全、新、优、品、好四大特点：

细胞凋亡抑制因子抗体规格 全：公司提供上万种产品，涵盖了生物试剂，elisa试剂盒，标准品，培养基，原装耗材，抗体、培养基、ATCC细胞等，基本上各种科研所需产品在我司都能找到。

新：产品更新速度较快，基本上每周都有新产品出现。

优：产品质量好，投诉比较少。

好：我公司具有优质的技术团队，产品一旦售出，实验过程中遇到困难可提供在线技术咨询。使您使用产品时没有任何的后顾之忧。

细胞凋亡抑制因子抗体规格 英文名称  Anti-Survivin

中文名称  细胞凋亡抑制因子抗体规格 

别    名 API4; API-4; API 4; Apoptosis Inhibitor 4; Apoptosis inhibitor survivin; Apoptosis inhibitor4; Baculoviral IAP repeat containing 5; Baculoviral IAP repeat containing protein 5; Baculoviral IAP repeat-containing protein 5; BIRC 5; BIRC5; BIRC-5; EPR 1; EPR-1; IAP4; IAP-4; IAP 4; SVV; TIAP; BIRC5_HUMAN.

产品属性：

浓    度  1mg/1ml

规 格  0.2ml/200μg

抗体来源  Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Dog, Pig, Cow

产品类型   一抗  

研究领域    细胞生物 细胞凋亡

蛋白分子量  predicted molecular weight: 18.5kDa 

性    状   Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from human BIRC5 C-terminus

亚    型  IgG

纯化方法   affinity purified by Protein A

储 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

细胞凋亡抑制因子抗体规格 产品应用   WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 Shepherdin (Survivin variant; baculoviral IAP repeat-containing protein 5 isoform 3; BIRC5). Baculoviral inhibition of apoptosis protein repeat domain; Found in inhibitors of apoptosis proteins (IAPs) and other proteins. In higher eukaryotes, BIR domains inhibit apoptosis by acting as direct inhibitors of the caspase family of protease enzymes. In yeast, BIR domains are involved in regulating cytokinesis. This novel fold is stabilized by zinc tetrahedrally coordinated by one histidine and three cysteine residues and resembles a classical zinc finger.

Survivin is an anti-apoptotic protein, expressed in the G2/M cell cycle phase. At the onset of mitosis, survivin associates with microtubules of the mitotic spindle in a specific and saturable reaction that is regulated by microtubule dynamics. Disruption of survivin-microtubule interactions results in loss of survivin's anti-apoptotic function and increased caspase 3 activity. Survivin is also abundantly expressed in brain tissues (astrocytes and some neurons) of adult rats following traumatic brain injury. Survivin has been found co-expressed with NeuN (mature neuronal marker) and PCNA (a cell cycle protein. Survivin might affect regulation of neural cell proliferative responses after brain injury. Survivin is also an inhibitor of apoptosis (IAP), or programmed cell death, that is selectively overexpressed in common human cancers.

Function : Multitasking protein that has dual roles in promoting cell proliferation and preventing apoptosis. Component of a chromosome passage protein complex (CPC) which is essential for chromosome alignment and segregation during mitosis and cytokinesis. Acts as an important regulator of the localization of this complex; directs CPC movement to different locations from the inner centromere during prometaphase to midbody during cytokinesis and participates in the organization of the center spindle by associating with polymerized microtubules. The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. May counteract a default induction of apoptosis in G2/M phase. The acetylated form represses STAT3 transactivation of target gene promoters. May play a role in neoplasia. Inhibitor of CASP3 and CASP7. Isoform 2 and isoform 3 do not appear to play vital roles in mitosis. Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform.

