抗体的生物素化标记实验要点：

1. 基底膜相关软骨素蛋白多糖抗体品牌 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及苯酚基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

产品订购信息：
英文名称  Anti-SMC3/CSPG6 

中文名称   基底膜相关软骨素蛋白多糖抗体品牌 

别    名  BAM; Bamacan; Basement membrane associated chondroitin proteoglycan; Basement membrane-associated chondroitin proteoglycan; BMH; CDLS3; chondroitin sulfate proteoglycan 6 (bamacan); Chondroitin sulfate proteoglycan 6; Chromosome associated polypeptide; Chromosome-associated polypeptide; CSPG 6; CSPG6; hCAP; SMC 3; SMC protein 3; SMC-3; smc3; SMC3_HUMAN; SMC3L1; Structural maintenance of chromosome 3; Structural maintenance of chromosomes 3; Structural maintenance of chromosomes protein 3.

浓    度   1mg/1ml

规 格  0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Chicken, Dog, Cow, Rabbit 

产品类型   一抗  

研究领域     细胞生物 免疫学 染色质和核信号 细胞周期蛋白 结合蛋白 细胞分化 表观遗传学

蛋白分子量  predicted molecular weight: 142kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human SMC3/CSPG6 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

基底膜相关软骨素蛋白多糖抗体品牌 产品应用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 Central component of cohesin, a complex required for chromosome cohesion during the cell cycle. The cohesin complex may form a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. Cohesion is coupled to DNA replication and is involved in DNA repair. The cohesin complex plays also an important role in spindle pole assembly during mitosis and in chromosomes movement.

Function : Central component of cohesin, a complex required for chromosome cohesion during the cell cycle. The cohesin complex may form a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. Cohesion is coupled to DNA replication and is involved in DNA repair. The cohesin complex plays also an important role in spindle pole assembly during mitosis and in chromosomes movement.

Subunit : Interacts with MXI1, MXD3 and MXD4. Interacts with SYCP2. Found in a complex with SMC1A, CDCA5 and RAD21, PDS5A/APRIN and PDS5B/SCC-112 (By similarity). Forms a heterodimer with SMC1A or SMC1B in cohesin complexes. Cohesin complexes are composed of the SMC1 (SMC1A or SMC1B) and SMC3 heterodimer attached via their hinge domain, RAD21 which link them, and one STAG protein (STAG1, STAG2 or STAG3), which interacts with RAD21. Also found in meiosis-specific cohesin complexes. Interacts with NUMA1, and forms a ternary complex with KIF3B and KIFAP3, suggesting a function in tethering the chromosomes to the spindle pole and in chromosome movement. Interacts with PDS5A and WAPAL; regulated by SMC3 acetylation. Interacts with RPGR (By similarity).

Subcellular Location : Nucleus. Chromosome. Chromosome, centromere. Note=Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of the cohesin complex is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. The phosphorylated form at Ser-1083 is preferentially associated with unsynapsed chromosomal regions (By similarity).

Post-translational modifications : Phosphorylated at Ser-1083 in a SPO11-dependent manner (By similarity).

Acetylation at Lys-105 and Lys-106 by ESCO1 is important for genome stability and S phase sister chromatid cohesion. Regulated by DSCC1, it is required for processive DNA synthesis, coupling sister chromatid cohesion establishment during S phase to DNA replication. Deacetylation by HDAC8, regulates release of the cohesin complex from chromatin.

DISEASE : Cornelia de Lange syndrome 3 (CDLS3) [MIM:610759]: A form of Cornelia de Lange syndrome, a clinically heterogeneous developmental disorder associated with malformations affecting multiple systems. Characterized by facial dysmorphisms, abnormal hands and feet, growth delay, cognitive retardation, hirsutism, gastroesophageal dysfunction and cardiac, ophthalmologic and genitourinary anomalies. Cornelia de Lange syndrome type 3 is a mild form with absence of major structural anomalies. The phenotype in some instances approaches that of apparently non-syndromic mental retardation. Note=The disease is caused by mutations affecting the gene represented in this entry.

Similarity : Belongs to the SMC family. SMC3 subfamily.

