抗体的生物素化标记实验要点：

1. 三磷酸腺苷结合转运蛋白G超家族成员8抗体说明书 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及苯酚基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

产品订购信息：
英文名称  Anti-STSL/ABCG8 

中文名称   三磷酸腺苷结合转运蛋白G超家族成员8抗体说明书 

别    名  ATP binding cassette sub family G (WHITE) member 8 (sterolin 2); ATP binding cassette sub family G member 8; MGC142217; Sterolin 2; STSL; ABCG8_HUMAN.

浓    度   1mg/1ml

规 格  0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit 

产品类型   一抗  

研究领域    

蛋白分子量  predicted molecular weight: 76kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human ABCG8 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

三磷酸腺苷结合转运蛋白G超家族成员8抗体说明书 产品应用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:100-500 ICC=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. The protein encoded by this gene functions to exclude non-cholesterol sterol entry at the intestinal level, promote excretion of cholesterol and sterols into bile, and to facilitate transport of sterols back into the intestinal lumen. It is expressed in a tissue-specific manner in the liver, intestine, and gallbladder. This gene is tandemly arrayed on chromosome 2, in a head-to-head orientation with family member ABCG5. Mutations in this gene may contribute to sterol accumulation and atherosclerosis, and have been observed in patients with sitosterolemia. [provided by RefSeq, Jul 2008].

Function : Transporter that appears to play an indispensable role in the selective transport of the dietary cholesterol in and out of the enterocytes and in the selective sterol excretion by the liver into bile.

Subunit : May form heterodimers with ABCG5 or be tightly coupled to ABCG5 along a pathway regulating diatery-sterol absorption and excretion.

Subcellular Location : Membrane; Multi-pass membrane protein (Probable).

Tissue Specificity : Strongly expressed in the liver, lower levels in the small intestine and colon. Detectable in a wide variety of human tissues.

DISEASE : Genetic variations in ABCG8 can be associated with susceptibility to gallbladder disease type 4 (GBD4) [MIM:611465]. With an overall prevalence of 10-20%, gallstone disease (cholelithiasis) represents one of the most frequent and economically relevant health problems of industrialized countries.

Defects in ABCG8 are a cause of sitosterolemia (STSL) [MIM:210250]; also known as phytosterolemia or shellfish sterolemia. It is a rare autosomal recessive disorder characterized by increased intestinal absorption of all sterols including cholesterol, plant and shellfish sterols, and decreased biliary excretion of dietary sterols into bile. Sitosterolemia patients have hypercholesterolemia, very high levels of plant sterols in the plasma, and frequently develop tendon and tuberous xanthomas, accelerated atherosclerosis and premature coronary artery disease.

Similarity : Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.

Contains 1 ABC transmembrane type-2 domain.

Contains 1 ABC transporter domain.

Database links : UniProtKB/Swiss-Prot: Q9H221.1

抗体的鉴定：

1）三磷酸腺苷结合转运蛋白G超家族成员8抗体说明书 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。

Anti-UMOD/FITC 荧光素标记UMOD抗体IgGMulti-class antibodies规格： 0.2ml

CXCL10/IP-10 CXCL10趋化因子10/干扰素诱导蛋白10抗原Multi-class antibodies规格： 0.5mg

组织蛋白酶H抗体 Anti-cath-H/CH 0.1ml

Sec8 英文名称： 胞吐分泌蛋白Sec8抗体 0.2ml

EDRF1 英文名称： 红细胞分化相关因子1抗体 0.2ml

Rhesus antibody Rh Phospho-Histone H3 (Thr11) 磷酸化组蛋白H3抗体 规格 0.1ml

CXCL10/IP-10 CXCL10趋化因子10/干扰素诱导蛋白10抗原Multi-class antibodies规格： 0.5mg

Anti-Phospho-FAK (Tyr925) /FITC 荧光素标记兔抗人、大、小鼠磷酸化粘着斑激酶抗体IgGMulti-class antibodies规格： 0.2ml

Rabbit Anti-chicken IgG/Gold 金标记兔抗鸡IgG(10nm/15nm)Multi-class antibodies规格： 0.5ml

角质形成细胞生长因子受体/纤维母细胞生长因子-7抗体 Anti- KGF/FGF-7 0.1ml

Rabbit Anti-Goat IgG/PE PE标记的兔抗羊IgG 0.1ml

FOXRED1 英文名称： 单跨膜蛋白FOXRED1抗体 0.2ml

Rhesus antibody Rh Phospho-Syk (Ty*3) 磷酸化非受体型酪氨酸蛋白激酶抗体 规格 0.1ml

Rabbit Anti-chicken IgG/Gold 金标记兔抗鸡IgG(10nm/15nm)Multi-class antibodies规格： 0.5ml

Resistin 抵抗素抗原Multi-class antibodies规格： 0.5mg

Anti-phospho-Bax(Ser184) 磷酸化Bax抗体Multi-class antibodies规格： 0.1ml

Rhesus antibody Rh PHLP 抑制基因PHLPP抗体 规格 0.2ml

Keratin10 浓缩液 0.1ml 进口分装

UVRAG 英文名称： 抑制基因UVRAG抗体 0.2ml

Chorein/VPS13A 英文名称： 液泡蛋白分选蛋白VPS13A抗体 0.1ml

Anti-phospho-Bax(Ser184) 磷酸化Bax抗体Multi-class antibodies规格： 0.1ml

IL1B Protein Cynomolgus 重组食蟹猴 IL-1 beta / IL1B 蛋白

小鼠肺大动脉平滑肌细胞完全培养基 100mL

MFI2 Others Mouse 小鼠 MFI2 / CD228 / melanoansferrin 人细胞裂解液 (阳性对照)

C3H/10T1/2, Clone 8小鼠胚胎成纤维细胞 C3H/10T1/2, Clone 8 mouse embryonic fibroblast MEM培养基(GIBCO)+10%FBS

CL-0345HB611(人细胞)5×106cells/瓶×2

NCI-H520(人肺鳞癌细胞) 5×106cells/瓶×2 T/G HA-VSMC(人主动脉平滑肌细胞)

CM-R037大鼠小肠隐窝上皮细胞完全培养基100mL

IGFBP5 Others Cynomolgus 食蟹猴 IGFBP5 / IGFBP-5 人细胞裂解液 (阳性对照)

人脂肪间充质干细胞HMSC-ad

SK-MES-1细胞，人细胞 人膀胱鳞癌细胞,ScaBER细胞 人前脂肪细胞-皮下HPA-s

水貂肺上皮细胞;Mv.1.Lu(NBL-7)

MICB Others Human 人 MICB 人细胞裂解液 (阳性对照)

三磷酸腺苷结合转运蛋白G超家族成员8抗体说明书 IL1B Protein Cynomolgus 重组食蟹猴 IL-1 beta / IL1B 蛋白

小鼠肺大动脉平滑肌细胞完全培养基 100mL

MFI2 Others Mouse 小鼠 MFI2 / CD228 / melanoansferrin 人细胞裂解液 (阳性对照)

C3H/10T1/2, Clone 8小鼠胚胎成纤维细胞 C3H/10T1/2, Clone 8 mouse embryonic fibroblast MEM培养基(GIBCO)+10%FBS

CL-0345HB611(人细胞)5×106cells/瓶×2

NCI-H520(人肺鳞癌细胞) 5×106cells/瓶×2 T/G HA-VSMC(人主动脉平滑肌细胞)