Subunit : Monomer or homodimer. Exists as a homodimer in the apo state and as a monomer in the CPC-bound state. The monomer protects cells against apoptosis more efficiently than the dimer. Only the dimeric form is capable of enhancing tubulin stability in cells. When phosphorylated, interacts with HBXIP; the resulting complex binds pro-CASP9, as well as active CASP9, but much less efficiently. Component of the chromosomal passenger complex (CPC) composed of at least BIRC5/survivin, CDCA8/borealin, INCENP, AURKB and AURKC. Interacts with JTB. Interacts with CDCA8 and INCENP; interaction is direct. Interacts with EVI5. Interacts with GTP-bound RAN in both the S and M phases of the cell cycle. Interacts with USP9X. Interacts with tubulin. Interacts with BIRC2/c-IAP1. The acetylated form at Lys-129 interacts with STAT3. The monomeric form deacetylated at Lys-129 interacts with XPO1/CRM1. The monomeric form interacts with XIAP/BIRC4. Both the dimeric and monomeric form can interact with DIABLO/SMAC. Interacts with BIRC6/bruce.

Subcellular Location : Cytoplasm. Nucleus. Chromosome. Chromosome, centromere. Cytoplasm, cytoskeleton, spindle. Chromosome, centromere, kinetochore. Midbody. Note=Localizes on chromosome arms and inner centromeres from prophase through metaphase. Localizes to kinetochores in metaphase, distributes to the midzone microtubules in anaphase and at telophase, localizes exclusively to the midbody. Colocalizes with AURKB at mitotic chromosomes. Acetylation at Lys-129 directs its localization to the nucleus by enhancing homodimerization and thereby inhibiting XPO1/CRM1-mediated nuclear export.

Tissue Specificity : Expressed only in fetal kidney and liver, and to lesser extent, lung and brain. Abundantly expressed in adenocarcinoma (lung, pancreas, colon, breast, and prostate) and in high-grade lymphomas. Also expressed in various renal cell carcinoma cell lines.

Post-translational modifications : Ubiquitination is required for centrosomal targeting.

In vitro phosphorylation at Thr-117 by AURKB prevents interaction with INCENP and localization to mitotic chromosomes. Phosphorylation at Thr-48 by CK2 is critical for its mitotic and anti-apoptotic activities.

Acetylation at Lys-129 by CBP results in its homodimerization, while deacetylation promotes the formation of monomers which heterodimerize with XPO1/CRM1 which facilitates its nuclear export. The acetylated form represses STAT3 transactivation. The dynamic equilibrium between its acetylation and deacetylation at Lys-129 determines its interaction with XPO1/CRM1, its subsequent subcellular localization, and its ability to inhibit STAT3 transactivation.

Similarity : Belongs to the IAP family.

Contains 1 BIR repeat.

为Survivin家族成员，又称：细胞凋亡抑制因子4，主要用于方面的研究。

实验流程：

正在热销的相关产品：

Anti-phospho-SHC (Tyr349) /FITC 荧光素标记磷酸化SH2结构域转化蛋白1抗体IgGMulti-class antibodies规格： 0.2ml

Apaf-1 (Apoptosis protease activating factor-1)(NT) 凋亡蛋白活性因子-1(抗原)Multi-class antibodies规格： 0.5mg

中分子量神经丝蛋白抗体 Anti-NF-M 0.1ml

Stra8 英文名称： 视黄酸激活基因8抗体 0.1ml

ELK1 英文名称： 细胞转录因子ELK1抗体 0.1ml

Rhesus antibody Rh Phospho-p90RSK (Thr573) 磷酸化丝氨酸/苏氨酸激酶p90RSK蛋白抗体 规格 0.1ml

Apaf-1 (Apoptosis protease activating factor-1)(NT) 凋亡蛋白活性因子-1(抗原)Multi-class antibodies规格： 0.5mg

Anti-S100-A8/MRP8/CFAG/FITC 荧光素标记钙结合蛋白S100A8抗体IgGMulti-class antibodies规格： 0.2ml

PAR-2 AP (Protease-activated receptors-2 Activating polypeptide) 蛋白酶激活受体-2激活肽Multi-class antibodies规格： 0.5mg