Database links : UniProtKB/Swiss-Prot: Q9UQE7.2

抗体的鉴定：

1）基底膜相关软骨素蛋白多糖抗体品牌 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。

Anti-TRF2/FITC 荧光素标记端粒结合蛋白2抗体IgGMulti-class antibodies规格： 0.2ml

Alpha-Synuclein 核突触蛋白(抗原)Multi-class antibodies规格： 0.5mg

促甲状腺素受体抗体 Anti-TSHR 0.1ml

C14orf174 英文名称： 14号染色体开放阅读框174 0.2ml

EBP50 英文名称： 细胞骨架连接质膜蛋白抗体 0.1ml

Rhesus antibody Rh phospho-IRAK4(Thr345) 磷酸化白介素-1受体相关激酶4抗体 规格 0.1ml

Alpha-Synuclein 核突触蛋白(抗原)Multi-class antibodies规格： 0.5mg

Anti-Phospho-Rb (Ser780)/FITC 荧光素标记磷酸化视网膜母细胞瘤相关蛋白1抗体IgGMulti-class antibodies规格： 0.2ml

Rabbit Anti-Guinea IgG Whole serum 兔抗豚鼠IgG抗血清Multi-class antibodies规格： 1ml

KB抑制蛋白抗体 Anti-IKB β 0.2ml

Rabbit anti-Bovine IgG whole serum 兔抗IgG抗血清 1ml

phospho-FANCA (Ser1149) 英文名称： 磷酸化范可尼组蛋白A抗体 0.1ml

Rhesus antibody Rh phospho-PTPRA(Tyr798) 磷酸化酪氨酸磷酸酶α抗体 规格 0.1ml

Rabbit Anti-Guinea IgG Whole serum 兔抗豚鼠IgG抗血清Multi-class antibodies规格： 1ml

Integrin Alpha3 整合素α3抗原Multi-class antibodies规格： 0.5mg

Anti-ADH/AVP 抗激素/升压素抗体Multi-class antibodies规格： 0.2ml

Rhesus antibody Rh Phospho-cdc25A (Ser292) 磷酸化细胞分裂周期蛋白25抗体 规格 0.1ml

核快红染色液-浅红色 6ml Sigma原料

VCP 英文名称： 含缬酪肽蛋白抗体 0.1ml

phospho-Dynamin 1 (Ser774) 英文名称： 磷酸化酶动力蛋白1抗体 0.1ml

Anti-ADH/AVP 抗激素/升压素抗体Multi-class antibodies规格： 0.2ml

IFNG Protein Mouse 重组小鼠 IFNG / Ierferon Gamma 蛋白 (His 标签)

大鼠神经胶质细胞完全培养基 100mL

GDNF Others Human 人 GDNF 人细胞裂解液 (阳性对照)

MKN-45人细胞 Human gasic cancer cells MKN-45 1640+10% FBS

MGC80-3(MGC-803)人细胞

NCI-H157(人非小细胞) 5×106cells/瓶×2 SRSV/3T3(SRSV转化的3T3细胞)

CM-R095大鼠成年表皮角质形成层细胞完全培养基100mL

NP Others H7N9 甲型 H7N9 (A/Shanghai/1/2013) Nucleocapsid / NP 杆状病毒-昆虫细胞裂解液 (阳性对照)

小鼠胚胎成纤维细胞-处理MEF-mt

非洲绿猴肾细胞;Vero E6 人类恶性，A375细胞 LS 174T(腺癌细胞)

EB病毒转化的人B淋巴细胞;KMY0922

DMBT1 Others Human 人 DMBT1 / GP340 人细胞裂解液 (阳性对照)

基底膜相关软骨素蛋白多糖抗体品牌 IFNG Protein Mouse 重组小鼠 IFNG / Ierferon Gamma 蛋白 (His 标签)

大鼠神经胶质细胞完全培养基 100mL

GDNF Others Human 人 GDNF 人细胞裂解液 (阳性对照)

MKN-45人细胞 Human gasic cancer cells MKN-45 1640+10% FBS

MGC80-3(MGC-803)人细胞

NCI-H157(人非小细胞) 5×106cells/瓶×2 SRSV/3T3(SRSV转化的3T3细胞)