产品订购信息：
英文名称  Anti-Smac/DIABLO 

中文名称   线粒体促凋亡蛋白抗体规格 

别    名  0610041G12Rik; DBLOH_HUMAN; DBOH; DBOH; diablo; Diablo homolog (Drosophila); Diablo homolog (Drosophila); Diablo homolog; Diablo homolog mitochondrial; DIABLO S; DIABLO S; Direct IAP binding protein with low pI; Direct IAP binding protein with low pI; Direct IAP-binding protein with low pI; FLJ10537; FLJ25049; mitochondrial; Mitochondrial Smac protein; Second mitochondria derived activator of caspase; Second mitochondria derived activator of caspase; Second mitochondria-derived activator of caspase; SMAC 3; Smac; Smac protein; Smac protein; SMAC3; SMAC3;

浓    度   1mg/1ml

规 格  0.1ml/100μg 0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse 

产品类型   一抗  

研究领域     细胞生物 细胞凋亡 线粒体

蛋白分子量  predicted molecular weight: 21kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human Smac C-terminus 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

线粒体促凋亡蛋白抗体规格 产品应用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 This gene encodes an inhibitor of apoptosis protein (IAP)-binding protein. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and it moderates the caspase inhibition of IAPs. Multiple polyadenylation sites have been found for this gene. Several alternatively spliced transcript variants that encode distinct isoforms have been described for this gene but the validity of some transcripts, and their predicted ORFs, has not been determined conclusively. The inhibitor of apoptosis (IAP) proteins regulate programmed cell death by inhibiting members of the caspase family of enzymes. A novel mammalian protein that binds to IAPs and neutralizes their inhibitory effect on caspases has been designated Smac/DIABLO. This is a mitochondrial protein that is released along with cytochrome c during apoptosis and activates the cytochrome c/Apaf-1/caspase-9 pathway. Analysis of the structural basis of Smac/DIABLO reveals that the N-terminal amino acids are required for binding of Smac/DIABLO to IAPs and activation of caspases. Smac/DIABLO is expressed in a variety of human and mouse tissues.

Function : Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). Inhibits the activity of BIRC6/bruce by inhibiting its binding to caspases. Isoform 3 attenuates the stability and apoptosis-inhibiting activity of XIAP/BIRC4 by promoting XIAP/BIRC4 ubiquitination and degradation through the ubiquitin-proteasome pathway. Isoform 3 also disrupts XIAP/BIRC4 interacting with processed caspase-9 and promotes caspase-3 activation. Isoform 1 is defective in the capacity to down-regulate the XIAP/BIRC4 abundance.

Subunit : Homodimer. Interacts with NGFRAP1/BEX3 (By similarity). Interacts with BIRC2/c-IAP1, BIRC3/c-IAP2, XIAP/BIRC4, BIRC6/bruce and BIRC7/livin. Interacts with the monomeric and dimeric form of BIRC5/survivin.

Subcellular Location : Mitochondrion. Note=Released into the cytosol when cells undergo apoptosis.

Tissue Specificity : Ubiquitously expressed with highest expression in testis. Expression is also high in heart, liver, kidney, spleen, prostate and ovary. Low in brain, lung, thymus and peripheral blood leukocytes. Isoform 3 is ubiquitously expressed.

Post-translational modifications : Ubiquitinated by BIRC7/livin.

DISEASE : Deafness, autosomal dominant, 64 (DFNA64) [MIM:614152]: A form of non-syndromic sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. Note=The disease is caused by mutations affecting the gene represented in this entry.

Database links :

UniProtKB/Swiss-Prot: Q9NR28.1

Entrez Gene: 56616 Human

Entrez Gene: 66593 Mouse

Entrez Gene: 288753 Rat

Omim: 605219 Human

SwissProt: Q9NR28 Human

SwissProt: Q9JIQ3 Mouse

Unigene: 169611 Human

Unigene: 46716 Mouse

Smac（second mitochondria-derived activator of caspase）又称DIABLO（direct IAP binding protein），是一种线粒体蛋白，也是重要的促细胞凋亡蛋白之一，能够凋亡，当杀死不正常细胞的机制启动后，线粒体释放出Smac蛋白促使受损或不正常细胞凋亡的生化过程就会出现，Smac在诱导细胞凋亡，增加细胞的敏感性及*等方面有一定的意义和应用前景。Smac是第二个线粒体来源的胱氨酸酶激活剂，它通过拮抗或中和一种或几种凋亡抑制蛋白（IAP）家族成员而发挥其促凋亡作用。