巨噬细胞移动抑制因子抗体 Anti-MIF 0.1ml

SCN5A 英文名称： 电压门控通道5α抗体 0.2ml

FBXO21 英文名称： F-box蛋白21抗体 0.2ml

Rhesus antibody Rh Phospho-PDPK1(Tyr9) 磷酸化3磷酸肌醇依赖性蛋白激酶1抗体 规格 0.1ml

PAR-2 AP (Protease-activated receptors-2 Activating polypeptide) 蛋白酶激活受体-2激活肽Multi-class antibodies规格： 0.5mg

PCNA(phosphos Dyr211)peptide 磷酸化增殖细胞核多肽抗原Multi-class antibodies规格： 0.5mg

Anti-ATF6 活化转录因子6抗体Multi-class antibodies规格： 0.1ml

Rhesus antibody Rh phospho-Androgen Receptor (Ser212) 磷酸化雄激素受体抗体 规格 0.1ml

Pancreatic Polypeptide 浓缩液 0.1ml 进口分装

UCHL3 英文名称： 泛素硫酯酶L3抗体 0.2ml

Dmrta1 英文名称： 性发育转录因子蛋白DMO抗体 0.1ml

Anti-ATF6 活化转录因子6抗体Multi-class antibodies规格： 0.1ml

IL12B Protein Human 重组人 IL12B / P40 蛋白 (Fc 标签)

小鼠成骨细胞完全培养基 100mL

JAM3 Others Human 人 JAM3 / JAM-C 人细胞裂解液 (阳性对照)

P388D1小鼠淋巴样瘤细胞 P388D1 mouse lymphoid tumor cells RPMI-1640(GIBCO)+90%马血清+10%FBS

CM-R060大鼠肾动脉内皮细胞完全培养基100mL

人脑微内皮细胞 (HBMEC) ( 5×105 ) 大鼠主动脉平滑肌细胞 细胞名称

CM-H135人脉络膜微细胞完全培养基100mL

DPYS Others Human 人 DPYS / Dihydropyrimidinase 杆状病毒-昆虫细胞裂解液 (阳性对照)

人*内皮细胞HSEC

非洲绿猴肾细胞(SV40转化);COS-1 [COS1] 转染腺病毒E1A基因的人肾上皮细胞，293细胞 QGY-7703(细胞)

人细胞;A549 [A-549]

FAS Others Mouse 小鼠 FAS / TNFRSF6 / CD95 人细胞裂解液 (阳性对照)

细胞凋亡抑制因子抗体规格 IL12B Protein Human 重组人 IL12B / P40 蛋白 (Fc 标签)

小鼠成骨细胞完全培养基 100mL

JAM3 Others Human 人 JAM3 / JAM-C 人细胞裂解液 (阳性对照)

P388D1小鼠淋巴样瘤细胞 P388D1 mouse lymphoid tumor cells RPMI-1640(GIBCO)+90%马血清+10%FBS

CM-R060大鼠肾动脉内皮细胞完全培养基100mL

人脑微内皮细胞 (HBMEC) ( 5×105 ) 大鼠主动脉平滑肌细胞 细胞名称

技术外包服务:

★细胞凋亡抑制因子抗体规格 分子生物学：质粒抽提、PCR、Q-PCR、RT-PCR、分子生物学：基因合成、引物合成、基因测序、载体构建等

★蛋白工程：原核、哺乳动物蛋白表达系统等

★病毒包装：腺病毒、慢病毒等

★抗体工程：磁珠分选、病理染色、WB、ELISA、IP、IF、IHC、FACS、Confocal等等

★细胞工程：细胞表型分析(凋亡、增殖、周期、迁移、侵袭、修复、克隆形成)、细胞培养、细胞膜制备、稳定细胞株构建、细胞RNAi技术等等。