产品订购信息：
英文名称  Anti-SPFH2 

中文名称   内质网脂质转运相关蛋白2抗体品牌 

别    名  C8orf2; Chromosome 8 open reading frame 2; Endoplasmic reticulum lipid raft associated protein 2; Endoplasmic reticulum lipid raft-associated protein 2; ER lipid raft associated 2; ERLIN 2; Erlin-2; ERLIN2; ERLN2_HUMAN; HGNC:1356; MGC87072; NET32; SPFH 2; SPFH domain containing protein 2; SPFH domain family member 2; SPFH domain-containing protein 2; SPFH2; SPG18; Stomatin prohibitin flotillin HflC/K domain containing protein 2; Stomatin-prohibitin-flotillin-HflC/K domain-containing protein 2.

浓    度   1mg/1ml

规 格  0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep 

产品类型   一抗  

研究领域    细胞生物 神经生物学 信号转导 通道蛋白 表观遗传学

蛋白分子量  predicted molecular weight: 38kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human SPFH2 (201-280aa) 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

内质网脂质转运相关蛋白2抗体品牌 产品应用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 SPFH2 is a ubiquitously expressed 339 amino acid protein that belongs to the band 7/mec-2 family. Localized to lipid raft-like domains in the membrane of the endoplasmic reticulum (ER), SPFH2 plays a crucial role in the ER-associated degradation (ERAD) pathway that removes metabolically regulated and aberrant proteins from the ER. Specifically, SPFH2 associates with IP3R-I (Inositol 1,4,5-triphosphate receptor I), a substrate of the ERAD pathway, and facilitates its polyubiquitination and subsequent degradation. Three isoforms of SPFH2 are expressed due to alternative splicing events.

Function : Component of the ERLIN1/ERLIN2 complex which mediates the endoplasmic reticulum-associated degradation (ERAD) of inositol 1,4,5-trisphosphate receptors (IP3Rs). Also involved in ITPR1 degradation by the ERAD pathway.

Subunit : Interacts with activated ITPR1, independently of the degree of ITPR1 polyubiquitination (By similarity). Forms a heteromeric complex with ERLIN1. In complex with ERLIN1, interacts with RNF170.

Subcellular Location : Endoplasmic reticulum membrane. Associated with lipid raft-like domains of the endoplasmic reticulum membrane.

Tissue Specificity : Ubiquitous.

DISEASE : Defects in ERLIN2 are the cause of spastic paraplegia autosomal recessive type 18 (SPG18) [MIM:611225]. A form of spastic paraplegia, a neurodegenerative disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Rate of progression and the severity of symptoms are quite variable. Initial symptoms may include difficulty with balance, weakness and stiffness in the legs, muscle spasms, and dragging the toes when walking. In some forms of the disorder, bladder symptoms (such as incontinence) may appear, or the weakness and stiffness may spread to other parts of the body. SPG18 is a severe form with onset in early childhood. Most affected individuals have severe psychomotor retardation. Some may develop significant joint contractures.

Similarity : Belongs to the band 7/mec-2 family.

Database links : UniProtKB/Swiss-Prot: O94905.1

Anti-SP-A/FITC 荧光素标记肺表面活性蛋白A抗体IgGMulti-class antibodies规格： 0.2ml

DDC (DOPA decarboxylase) 多巴胺脱羧酶(抗原)Multi-class antibodies规格： 0.5mg

原活化蛋白激酶p38抗体 Anti-P38 MAPK 0.1ml

surface layer protein 英文名称： 乳酸细菌表面蛋白抗体 0.2ml

ECT2 英文名称： 上皮细胞癌转化蛋白2抗体 0.1ml

Rhesus antibody Rh phospho-NFKBIA(Se*/36) 磷酸化IKB alpha(Se*/36)抗体 规格 0.1ml

DDC (DOPA decarboxylase) 多巴胺脱羧酶(抗原)Multi-class antibodies规格： 0.5mg

Anti-Phospho-Progesterone Receptor (Ser190)/FITC 荧光素标记磷酸化孕激素受体抗体IgGMulti-class antibodies规格： 0.2ml

