抗体的生物素化标记实验要点：

1. 5-羟色胺转运蛋白抗体说明书 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及苯酚基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

产品订购信息：
英文名称  Anti-SLC6A4/5-HTT 

中文名称   5-羟色胺转运蛋白抗体说明书 

别    名  5HT transporter; 5-HTT; 5-HTTLPR; 5HTT; hSERT; HTT; OCD1; SERT; SERT1; SC6A4_HUMAN; SERT; SLC6A4; Sodium dependent serotonin transporter; Sodium-dependent serotonin transporter; Solute carrier family 6 member 4; sodium-dependent serotonin transporter; 5-hydroxytryptamine transporter; 5HT transporter; Na+/Cl- dependent serotonin transporter.

浓    度   1mg/1ml

规 格  0.1ml/100μg 0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Sheep, Guinea Pig 

产品类型   一抗  

研究领域     细胞生物 神经生物学 信号转导 通道蛋白 细胞膜受体 药物及化合物 转运蛋白

蛋白分子量  predicted molecular weight: 70kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human 5-HTT C-terminus 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

5-羟色胺转运蛋白抗体说明书 产品应用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 This gene encodes an integral membrane protein that transports the neurotransmitter serotonin from synaptic spaces into presynaptic neurons. The encoded protein terminates the action of serotonin and recycles it in a sodium-dependent manner. This protein is a target of psychomotor stimulants, such as amphetamines and cocaine, and is a member of the sodium:neurotransmitter symporter family. A repeat length polymorphism in the promoter of this gene has been shown to affect the rate of serotonin uptake and may play a role in sudden infant death syndrome, aggressive behavior in Alzheimer disease patients, and depression-susceptibility in people experiencing emotional trauma. [provided by RefSeq, Jul 2008].

Function : Serotonin transporter whose primary function in the central nervous system involves the regulation of serotonergic signaling via transport of serotonin molecules from the synaptic cleft back into the pre-synaptic terminal for re-utilization. Plays a key role in mediating regulation of the availability of serotonin to other receptors of serotonergic systems. Terminates the action of serotonin and recycles it in a sodium-dependent manner.

Subunit : Monomer or homooligomer (By similarity). Interacts with TGFB1I1. Interacts (via sialylated form) with MYH9. Interacts with SEC23A, SEC24C and INADL. Interacts with NOS1; the interaction may diminish the cell surface localization of SERT in the brain and, correspondingly, reduce serotonin reuptake. Interacts with filamentous actin and STX1A (By similarity). Interacts (via C-terminus) with VIM. Interacts (via C-terminus) with SCAMP2; the interaction is direct and retains transporter molecules intracellularly. Interacts with RAB4 (GTP-bound form); the interaction retains transporter molecules intracellularly.

Subcellular Location : Cell membrane; Multi-pass membrane protein. Endomembrane system; Multi-pass membrane protein. Endosome membrane; Multi-pass membrane protein.

Tissue Specificity : Expressed in platelets (at protein level).

Post-translational modifications : Glycosylated; modification with sialylated N-glycans is a requirement for transporters to associate with each other and to function as homooligomeric forms (By similarity).

Phosphorylation at Thr-276 increases 5-HT uptake and is required for cGMP-mediated SERT regulation (By similarity). Phosphorylation upon PKC stimulation modifies the SERT distribution and density in the membrane, and diminishes the uptake capacity.

Similarity : Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A4 subfamily.

Database links : UniProtKB/Swiss-Prot: P31645.1

SERT是一种钠依赖单胺转运蛋白,由630个氨基酸组成,相对分子质量为:70KDa

抗体的鉴定：

1）5-羟色胺转运蛋白抗体说明书 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。

PROG ELISA Kit 鱼孕激素/孕酮Multi-class antibodies规格： 48T

Anti-DAPK2/FITC 荧光素标记凋亡相关蛋白激酶2抗体IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh CYP4A22 细胞色素P4504A22抗体(脂肪酸Ω羟化酶) 规格 0.2ml

