抗体的生物素化标记实验要点：

1. 转绿激活蛋白SRCAP抗体规格 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及苯酚基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

产品订购信息：
英文名称  Anti-SRCAP 

中文名称   转绿激活蛋白SRCAP抗体规格 

别    名  DOMO1; EAF1; Helicase SRCAP; Snf2 related CBP activator protein; SWR1; SRCAP_HUMAN.

浓    度   1mg/1ml

规 格  0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, Sheep 

产品类型   一抗  

研究领域    细胞生物 神经生物学 信号转导 表观遗传学

蛋白分子量  predicted molecular weight: 343kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human SRCAP 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

转绿激活蛋白SRCAP抗体规格 产品应用    ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 SRCAP (Snf2-related CREBBP activator protein), also known as EAF1, SWR1, or DOMO1, is a 3,230 amino acid protein that belongs to a family of helicases and contains one HSA domain, one helicase C-terminal domain, one helicase ATP-binding domain and three A.T hook DNA-binding domains. Localized to the nucleus, SRCAP functions as a catalytic component of the SRCAP complex, a multi-protein structure that mediates the ATP-dependent exchange of histone dimers for nucleosomal histones, an event that regulates the transcription of select genes via chromatin remodeling. Additionally, the SRCAP complex acts as a coactivator for steroid receptor-mediated transcription, Notch-mediated transcription and CREB-mediated transcription. SRCAP is expressed as multiple alternatively spliced isoforms and is subject to DNA damage-dependent phosphorylation by ATM or ATR.

Function : Catalytic component of the SRCAP complex which mediates the ATP-dependent exchange of histone H2AZ/H2B dimers for nucleosomal H2A/H2B, leading to transcriptional regulation of selected genes by chromatin remodeling. Acts as a coactivator for CREB-mediated transcription, steroid receptor-mediated transcription, and Notch-mediated transcription (from SwissProt).

Subunit : Interacts with CREBBP and EP300. May be part of a complex containing SRCAP, CREBBP, CARM1 and GRIP1. Component of the chromatin-remodeling SRCAP complex composed of at least SRCAP, DMAP1, RUVBL1, RUVBL2, ACTL6A, YEATS4, VPS72, ACTR6 and ZNHIT1. Component of a NuA4-related complex which contains EP400, TRRAP/PAF400, SRCAP, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, actin, ACTL6A/BAF53A, VPS72 and YEATS4/GAS41. Interacts with hepatitis C virus (HCV) NS5A and human adenovirus 2 DBP.

Subcellular Location : Nuclear

Post-translational modifications : Phosphorylated upon DNA damage, probably by ATM or ATR.

DISEASE : Defects in SRCAP are the cause of Floating-Harbor syndrome (FLHS) [MIM:136140]. A rare genetic disorder characterized by proportionate short stature, delayed bone age, delayed speech development, and typical facial features. The face is triangular with deep-set eyes, long eyelashes, bulbous nose, wide columella, short philtrum, and thin lips.

Similarity : Belongs to the SNF2/RAD54 helicase family. SWR1 subfamily.

Contains 3 A.T hook DNA-binding domains.

Contains 1 helicase ATP-binding domain.

Contains 1 helicase C-terminal domain.

Contains 1 HSA domain.

Database links : UniProtKB/Swiss-Prot: Q6ZRS2.3

抗体的鉴定：

1）转绿激活蛋白SRCAP抗体规格 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。

Anti-Phospho-Stat2 (Tyr690)/FITC 荧光素标记磷酸化信号转导和转录激活因子2抗体IgGMulti-class antibodies规格： 0.2ml

Ang- II (Angiotensin II ) 紧张素II抗原Multi-class antibodies规格： 0.5mg

磷脂酰肌醇激酶抗体 Anti-PI3K/P85 alpha 0.1ml

14-3-3 Tau 英文名称： 14-3-3τ蛋白抗体 0.1ml

EGFR 英文名称： 表皮生长因子受体抗体 0.1ml

Rhesus antibody Rh phospho-MYL9(Thr19) 磷酸化肌球蛋白轻链9抗体 规格 0.1ml

Ang- II (Angiotensin II ) 紧张素II抗原Multi-class antibodies规格： 0.5mg

Anti-TRAF1/FITC 荧光素标记坏死因子受体相关因子1抗体IgGMulti-class antibodies规格： 0.2ml

PSD-95 突触后密度蛋白95(抗原)Multi-class antibodies规格： 0.5mg

坏死因子受体相关因子6抗体 Anti-TRAF6 0.2ml

SPRR3 英文名称： 角质蛋白β抗体 0.2ml

ELAVL2 英文名称： ELAVL2蛋白抗体 0.2ml

Rhesus antibody Rh phospho-IRS1(Tyr895) 磷酸化受体底物-1抗体 规格 0.1ml

PSD-95 突触后密度蛋白95(抗原)Multi-class antibodies规格： 0.5mg

ROCK1 (Rho-associated coiled-coil containing protein kinase 1) Rho相关蛋白激酶1抗原Multi-class antibodies规格： 0.5mg

Anti-Phospho-Bad(Ser112) 磷酸化相关死亡促进因子抗体Multi-class antibodies规格： 0.1ml

Rhesus antibody Rh PHKA1 磷酸激酶α1抗体 规格 0.2ml

Keratin6 浓缩液 0.1ml 进口分装

URAT1/SLC22A11 英文名称： 尿酸盐重吸收转运子1抗体 0.1ml

Phospho-CD18 (Thr758) 英文名称： 磷酸化整合素β2/Integrin β2抗体 0.1ml

Anti-Phospho-Bad(Ser112) 磷酸化相关死亡促进因子抗体Multi-class antibodies规格： 0.1ml

LIF Protein Mouse 重组小鼠 LIF 蛋白 (Fc 标签)

小鼠肝窦内皮细胞完全培养基 100mL

IgG2a Others Mouse 小鼠 IgG2a-Fc 人细胞裂解液 (阳性对照)

RAG小鼠肾腺癌细胞 RAG mice renal adenocarcinoma cells MEM培养基(GIBCO)+10%FBS

CL-0103Hep G2(人细胞)5×106cells/瓶×2

RTE(大鼠气管上皮细胞) 5×106cells/瓶×2 A375(人类恶性)

CM-M087小鼠软骨细胞完全培养基100mL

IL2RB Others Mouse 小鼠 CD122 / IL2RB / IL2 Receptor beta 人细胞裂解液 (阳性对照)

动物上皮细胞培养基EpiCM-a-prf

NCI-H1299细胞，人细胞 人胚肾上皮细胞,HK-2C细胞 真皮微内皮细胞Many types of cells包装：5 × 105次方(1ml)

猪肾细胞;LLC-PK1

PRSS8 Others Human 人 Prostasin / Prss8 人细胞裂解液 (阳性对照)

转绿激活蛋白SRCAP抗体规格 LIF Protein Mouse 重组小鼠 LIF 蛋白 (Fc 标签)

小鼠肝窦内皮细胞完全培养基 100mL

IgG2a Others Mouse 小鼠 IgG2a-Fc 人细胞裂解液 (阳性对照)

RAG小鼠肾腺癌细胞 RAG mice renal adenocarcinoma cells MEM培养基(GIBCO)+10%FBS

CL-0103Hep G2(人细胞)5×106cells/瓶×2

RTE(大鼠气管上皮细胞) 5×106cells/瓶×2 A375(人类恶性)