Rabbit Anti-dog IgM/Gold 金标记兔抗狗IgM (10nm/15nm)Multi-class antibodies规格： 2ml

角质形成细胞生长因子受体抗体 Anti-KGFR 0.2ml

Mouse Anti-Guinea pig IgG/PE-Cy7 PE-Cy7标记的小鼠抗豚鼠IgG 0.1ml

FOXH1 英文名称： 叉头蛋白H1抗体 0.2ml

Rhesus antibody Rh Phospho-Stathmin(Ser25) 磷酸化原癌基因蛋白18 规格 0.1ml

Rabbit Anti-dog IgM/Gold 金标记兔抗狗IgM (10nm/15nm)Multi-class antibodies规格： 2ml

JAK1(Tyrosine-protein kinase JAK1; Janus kinase 1) 蛋白质酪氨酸激酶JAK-1抗原Multi-class antibodies规格： 0.5mg

Anti-phospho-ADRB2(Ser346) 磷酸化能受体β2抗体Multi-class antibodies规格： 0.1ml

Rhesus antibody Rh phospho-CBL2(Tyr774) 磷酸化原癌蛋白CBL2抗体 规格 0.1ml

单组分AEC染色试剂盒 200片/20ml 需用甘油封固,红色

VAPB 英文名称： 囊泡相关膜蛋白相关的蛋白B 0.2ml

DNA polymerase mu 英文名称： DNA聚合酶μ/DNA pol μ抗体 0.2ml

Anti-phospho-ADRB2(Ser346) 磷酸化能受体β2抗体Multi-class antibodies规格： 0.1ml

IL1RN Protein Rat 重组大鼠 IL-1RA / IL1RN 蛋白 (Fc 标签)

大鼠视网膜神经节细胞完全培养基 100mL

HA Others H12N1 甲型 H12N1 (A/mallard duck/Alberta/342/1983) 血凝素 (Hemagglutinin / HA) 人细胞裂解液 (阳性对照)

HL-7702人正常肝细胞 HL-7702 human normal liver cell 1640+10% FBS

CL-0435SF126(人细胞)5×106cells/瓶×2

NCI-H596(人肺腺鳞癌细胞) 5×106cells/瓶×2 cv-1(非洲绿猴肾细胞)

CM-R063大鼠肾小球内皮细胞完全培养基100mL

EPHA3 Others Mouse 小鼠 EphA3 (aa 569-984) 杆状病毒-昆虫细胞裂解液 (阳性对照)

大鼠小脑星形胶质细胞RA-c

非洲绿猴肾细胞;GL-37 细胞，CNE细胞 AsPC-1(转移*腺癌细胞)

EB病毒转化的人B淋巴细胞;HH-01

TNFRSF10B Others Mouse 小鼠 AILR2 / TNFRSF10B 人细胞裂解液 (阳性对照)

内质网脂质转运相关蛋白2抗体品牌 IL1RN Protein Rat 重组大鼠 IL-1RA / IL1RN 蛋白 (Fc 标签)

大鼠视网膜神经节细胞完全培养基 100mL

HA Others H12N1 甲型 H12N1 (A/mallard duck/Alberta/342/1983) 血凝素 (Hemagglutinin / HA) 人细胞裂解液 (阳性对照)

HL-7702人正常肝细胞 HL-7702 human normal liver cell 1640+10% FBS

CL-0435SF126(人细胞)5×106cells/瓶×2

NCI-H596(人肺腺鳞癌细胞) 5×106cells/瓶×2 cv-1(非洲绿猴肾细胞)

抗体的生物素化标记实验要点：

1. 内质网脂质转运相关蛋白2抗体品牌 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及苯酚基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

抗体的鉴定：

1）内质网脂质转运相关蛋白2抗体品牌 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。