Anti-Survivin/FITC 绿色荧光素标记Survivin蛋白抗体IgGMulti-class antibodies规格： 0.2ml

GSK-3 Beta(NT) (Glycogen Synthase Kinase-3 Beta) 糖原合酶激酶-3β(抗原)Multi-class antibodies规格： 0.5mg

磷酸酯酶-2Ac抗体 Anti-PP2Ac 0.1ml

SPHK1 英文名称： 鞘醇激酶1抗体 0.2ml

EGFL8 英文名称： 表皮生长因子样蛋白8抗体 0.2ml

Rhesus antibody Rh Phospho-MSK1 (Ser376) 磷酸化有丝分裂原和应激活化型蛋白激酶1抗体 规格 0.1ml

GSK-3 Beta(NT) (Glycogen Synthase Kinase-3 Beta) 糖原合酶激酶-3β(抗原)Multi-class antibodies规格： 0.5mg

Anti-PDGF-B/FITC 荧光素标记血小板源性生长因子-B抗体IgGMulti-class antibodies规格： 0.2ml

Rabbit Anti- guinea pig IgG/FITC 荧光素标记兔抗豚鼠IgGMulti-class antibodies规格： 0.3ml

TNF-α膜受体抗体/DR3 死亡受体3抗体 Anti-DR3/TNF-αR/TRAMP 0.1ml

Rabbit Anti-horse IgG/PE-Cy3 PE-Cy3标记的兔抗马IgG 0.1ml

FBXO11 英文名称： F-box蛋白家族FBXO11抗体 0.2ml

Rhesus antibody Rh Phospho-Torc2/Crtc2(Ser171) 磷酸化CREB转录共激活因子TORC2抗体 规格 0.1ml

Rabbit Anti- guinea pig IgG/FITC 荧光素标记兔抗豚鼠IgGMulti-class antibodies规格： 0.3ml

P19ARF p19ARF基因抗原Multi-class antibodies规格： 0.5mg

Anti-ARAlpha1/ADRA1B alpha 1能受体抗体Multi-class antibodies规格： 0.1ml

Rhesus antibody Rh phospho-arfaptin 2(Ser260) 磷酸化ADP核糖基化因子结合蛋白2抗体 规格 0.1ml

TIMP-1 浓缩液 0.1ml 进口分装

Uridine Phosphorylase 1 英文名称： 尿嘧啶核苷磷酸化酶1抗体 0.2ml

DGCR2 英文名称： DGCR2蛋白抗体 0.2ml

Anti-ARAlpha1/ADRA1B alpha 1能受体抗体Multi-class antibodies规格： 0.1ml

IL6 Protein Rat 重组大鼠 IL6 / Ierleukin-6 蛋白

小鼠小肠内皮细胞完全培养基 100mL

CD14 Others Human 人 CD14 人细胞裂解液 (阳性对照)

Pig MSC猪骨髓间质干细胞 Pig MSC of porcine bone marrow mesenchymal stem cells DMEM低糖+10% FBS+glutamin

CL-0135L1210(小鼠细胞)5×106cells/瓶×2

RM1(大鼠肌肉成纤维样细胞) 5×106cells/瓶×2 PC-12未分化(大鼠肾上腺嗜铬细胞瘤)

CM-M095小鼠成年表皮角质形成层细胞完全培养基100mL

KLK1 Others Mouse 小鼠 KLK1 / Kallikrein 1 人细胞裂解液 (阳性对照)

星形细胞培养基SteCM-prf

SW620[SW-620]细胞，人腺癌细胞 人肾上腺腺瘤细胞,NCI-H205细胞 原代系膜细胞培养特制添加剂Many types of cells包装：5/2.5/1ml

猪源细胞

SELPLG Others Human 人 RPSGL-1 人细胞裂解液 (阳性对照)

线粒体促凋亡蛋白抗体规格 IL6 Protein Rat 重组大鼠 IL6 / Ierleukin-6 蛋白

小鼠小肠内皮细胞完全培养基 100mL

CD14 Others Human 人 CD14 人细胞裂解液 (阳性对照)

Pig MSC猪骨髓间质干细胞 Pig MSC of porcine bone marrow mesenchymal stem cells DMEM低糖+10% FBS+glutamin

CL-0135L1210(小鼠细胞)5×106cells/瓶×2

RM1(大鼠肌肉成纤维样细胞) 5×106cells/瓶×2 PC-12未分化(大鼠肾上腺嗜铬细胞瘤)

抗体的生物素化标记实验要点：

1. 线粒体促凋亡蛋白抗体规格 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及苯酚基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

抗体的鉴定：

1）线粒体促凋亡蛋白抗体规格 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。