产品订购信息：
英文名称  Anti-phospho-SCNN1B(Ser633) 

中文名称   磷酸化上皮钠通道β2抗体品牌 

别    名  SCNN1B(phospho S633); Amiloride sensitive sodium channel subunit beta; Beta NaCH; ENaC beta; ENaCB; Epithelial Na(+) channel subunit beta; Epithelial Na+ channel beta subunit; Epithelial Na+ channel subunit beta; Epithelial sodium channel beta 2 subunit; Epithelial sodium channel beta 3 subunit; Nonvoltage gated sodium channel 1 beta subunit; Nonvoltage gated sodium channel 1 subunit beta; SCNEB; SCNN 1B; SCNNB_HUMAN; Sodium channel nonvoltage gated 1 beta (Liddle syndrome); Sodium channel nonvoltage gated 1 beta; SCNNB_HUMAN.

浓    度   1mg/1ml

规 格  0.1ml/100μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Dog, Pig, Cow, Guinea Pig, G 

产品类型   一抗 磷酸化抗体  

研究领域     免疫学 神经生物学 细胞膜受体

蛋白分子量  predicted molecular weight: 73kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated Synthesised phosphopeptide derived from human SCNN1B around the phosphorylation site of Ser633 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

磷酸化上皮钠通道β2抗体品牌 产品应用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 SCNN1B is a subunit of the epithelial sodium channel, ENaC. ENac has high sodium selectivity, low conductance, and amiloride sensitivity. The functional channel of ENaC is composed of at least 3 subunits, alpha (SCNN1A), beta (SCNN1B), and gamma (SCNN1G). The 3 subunits show sequence similarities to one another, indicating descent from a common ancestral gene. Each encodes a protein containing 2 transmembrane domains, with intracellular amino and carboxyl termini.

Function : Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception.

Subunit : Probable heterotrimer containing one alpha, one beta and one gamma subunit. A delta subunit can replace the alpha subunit. Interacts with the WW domains of NEDD4, NEDD4L, WWP1 and WWP2. Interacts with the full length immature form of PCSK9 (pro-PCSK9).

Subcellular Location : Apical cell membrane; Multi-pass membrane protein. Note=Apical membrane of epithelial cells.

Post-translational modifications : Phosphorylated on serine and threonine residues (By similarity).

DISEASE : Pseudohypoaldosteronism 1, autosomal recessive (PHA1B) [MIM:264350]: A rare salt wasting disease resulting from target organ unresponsiveness to mineralocorticoids. PHA1B is a severe form involving multiple organ systems, and characterized by an often fulminant presentation in the neonatal period with dehydration, hyponatremia, hyperkalemia, metabolic acidosis, failure to thrive and weight loss. Note=The disease is caused by mutations affecting the gene represented in this entry. The degree of channel function impairment differentially affects the renin-aldosterone system and urinary Na/K ratios, resulting in distinct genotype-phenotype relationships in PHA1 patients. Loss-of-function mutations are associated with a severe clinical course and age-dependent hyperactivation of the renin-aldosterone system. This feature is not observed in patients with missense mutations that reduce but do not eliminate channel function. Markedly reduced channel activity results in impaired linear growth and delayed puberty (PubMed:18634878).

Liddle syndrome (LIDDS) [MIM:177200]: Autosomal dominant disorder characterized by pseudoaldosteronism and hypertension associated with hypokalemic alkalosis. The disease is caused by constitutive activation of the renal epithelial sodium channel. Note=The disease is caused by mutations affecting the gene represented in this entry.

Bronchiectasis with or without elevated sweat chloride 1 (BESC1) [MIM:211400]: A debilitating respiratory disease characterized by chronic, abnormal dilatation of the bronchi and other cystic fibrosis-like symptoms in the absence of known causes of bronchiectasis (cystic fibrosis, autoimmune diseases, ciliary dyskinesia, common variable immunodeficiency, foreign body obstruction). Clinical features include sub-normal lung function, sinopulmonary infections, chronic productive cough, excessive sputum production, and elevated sweat chloride in some cases. Note=The disease is caused by mutations affecting the gene represented in this entry.

Similarity : Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family. SCNN1B subfamily.

