全、新、优、品、好四大特点：

丝氨酸/苏氨酸蛋白激酶SIK1抗体规格 全：公司提供上万种产品，涵盖了生物试剂，elisa试剂盒，标准品，培养基，原装耗材，抗体、培养基、ATCC细胞等，基本上各种科研所需产品在我司都能找到。

新：产品更新速度较快，基本上每周都有新产品出现。

优：产品质量好，投诉比较少。

好：我公司具有优质的技术团队，产品一旦售出，实验过程中遇到困难可提供在线技术咨询。使您使用产品时没有任何的后顾之忧。

丝氨酸/苏氨酸蛋白激酶SIK1抗体规格 英文名称  Anti-Snf1lk/SIK1

中文名称  丝氨酸/苏氨酸蛋白激酶SIK1抗体规格 

别    名 MSK; myocardial SNF1 like kinase; Salt inducible kinase 1; Salt-inducible protein kinase 1; Serine threonine protein kinase SNF1 like kinase 1; Serine threonine protein kinase SNF1LK; Serine/threonine-protein kinase SIK1; Serine/threonine-protein kinase SNF1-like kinase 1; Serine/threonine-protein kinase SNF1LK; SIK-1; SIK1; SIK1_HUMAN; SNF1 like kinase.

产品属性：

浓    度  1mg/1ml

规 格  0.2ml/200μg

抗体来源  Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Dog, Pig, Horse

产品类型   一抗  

研究领域     细胞生物 免疫学 信号转导 细胞周期蛋白 转录调节因子

蛋白分子量  predicted molecular weight: 85kDa 

性    状   Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from human Snf1lk

亚    型  IgG

纯化方法   affinity purified by Protein A

储 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

丝氨酸/苏氨酸蛋白激酶SIK1抗体规格 产品应用   WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 Transient role during the earliest stages of myocardial cell differentiation and/or primitive chamber formation and may also be important for the earliest stages of skeletal muscle growth and/or differentiation. Potential role in G2/M cell cycle regulation. Inhibits CREB activity by phosphorylating and repressing the CREB-specific coactivators, CRTC1-3.

Function : Serine/threonine-protein kinase involved in variousprocesses such as cell cycle regulation, gluconeogenesis andlipogenesis regulation, muscle growth and differentiation and tumorsuppression. Phosphorylates HDAC4, HDAC5, PPME1, SREBF1,TORC1/CRTC1 and TORC2/CRTC2. Acts as a tumor suppressor and plays akey role in p53/TP53-dependent anoikis, a type of apoptosistriggered by cell detachment: required for phosphorylation ofp53/TP53 in response to loss of adhesion and is able to suppressmetastasis. Part of a sodium-sensing signaling network, probably bymediating phosphorylation of PPME1: following increases inintracellular sodium, SIK1 is activated by CaMK1 and phosphorylatesPPME1 subunit of protein phosphatase 2A (PP2A), leading todephosphorylation of sodium/potassium-transporting ATPase ATP1A1and subsequent increase activity of ATP1A1. Acts as a regulator ofmuscle cells by phosphorylating and inhibiting class II histonedeacetylases HDAC4 and HDAC5, leading to promote expression of MEF2target genes in myocytes. Also required during cardiomyogenesis byregulating the exit of cardiomyoblasts from the cell cycle viadown-regulation of CDKN1C/p57Kip2. Acts as a regulator of hepaticgluconeogenesis by phosphorylating and repressing the CREB-specificcoactivators TORC1/CRTC1 and TORC2/CRTC2, leading to inhibit CREBactivity. Also regulates hepatic lipogenesis by phosphorylating andinhibiting SREBF1.

Subunit : Interacts with ATP1A1 (By similarity). Interacts (when phosphorylated on Thr-182 and Ser-186) with YWHAZ.

Subcellular Location : Cytoplasm. Nucleus.

Post-translational modifications : Phosphorylated at Thr-182 by STK11/LKB1 in complex withSTE20-related adapter-alpha (STRADA) pseudo kinase and CAB39,leading to its activation. Phosphorylation at Thr-182 promotesautophosphorylation at Ser-186, which is required for sustainedactivity. Autophosphorylation at Ser-186 is maintained bysequential phosphorylation at Thr-182 by GSK3-beta. GSK3-betacannot initiate phosphorylation at Thr-182, it can only maintainit. Phosphorylation at Ser-575 by PKA promotes translocation to thecytoplasm. Phosphorylation at Thr-322 by CaMK1 followingintracellular sodium concentration leads to activation.Phosphorylated upon DNA damage, probably by ATM or ATR.

DISEASE : Note=Defects in SIK1 may be associated with some cancers,such as breast cancers. Loss of SIK1 correlates with poor patientoutcome in breast cancers (PubMed:19622832).

Similarity : Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. AMPK subfamily.

Contains 1 protein kinase domain.

Contains 1 UBA domain.

