基MaM

大额牛肾细胞;BFR-K1

LAYN Others Human 人 Layilin 人细胞裂解液 (阳性对照)

结构蛋白家族3抗体品牌 IL3 Protein Rat 重组大鼠 IL3 / ierleukin 3 蛋白 (His 标签)

II型胶原酶(含100mL酶解缓冲液) 10mL

HA Others H3N2 甲型 H3N2 (A/Perth/16/2009) 血凝素 (Hemagglutinin / HA) 人细胞裂解液 (阳性对照)

TE-1人细胞 Human esophageal cancer cell line TE-1. RPMI-1640(GIBCO)+10%FBS

SV40转化的非洲绿猴肾细胞;COS-1

人毛发干细胞(HHDPC)( 5×105 ) MLF, 小鼠淋巴成纤维细胞 Mouse