Goat IgG/Cy5.5 荧光素Cy5.5标记羊IgG(细胞流式同型对照) 0.1ml

Elastin 英文名称： 项韧带弹性蛋白抗体 0.1ml

Rhesus antibody Rh ZAP-70 zeta相关蛋白70抗体 规格 0.1ml

Anti-DAPK2/FITC 荧光素标记凋亡相关蛋白激酶2抗体IgGMulti-class antibodies规格： 0.2ml

Anti-TNFAIP1/FITC 荧光素标记坏死因子α诱导蛋白1抗体IgGMulti-class antibodies规格： 0.2ml

PDGF-R-A 血小板源性生长因子受体-A(抗原)Multi-class antibodies规格： 0.5mg

金属蛋白酶组织抑制因子-4抗体 Anti-TIMP-4 0.1ml

5HT3D Receptor 英文名称： 5-羟色胺受体3D抗体 0.2ml

ENAH 英文名称： ENAH蛋白抗体 0.2ml

Rhesus antibody Rh phospho-KCND2/Kv4.2(Thr602) 磷酸化电压门控性通道蛋白Kv4.2抗体 规格 0.1ml

PDGF-R-A 血小板源性生长因子受体-A(抗原)Multi-class antibodies规格： 0.5mg

phospho-p38 ERK/MAPK14 (pThr180/pTyr182) peptide 磷酸化原活化蛋白激酶p38抗原Multi-class antibodies规格： 0.5mg

Anti-MASH1 神经母细胞特异性转移因子抗体Multi-class antibodies规格： 0.1ml

Rhesus antibody Rh phospho-AQP2(Ser269) 磷酸化水通道蛋白2抗体 规格 0.1ml

Tgase 浓缩液 0.1ml 进口分装

UEVLD 英文名称： 泛素结合酶E2样蛋白抗体 0.2ml

DIRC2 英文名称： 干扰蛋白2抗体 0.2ml

Anti-MASH1 神经母细胞特异性转移因子抗体Multi-class antibodies规格： 0.1ml

IL12A & IL12B Protein Mouse 重组小鼠 IL-12 (IL12A & IL12B Heterodimer) 蛋白

小鼠细胞完全培养基 100mL

HA Others H10N9 甲型 H10N9 (A/duck/HongKong/562/1979) 血凝素HA1 (Hemagglutinin) 人细胞裂解液 (阳性对照)

P3X63Ag8小鼠细胞 Mouse myeloma cell line P3X63Ag8 DMEM培养基+10%FBS

CM-R108大鼠脑微内皮细胞完全培养基100mL

UMR-106(大鼠细胞) 5×106cells/瓶×2 HUVEC-12, 人脐内皮细胞系

CM-M012小鼠肺动脉成纤维细胞完全培养基100mL

TMEM27 Others Human 人 TMEM27 人细胞裂解液 (阳性对照)

软骨细胞培养基CM

C-33A细胞，人子细胞 兔主动脉平滑肌细胞,CCC-SMC-1细胞 人表皮角质细胞-胎儿HEK-f

HFSF(人胚胎眼巩膜成纤维细胞) 5×106cells/瓶×2

hMoCD14+-PB single donorpre-screened Pellet 人类外周血CD14+单核细胞团块单一来源，预选型 > 1 mio.cells 人脊髓星形胶质细胞RNAHA-sp miRNA5 μg

5-羟色胺转运蛋白抗体说明书 IL12A & IL12B Protein Mouse 重组小鼠 IL-12 (IL12A & IL12B Heterodimer) 蛋白

小鼠细胞完全培养基 100mL

HA Others H10N9 甲型 H10N9 (A/duck/HongKong/562/1979) 血凝素HA1 (Hemagglutinin) 人细胞裂解液 (阳性对照)

P3X63Ag8小鼠细胞 Mouse myeloma cell line P3X63Ag8 DMEM培养基+10%FBS

CM-R108大鼠脑微内皮细胞完全培养基100mL

UMR-106(大鼠细胞) 5×106cells/瓶×2 HUVEC-12, 人脐内皮细胞系