Database links : UniProtKB/Swiss-Prot: P57059.2

实验流程：

正在热销的相关产品：

Anti-TBX2/T-box2/FITC 荧光素标记新型基因抗体IgGMulti-class antibodies规格： 0.2ml

Integrin Beta chain (ITG- Beta3/ITGB3) 整合素-β3(抗原)Multi-class antibodies规格： 0.5mg

臂板蛋白3F抗体 Anti-Sema3F 0.2ml

SKP2 英文名称： 细胞S期激酶相关蛋白2抗体 0.1ml

EBP1 英文名称： erbB3结合蛋白1抗体 0.2ml

Rhesus antibody Rh phospho-MEF2C(Ser387) 磷酸化肌细胞增强因子2C抗体 规格 0.1ml

Integrin Beta chain (ITG- Beta3/ITGB3) 整合素-β3(抗原)Multi-class antibodies规格： 0.5mg

Anti-phospho c-kit/SCFR/CD117(pTyr936)FITC 荧光素标记抗磷酸化原癌基因c-kit抗体IgGMulti-class antibodies规格： 0.2ml

Rabbit Anti-Guinea IgG 兔抗豚鼠IgG (亲和层析纯化)Multi-class antibodies规格： 1mg

胰素样肽-2抗体 Anti-GLP-2 0.2ml

Rabbit Anti-Bovine IgM/PE PE标记的兔抗IgM 0.1ml

Factor VIII 英文名称： 凝血因子8/第八凝血因子/第八因子相关抗原抗体 0.1ml

Rhesus antibody Rh Phospho-SHIP1 (Tyr1020) 磷酸化SH2结构含磷酸肌醇SHIP1抗体 规格 0.1ml

Rabbit Anti-Guinea IgG 兔抗豚鼠IgG (亲和层析纯化)Multi-class antibodies规格： 1mg

Inhibin beta B 抑制素βB抗原Multi-class antibodies规格： 0.5mg

Anti-ADAMTS12 整合素样金属蛋白酶与凝血酶1型-12抗体Multi-class antibodies规格： 0.2ml

Rhesus antibody Rh Phospho-cdc2* (Ser76) 磷酸化细胞分裂周期蛋白25抗体 规格 0.1ml

吉姆萨染色液 10ml Sigma原料

VDAC 英文名称： 等电压依赖性阴离子通道抗体 0.2ml

Dynamin 1 英文名称： 酶动力蛋白1抗体 0.1ml

Anti-ADAMTS12 整合素样金属蛋白酶与凝血酶1型-12抗体Multi-class antibodies规格： 0.2ml

IFNA14 Protein Mouse 重组小鼠 IFNA14 / Ierferon alpha-14 蛋白 (Fc 标签)

大鼠神经元细胞完全培养基 100mL

CD40LG Others Mouse 小鼠 CD40L / CD154 / TNFSF5 人细胞裂解液 (阳性对照)

OK负鼠肾细胞 OK opossum kidney cells MEM培养基(GIBCO)+10%FBS

MiaPaCa-2, 人*癌细胞

MS751(人子宫颈表皮癌细胞) 5×106cells/瓶×2 Ana-1(巨噬细胞)

CM-R097大鼠皮下脂肪细胞完全培养基100mL

CTLA4 Others Cynomolgus 食蟹猴 / 恒河猴 CTLA4 / CD152 人细胞裂解液 (阳性对照)

小鼠颗粒细胞MGC

UI-1细胞，人细胞 大鼠小肠隐窝上皮细胞,IEC-6细胞 人气管平滑肌细胞HTSMC

HOS(人细胞) 5×106cells/瓶×2

22RV1(人细胞) 5×106cells/瓶×2 CM-H043人肝实质细胞完全培养基100mL 人骨骼肌成肌细胞RNAHSkMM miRNA5 μg

丝氨酸/苏氨酸蛋白激酶SIK1抗体规格 IFNA14 Protein Mouse 重组小鼠 IFNA14 / Ierferon alpha-14 蛋白 (Fc 标签)

大鼠神经元细胞完全培养基 100mL

CD40LG Others Mouse 小鼠 CD40L / CD154 / TNFSF5 人细胞裂解液 (阳性对照)

OK负鼠肾细胞 OK opossum kidney cells MEM培养基(GIBCO)+10%FBS

MiaPaCa-2, 人*癌细胞

MS751(人子宫颈表皮癌细胞) 5×106cells/瓶×2 Ana-1(巨噬细胞)

技术外包服务:

★丝氨酸/苏氨酸蛋白激酶SIK1抗体规格 分子生物学：质粒抽提、PCR、Q-PCR、RT-PCR、分子生物学：基因合成、引物合成、基因测序、载体构建等

★蛋白工程：原核、哺乳动物蛋白表达系统等

★病毒包装：腺病毒、慢病毒等

★抗体工程：磁珠分选、病理染色、WB、ELISA、IP、IF、IHC、FACS、Confocal等等

★细胞工程：细胞表型分析(凋亡、增殖、周期、迁移、侵袭、修复、克隆形成)、细胞培养、细胞膜制备、稳定细胞株构建、细胞RNAi技术等等。