Database links :

UniProtKB/Swiss-Prot: P51168.2

Entrez Gene: 6338 Human

Omim: 600760 Human

SwissProt: P51168 Human

Unigene: 414614 Human

Anti-TRAF2/TNF-R2 /FITC 荧光素标记坏死因子受体相关因子2抗体IgGMulti-class antibodies规格： 0.2ml

BRCA2(Breast cancer susceptbility gene 2) 癌易感基因2抗原Multi-class antibodies规格： 0.5mg

坏死因子相关凋亡诱导配体/凋亡素2配体抗体 Anti-TRAIL/Apo2L 0.1ml

SPRR1a 英文名称： 角质蛋白α抗体 0.1ml

EBF2 英文名称： 早期B淋巴细胞因子2抗体 0.2ml

Rhesus antibody Rh phospho-IRS1(Tyr1222) 磷酸化受体底物-1抗体 规格 0.1ml

BRCA2(Breast cancer susceptbility gene 2) 癌易感基因2抗原Multi-class antibodies规格： 0.5mg

Anti-Phospho-Rb (Ser807/Ser811)/FITC 荧光素标记磷酸化视网膜母细胞瘤相关蛋白1抗体IgGMulti-class antibodies规格： 0.2ml

Rabbit Anti-Guinea IgM Whole serum 兔抗豚鼠IgM抗血清Multi-class antibodies规格： 1ml

KB抑制蛋白α抗体 Anti-IKB α 0.2ml

Goat anti-Bovine IgG whole serum 羊抗IgG抗血清 1ml

FAM134B 英文名称： FAM134B蛋白抗体 0.2ml

Rhesus antibody Rh phospho-PTPRA(Ser180) 磷酸化酪氨酸磷酸酶α抗体 规格 0.1ml

Rabbit Anti-Guinea IgM Whole serum 兔抗豚鼠IgM抗血清Multi-class antibodies规格： 1ml

IRF-1(Interferon regulatory factor-1) 干扰素调节因子抗原Multi-class antibodies规格： 0.5mg

Anti-ADNP/NAP 活性依赖的神经保护肽抗体Multi-class antibodies规格： 0.2ml

Rhesus antibody Rh Phospho-cdc2 (Ser39) 磷酸化周期素依赖激酶2抗体 规格 0.1ml

品名 规格 备注

VE Cadherin 英文名称： 上皮型钙粘附分子抗体 0.1ml

DREF 英文名称： DNA复制相关元件结合因子抗体 0.2ml

Anti-ADNP/NAP 活性依赖的神经保护肽抗体Multi-class antibodies规格： 0.2ml

IFNB1 Protein Mouse 重组小鼠 IFNB1 / IFN-beta / Ierferon beta 蛋白 (Fc 标签)

大鼠脑内皮细胞完全培养基 100mL

FCGRT & B2M Others Mouse 小鼠 FCGRT & B2M Heterodimer 人细胞裂解液 (阳性对照)

LLC-PK1猪肾近曲小管上皮细胞 LLC-PK1 porcine kidney proximal tubular epithelial cells M199+3% FBS/DMEM+F12+12%FBS

C6, 大鼠脑细胞

MDA-MB-468-06(人癌细胞) 5×106cells/瓶×2 Y1(肾上腺皮质细胞)

CM-R103大鼠脑平滑肌细胞完全培养基100mL

CHST11 Others Human 人 CHST11 / C4ST-1 杆状病毒-昆虫细胞裂解液 (阳性对照)

小鼠小胶质细胞MM

SV40转化的非洲绿猴肾细胞;COS-1 膀胱变移细胞癌，T-24细胞 U251(细胞)

EB病毒转化的人B淋巴细胞;KMY0927

EFNB2 Others Mouse 小鼠 EFNB2 / EphrinB2 人细胞裂解液 (阳性对照)

磷酸化上皮钠通道β2抗体品牌 IFNB1 Protein Mouse 重组小鼠 IFNB1 / IFN-beta / Ierferon beta 蛋白 (Fc 标签)

大鼠脑内皮细胞完全培养基 100mL

FCGRT & B2M Others Mouse 小鼠 FCGRT & B2M Heterodimer 人细胞裂解液 (阳性对照)

LLC-PK1猪肾近曲小管上皮细胞 LLC-PK1 porcine kidney proximal tubular epithelial cells M199+3% FBS/DMEM+F12+12%FBS

C6, 大鼠脑细胞

MDA-MB-468-06(人癌细胞) 5×106cells/瓶×2 Y1(肾上腺皮质细胞)

抗体的生物素化标记实验要点：

1. 磷酸化上皮钠通道β2抗体品牌 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及苯酚基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

抗体的鉴定：

1）磷酸化上皮钠通道β2抗体品牌 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。